Cytation C10 Confocal Imaging Reader

Cell Imaging & Microscopy > Confocal Imaging Reader

Overview:

Cytation™ C10 Confocal Imaging Reader combines automated digital confocal and widefield microscopy with conventional multi-mode microplate reading in a unique, patented design. The spinning disk confocal module provides exquisite resolution and optical sectioning capabilities in a wide variety of sample types. High quality components used in the system, including a Hamamatsu scientific CMOS (sCMOS) camera, Olympus objectives and laser-based illumination enable excellent image quality, at a significantly lower price than others. Cytation C10 also includes widefield fluorescence, brightfield and phase contrast optics. Its variable bandwidth monochromator-based multi-mode plate reading is based on the proven design of the market-leading BioTek Synergy™ products. Environmental controls enable live cell assays, and Gen5™ software is specifically designed to make sample detection and image capture quick and effortless. The new 3D viewer provides users with 3D reconstruction of their thicker samples captured with the confocal microscope. Cytation C10 brings affordable confocal to every laboratory.

Features:

Compact, affordable confocal imager for every laboratory

Expertise gained over several years of Cytation development, along with customer feedback, resulted in the Cytation C10.... an automated confocal microscope with excellent performance at a truly attainable price.

Confocal: Improved image quality and analysis

Confocal microscopy can enable you to see a level of detail in your samples that is not possible with widefield optics. Not only can you obtain improved image quality, you can get improved quantification and analysis with confocal images and Gen5 software.

High quality optical components

High quality objectives, filters and other components including the Hamamatsu sCMOS Orca camera, Semrock filters, Olympus objectives and other well-known brands, are used in Cytation C10, enabling the capture of stunning, publication-quality images.

Confocal imaging and multi-mode plate reader in one

With a combination of spinning disk confocal and widefield imaging, plus multi-mode reader, Cytation C10 is truly ready for any assay. And since Cytation C10 is a modular, upgradable instrument, you can get the functionality you need today and add modules later as your needs expand.

Confocal plus widefield = stunning images and analysis

Cytation C10 captures stunning detail in a wide variety of sample types. Use widefield imaging for faster acquisition of large samples at lower magnification, switch to confocal to image small intracellular details or 3D samples. Or combine both modes for highly multiplexed, multi-parameter imaging experiments.

Environmental controls for live cell imaging

Successful live cell kinetic imaging relies on a consistent environment, including temperature control and CO₂/O₂control and monitoring. Cytation C10 provides the perfect environment to grow and analyze live cells over time. Powerful movie maker and kinetic analysis software tools allow visualizing and analysis time-lapse experiments.

Hit-picking: Multi-mode detection + imaging saves time and data storage

(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.

Imaging data sets can take a long time to acquire and require large data storage capacity. The unique hit-picking function takes advantage of the embedded plate reader optics. Set the hit picking criteria, quickly pre-screen the microplate with the plate reader optics and Cytation C10 will automatically image the samples that meet your criteria, saving both time and hard drive space.

Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation use a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance.

Cytation C10: Ready for any application

The combination of confocal and widefield imaging with multi-mode detection will transform your laboratory workflows and increase productivity. Cytation C10 is truly ready for any application.

For Research Use Only. Not for use in diagnostic procedures.

Models:

Part #	C10PHC2	C10MPHC2	C10PWC	C10MPWC	C10PW	C10MPW
Confocal 60 µm spinning disk	•	•	•	•
Confocal 40 µm spinning disk	•	•
Hamamatsu sCMOS camera	•	•
Sony CMOS camera			•	•	•	•
Multi-mode reading		•		•		•
Phase contrast	•	•	•	•	•	•

Specifications:

