Cytation 7 Cell Imaging Multi-Mode Reader

Cell Imaging & Microscopy > Cell Imaging Multi-Mode Readers

Overview:

Cytation™ 7 Cell Imaging Multi-Mode Reader combines automated digital upright and inverted widefield microscopy with conventional multi-mode microplate reading in a unique, patented design. The inverted microscopy module provides sample visualization from 1.25x to 60x magnification in fluorescence, brightfield and color brightfield for a broad range of applications. The upright microscopy module with reflected light imaging enables even more applications such as ELISpot or fast slide scanning and ROI detection workflow. Cytation 7 includes continually variable bandpass monochromators for versatility and performance for general multi-mode plate reader applications. Temperature control to 45 °C, optional Peltier Cooling Module, gas control and shaking expand the applications for kinetic cell based assays. Cytation 7 is controlled by Gen5™ software, which combines ease-of-use with powerful processing and analysis capabilities.

Features:

Multi-mode plate reader with sophisticated imaging

Cytation 7 includes both upright and inverted microscopy optics which opens up a wide range of cellular and reflected light applications that cannot be performed on a standard plate reader. Information on cell morphology, localization of signal, cell count, object identification and quantification can be obtained with Cytation 7’s imaging modes. The monochromator plate reader optics allows running all of the standard plate reader assays.

Cytation 7: Ready for any assay

With its combination of hybrid plate reader and advanced microscopy mode, Cytation is truly ready for any assay. Contact us to learn how Cytation can transform your lab and greatly increase your productivity.

Comprehensive imaging solution

Cytation 7’s inverted microscopy module supports fluorescence, brightfield and color brightfield from 1.25x to 60x to analyze both large objects and intracellular details.
Cytation 7’s upright reflected light imaging module enables a broad range of applications such as ELISpot, colony counting, material inspection, and much more.

Hit-picking: Multi-mode detection + imaging saves time and data storage

(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.

ELISpot imaging

Cytation 7’s upright imaging module can be used to automate assays such as ELISpot in which cell secretions are rendered visible through the use of a colorimetric reaction. Cytation 7 fully automates imaging acquisition, processing, image analysis and object count.

Powerful functionality with ROI identification feature

Cytation 7 and Gen5 software facilitate ROI identification. Cytation 7 scans samples at low magnification before prompting the user to identify regions of interest to be imaged at high magnification. This greatly accelerates the process of imaging ROIs in batches of complex microscopic samples.

Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation uses a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance.

Micro-volume analysis with Take3 plate

Turn your Cytation 7 into a micro-volume analysis system with Take3 or Take3 Trio. You can run 16 or 48 samples in one run to save a lot of time compared to single-sample devices. Gen5 is pre-programmed for ssDNA, dsDNA, RNA and protein quantification in 2 μL.

Models:

Part #	CYT7U	CYT7UW	CYT7UMW	CYT7UM
MICROSCOPY
Upright digital microscope	•	•	•	•
Inverted WFOV microscope		•	•
Upgradable to add inverted microscope	•			•
MULTI-MODE MICROPLATE READING
UV-Vis absorbance			•	•
Fluorescence intensity			•	•
Luminescence			•	•
Upgradable to add multi-mode detection	•	•
OTHER
Take3 Plate compatible			•	•
Incubation to 45 °C	•	•	•	•

Specifications:

General
Detection modes	UV-Vis absorbance Fluorescence intensity Luminescence
Read methods	Endpoint, kinetic, spectral scanning, well area scanning
Microplate types	Monochromator: 6- to 384-well plates Imaging: 6- to 1536-well plates
Other labware supported	Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Take3 Micro-Volume Plates
Temperature control	4-Zone incubation to 45 °C with Condensation Control; +0.5 °C at 37 °C
Cooling	Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.
Shaking	Linear, orbital, double orbital
Software	Gen5™ Microplate Reader and Imager Software included Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation	BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible
CO₂ and O₂ control (option)	Range: 0 - 20% (CO₂); 1 - 19% (O₂) Models for both CO₂ and O₂ or CO₂ only are available
Imaging - Inverted Microscope
Imaging mode	Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield
Imaging method	Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Image processing	Z-projection, digital phase contrast, stitching
Camera	16-bit Sony CMOS wide field of view monochrome camera
Objective capacity	6-position automated turret for user-replaceable objectives
Objectives available	1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Image filter cube capacity	4 user-replaceable fluorescence cubes, plus brightfield channel
Imaging filter cubes available	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Imaging LED cubes available	365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions	Autofocus, user-trained autofocus, autoexposure, auto-LED intensity
Autofocus method	Image-based autofocus User-trained autofocus Laser autofocus (option)
Positional controls	Software Control Joystick controller (option)
Image collection rate	Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes Laser autofocus: 96 wells, 1 color (DAPI), 4x, <3 minutes
Image Analysis Software option	Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure: 21 CFR Part 11 compliant features
Imaging - Upright Microscope
Imaging modes	Reflected color brightfield; transmitted color brightfield
Imaging methods	Single image, montage, time lapse, Z-stacking
Image processing	Z-projection, digital phase contrast, stitching
Camera	16-bit Sony CMOS wide field of view color camera
Lenses	Finder scope, 2x, 4x, 8x
Positional controls	Software Control Joystick controller (option)
Image collection rate	Entire 100 mm dish (montage) @ 1x: <3:00 minutes Entire microscope slide (montage) @ 1x: <1:15 minutes 96-well ELISpot plate @ 1x: <5 minutes
Image Analysis Software option	Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure: 21 CFR Part 11 compliant features
Fluorescence Intensity
Light source	Xenon flash
Detector	PMT (red-shifted PMT option)
Wavelength selection	Quad monochromators (top/bottom)
Wavelength range	250 – 700 (900 nm option)
Monochromator bandwidth	Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range	7 decades
Sensitivity	Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom
Reading speed (kinetic)	96 wells: 11 seconds 384 wells: 22 seconds
Luminescence
Wavelength range	300 - 700 nm
Dynamic range	>6 decades
Sensitivity	20 amol ATP (flash)
Absorbance
Light source	Xenon flash
Detector	Photodiode
Wavelength selection	Monochromator
Wavelength range	230 - 999 nm, 1 nm increment
Monochromator bandwidth	4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range	0 - 4.0 OD
Resolution	0.0001 OD
Pathlength correction	Yes
Monochromator wavelength accuracy	+2 nm
Monochromator wavelength repeatability	+0.2 nm
OD accuracy	<1% at 3.0 OD
OD linearity	<1% from 0 to 3.0 OD
OD repeatability	<0.5% at 2.0 OD
Stray light	0.03% at 230 nm
Reading speed (kinetic)	96 wells: 11 seconds 384 wells: 22 seconds
Reagent Injectors (option)
Number	2 syringe pumps
Supported labware	6- to 384-well plates, Petri and cell culture dishes
Dead volume	1.1 mL with back flush
Dispense volume	5 - 1000 µL in 1 µL increments
Plate geometry	6- to 384-well microplates
Dispense accuracy	+1 µL or 2%
Dispense precision	<2% at 50 - 200 µL
Physical Characteristics
Power	150 Watts maximum consumption
Dimensions	20.2" D x 16.4" W x 17.5" H (51.4 cm D x 41.6 cm W x 44.5 cm H)
Weight	80 lbs (36.3 kg)
Regulatory
Regulatory	CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.