Cell Imaging & Microscopy > Cell Imaging Multi-Mode Readers
BioTek Cytation 7 cell imaging multimode reader combines automated digital upright and inverted widefield microscopy with conventional multimode microplate reading. Cytation 7 includes continually variable bandpass monochromators for versatility and performance for general multimode plate reader applications. The inverted microscopy module provides sample visualization and the upright microscopy module with reflected light imaging enables even more applications, including ELISpot and fast slide scanning. Cytation 7 is controlled by Gen5 software, which combines ease-of-use with powerful processing and analysis capabilities.
(1) Plate reader quickly identifies GFP positive wells. |
Cytation 7 and Gen5 software facilitate ROI identification. Cytation 7 scans samples at low magnification before prompting the user to identify regions of interest to be imaged at high magnification. This greatly accelerates the process of imaging ROIs in batches of complex microscopic samples. |
Turn your Cytation 7 into a microvolume analysis system with Take3 or Take3 Trio. You can run 16 or 48 samples in one run to save a lot of time compared to single-sample devices. Gen5 is pre-programmed for ssDNA, dsDNA, RNA and protein quantification in 2 μL. |
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CYT7U
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CYT7UW |
CYT7UMW
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CYT7UM |
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MICROSCOPY
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Upright digital microscope
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Inverted WFOV microscope
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Upgradable to add inverted microscope
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MULTI-MODE MICROPLATE READING
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UV-Vis absorbance
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Fluorescence intensity
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Luminescence
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OTHER
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Take3 Plate compatible
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Incubation to 45 °C
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General | |
Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence |
Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
Microplate types | Monochromator: 6- to 384-well plates Imaging: 6- to 1536-well plates |
Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Take3 Micro-Volume Plates |
Temperature control | 4-Zone incubation to 45 °C with Condensation Control |
Cooling | Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. |
Shaking | Linear, orbital, double orbital |
Software | Gen5™ Microplate Reader and Imager Software included Gen5 Secure for 21 CFR Part 11 compliance (option) |
Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
CO2 and O2 control (option) | Range: 0 - 20% (CO2); 1 - 19% (O2), with optional Gas Controller Models for both CO2 and O2 or CO2 only are available |
Imaging - Widefield Microscope | |
Imaging mode | Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield |
Imaging method | Single color, multi-color, time lapse, montage, z-stacking, z-stack montage |
Image processing | Z-projection, digital phase contrast, stitching |
Camera | Sony CMOS, 16-bit grayscale |
Objective capacity | 6-position automated turret for user-replaceable objectives |
Objectives available | 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x |
Image filter cube capacity | 4 user-replaceable fluorescence cubes, plus brightfield channel |
Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC |
Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm |
Automated functions | Autofocus, autoexposure, auto-LED intensity |
Autofocus method | Image-based autofocus User-trained autofocus Laser autofocus (option) |
Positional controls | Software Control Joystick controller (option) |
Image collection rate | Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes Laser autofocus: 96 wells, 1 color (DAPI), 4x, <3 minutes |
Image Analysis Software option | Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure: 21 CFR Part 11 compliant features |
Imaging - Upright Microscope | |
Imaging modes | Reflected color brightfield; transmitted color brightfield |
Imaging methods | Single image, montage, time lapse, Z-stacking |
Image processing | Z-projection, digital phase contrast, stitching |
Camera | 16-bit Sony CMOS color camera |
Lenses | Finder scope, 2x, 4x, 8x |
Positional controls | Software Control Joystick controller (option) |
Image collection rate | Entire 100 mm dish (montage) @ 1x: <3:00 minutes Entire microscope slide (montage) @ 1x: <1:15 minutes 96-well ELISpot plate @ 1x: <5 minutes |
Image Analysis Software option | Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure: 21 CFR Part 11 compliant features |
Fluorescence Intensity | |
Light source | Xenon flash |
Detector | PMT |
Wavelength selection | Quad monochromators (top/bottom) |
Wavelength range | 250 – 700 (900 nm option) |
Monochromator bandwidth | Variable, from 9 nm to 50 nm in 1 nm increments |
Dynamic range | 7 decades |
Sensitivity | Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom |
Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
Luminescence | |
Wavelength range | 300 - 700 nm |
Dynamic range | >6 decades |
Sensitivity | 20 amol ATP (flash) |
Absorbance | |
Light source | Xenon flash |
Detector | Photodiode |
Wavelength selection | Monochromator |
Wavelength range | 230 - 999 nm, 1 nm increment |
Monochromator bandwidth | 4 nm (230 - 285 nm), 8 nm (>285 nm) |
Dynamic range | 0 - 4.0 OD |
Resolution | 0.0001 OD |
Pathlength correction | Yes |
Monochromator wavelength accuracy | +2 nm |
Monochromator wavelength repeatability | +0.2 nm |
OD accuracy | <1% at 3.0 OD |
OD linearity | <1% from 0 to 3.0 OD |
OD repeatability | <0.5% at 2.0 OD |
Stray light | 0.03% at 230 nm |
Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
Reagent Injectors (option) | |
Number | 2 syringe pumps |
Supported labware | 6- to 384-well plates, Petri and cell culture dishes |
Dead volume | 1.1 mL with back flush |
Dispense volume | 5 - 1000 µL in 1 µL increments |
Plate geometry | 6- to 384-well microplates |
Dispense accuracy | +1 µL or 2% |
Dispense precision | <2% at 50 - 200 µL |
Physical Characteristics | |
Power | 150 Watts maximum consumption |
Dimensions | 16.4" W x 17.5" H x 20.2" D (41.6 cm x 44.5 cm x 51.4 cm) |
Weight | 80 lbs (36.3 kg) |
Regulatory | |
Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |