Synergy LX Multimode Reader

Microplate Readers: Multi-Mode and Absorbance Readers > Multi-Mode Microplate Readers

Synergy LX Multimode Reader


The BioTek Synergy LX multimode reader automates many common microplate assays. The high-quality optic design ensures excellent data in absorbance, fluorescence, and luminescence detection modes. This affordable system is ideal for many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford, and cell viability. The data display, output to a USB flash drive, printer, or Gen5 software make Synergy LX a versatile assay workstation. The large touch screen user interface and onboard software simplify programming and save time in operation of microplate and microvolume assays.



   Affordable multimode reader

 Automated digital microscopy to 100x

At half the price of similar instruments, Synergy LX is the solution for labs looking for an easy to use microplate reader that offers uncompromised performance for UV-Vis absorbance, fluorescence and luminescence assays.


  Supports common endpoint assays


  Synergy LX, can run the most common assays including:

• A260, A260/A280
• Protein quantification
• Fluorescence, luminescence
• Gene expression
• Cell viability

The independent optical paths ensure uncompromised performance in all modes.
Four imaging modes, multiple imaging processes

   Microvolume quantification with Take3 plates

 Label-free cell counting

Microvolume analysis with BioTek’s Take3 plate and Synergy LX is fast and easy, too. Pre-programmed nucleic acid and protein protocols in Synergy LX display immediate results for up to sixteen, 2 µL samples. Output results to a USB flash drive for use in downstream workflows.


   Continuous UV-Vis wavelength selection: 200 nm to 999 nm


The broad, filter-free wavelength range for absorbance measurements enables many common colorimetric and UV applications, from nucleic acid and protein quantification to turbidity measurements and more.

Automated digital microscopy to 100x

   High performance, high blocking filters for fluorescence and luminescence

Four imaging modes, multiple imaging processes

There’s no compromise on performance in Synergy LX. The high quality filters, coupled with dichroic mirrors in the Synergy LX filter cubes efficiently block background to ensure the best sensitivity for fluorescence measurements.


   Touchscreen: Easy operation, immediate data display


It takes only a few touches to select and run your assay. The data is displayed immediately after the run – a color gradient helps to quickly visualize the data range.

Label-free cell counting

   Output to USB flash drive, printer or Gen5 Software

Four imaging modes, multiple imaging processes

With one touch, export data to a USB flash drive for analysis in BioTek’s Gen5 software or a spreadsheet application.


  Absorbance Fluorescence Luminescence Touchscreen

Synergy LX

Synergy LX touch screen


Synergy LX is available in both non-touch and touchscreen configurations.


Detection modes UV-Vis absorbance
Fluorescence intensity
Read methods Endpoint (onboard software)
Endpoint, kinetic, area scanning, absorbance spectral scanning (under Gen5 control)
Microplate types UV-Vis absorbance: 6- to 384-well plates (onboard software)
Fluorescence intensity and luminescence: 96- and 384-well (onboard software)
All modes: 6- to 384-well microplates (under Gen5 control)
Other labware supported Take3 Micro-Volume Plates (onboard software)
Take3 and Take3 Trio Micro-Volume Plates (under Gen5 control)
Shaking Linear, orbital, double-orbital
Software Endpoint protocols (onboard)
Full data analysis and reporting (under Gen5 control)
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 nm to 999 nm, in 1 nm increments
Monochromator bandwidth < 5 nm
Dynamic range 0 to 4.000 OD
Resolution 0.001 OD (onboard software)
0.0001 OD (under Gen5 control)
Pathlength correction yes
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm (standard deviation)
OD accuracy <1% at 2.0 OD
<3% at 2.5 OD
OD linearity <1% from 0 to 2.5 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 12 seconds
384 wells: 23 seconds
Fluorescence Intensity
Light source Halogen
Detector PMT
Wavelength selection Filters
Wavelength range 320-700 nm (low noise PMT)
320-850 nm (red-shifted PMT)
Dynamic range >6 decades
Sensitivity Fluorescein 2 pM
Reading speed (kinetic) 96 wells: 24 secs
384 wells: 76 secs
Dynamic range >6 decades
Sensitivity 10 amol ATP
Physical Characteristics
Connectivity One USB 2.0 port for computer control
Two USB 2.0 ports for printer connection and USB flash drive (touchscreen configurations only)
Power External 24VDC power supply compatible with 100-240 volts AC @50-60Hz.
60W maximum consumption.
Dimensions 15” H x 15” W x 15” D (with touchscreen)
(38.1 cm H x 38.1 cm W x 38.1 cm D)
12” H x 15” W x 15” D
(30.5 cm H x 38.1 cm W x 38.1 cm D)
Weight < 27 lbs (12.3 kg)
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.