General
Detection modes	UV-Vis absorbance Fluorescence intensity Luminescence
Read methods	Endpoint, kinetic, spectral scanning, well area scanning
Microplate types	Monochromator: 6- to 384-well plates Imaging: 6- to 1536-well plates
Other labware supported	Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer), Take3 Micro-volume plates
Temperature control	4-Zone incubation to 45 °C with Condensation Control
Shaking	Linear, orbital, double-orbital
Software	Gen5 Microplate Reader and Imager Software included Gen5 Secure for 21 CFR Part 11 compliance (option) Gen5 Image+ and Image Prime software available for full image analysis (option)
Automation	BioStack and 3rd party automation compatible
CO₂ and O₂ control (option)	Range: 0 - 20% (CO₂); 1 - 19% (O₂), with optional Gas Controller Models for both CO₂ and O₂ or CO₂ only are available
Imaging - Confocal Microscope
Imaging modes	Fluorescence
Image processing	Z-projection, digital phase contrast, stitching
Camera	Hamamatsu Orca sCMOS, 16-bit grayscale camera or Sony CMOS 16-bit grayscale camera
Objective capacity	6-position automated turret for user-replaceable objectives
Objectives available	20x, 40x, 60x
Image filter cube capacity	4 user-replaceable fluorescence cubes
Imaging filter cubes available	CFP, CY5, DAPI, GFP, RFP, TRITC, brightfield
Laser	6-line
Automated functions	Autofocus, user-trained autofocus, autoexposure, auto-LED intensity
Autofocus method	Image-based autofocus User-trained autofocus Laser autofocus (option)
Positional controls	Software Control Joystick controller (option)
Imaging methods	Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Field of view	Hamamatsu sCMOS: 0.65 mm + 5% at 20x magnification Sony CMOS: 0.70 mm + 5% at 20x magnification
Image collection rate	Laser autofocus, 0 ms delay, 96 wells: 8 mins, 9 secs Software autofocus, 0 ms delay, 96 wells: 12 mins, 1 sec
Imaging - Widefield Microscope
Imaging mode	Fluorescence, phase contrast, color brightfield, user-selectable brightfield/high contrast brightfield
Imaging method	Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Image processing	Z-projection, digital phase contrast, stitching
Camera	Hamamatsu Orca sCMOS, 16-bit grayscale camera or Sony CMOS 16-bit grayscale camera
Objective capacity	6-position automated turret for user-replaceable objectives
Objectives available	1.25x, 2.5x (2.25x eff), 4x,10x, 20x, 40x, 60x
Phase objectives available	4x, 10x, 20x, 40x
Image filter cube capacity	4 user-replaceable fluorescence cubes, plus brightfield channel
Imaging filter cubes available	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Imaging LED cubes available	365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions	Autofocus, autoexposure, auto-LED intensity
Autofocus method	Image-based autofocus User-trained autofocus Laser autofocus (option)
Positional controls	Software Control Joystick controller (option)
Image collection rate	Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes Laser autofocus: 96 wells, 1 color (DAPI), 4x, <3 minutes
Image Analysis Software option	Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure: 21 CFR Part 11 compliant features
Fluorescence Intensity
Light source	Xenon flash
Detector	PMT
Wavelength selection	Quad monochromators (top/bottom)
Wavelength range	250 – 700 nm (900 nm option)
Monochromator bandwidth	Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range	7 decades
Reading speed (kinetic)	96 wells, sweep mode: 10 seconds 384 wells, sweep mode: 20 second
Luminescence
Wavelength range	300 - 700 nm
Dynamic range	>6 decades
Absorbance
Light source	Xenon flash
Detector	Photodiode
Wavelength selection	Monochromator
Wavelength range	230 - 999 nm, 1 nm increments
Monochromator bandwidth	4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range	0 - 4.0 OD
Resolution	0.0001 OD
Pathlength correction	Yes
Monochromator wavelength accuracy	+ 2 nm
Monochromator wavelength repeatability	+ 0.2 nm
OD accuracy	<1% at 3.0 OD
OD linearity	<1% from 0 to 3.0 OD
OD repeatability	<0.5% at 2.0 OD
Stray light	0.03% at 230 nm
Reading speed (kinetic)	96 wells: 10 seconds 384 wells: 20 seconds
Reagent Injectors (option)
Supported detection modes	All modes
Number	2 syringe pumps
Supported labware	6- to 384-well plates, Petri and cell culture dishes
Dead volume	1.1 mL, with back flush
Dispense volume	5 – 1000 µL in 1 µL increments
Plate geometry	6- to 384-well microplates
Dispense accuracy	+1 µL or 2%
Dispense precision	< 2% at 50 - 200 µL
Physical Characteristics
Power	Instrument: External 250W (minimum), 24VDC power supply, compatible with 100-240 VAC @50-60Hz. Optional 6-channel laser light source: External 250W power supply, compatible with 100- 240VAC @ 50-60Hz. Optional Hamamatsu scientific camera: External 75W power supply, compatible with 100- 240VAC @ 50-60Hz.
Dimensions	27" W x 18.5" H x 20" D (68.6 cm x 45.72 cm x 50.8 cm)
Weight	122 lbs (53.3 Kg)
Regulatory
Regulatory	CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.