Software
From whole organism imaging to high magnification subcellular image analysis, Gen5 helps transform raw data and images into meaningful results. Gen5 Software automates image capture from BioTek’s Lionheart Automated Microscope and Cytation Cell Imaging Multi-Mode Reader, and automates image processing and analysis to produce publication-ready images and quantitative data. This process of Augmented Microscopy™ enables simple, straightforward imaging workflows.
Augmented Microscopy automates image capture for samples with powerful tools for endpoint and time lapse workflows. Fluorescence, brightfield, color brightfield and phase contrast images are captured in slides, microplates, chamber slides, cell culture dishes for quantitative and qualitative analysis.
Image analysis tools in Gen5 cover a very broad range of application requirements, and are both powerful and easy to use. Analysis functions in Gen5 extend to quantitative data as well. Cell count, confluence, signal translocation, subpopulation analysis and kinetic analysis are just some of the powerful data provided by Gen5’s image analysis tools.
Augmented Microscopy tools include the ability to create publication-ready images, graphs and data using the functions in Gen5 software. There is no need to export images or data to external software to create scatterplots, histograms, EC50 graphs and other data displays for publication.
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Image+
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Image Prime
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Basic Features
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Supports all imaging instrument hardware functionality
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Image Acquisition Modes and Features
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Image up to 4 color channels per image set
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Fast kinetic imaging (up to 10 fps)
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Long-term kinetic imaging (up to 7 days)
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Z-stack imaging of up to 50 slices for thick samples
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Montage imaging for large samples
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Combined z-stack and montage imaging
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Set beacons (imaging regions of interest) per well or vessel
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Image Processing
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| Background flattening to improve image signal to noise | ● | ● | |
| Add user defined image annotations to any image or graph | ● | ● | |
| Record movies of live samples and/or make movies of kinetic image series | ● | ● | |
| Image deconvolution to improve resolution | ● | ● | |
| Digital phase contrast to improve contrast in brightfield images | ● | ● | |
| Cellular Analysis | |||
| Image Statistics (e.g., image total intensity, intensity above/below a threshold) | ● | ● | ● |
| Cell count and confluence on a live sample | ● | ● | ● |
| Cell count and confluence on an acquired image | ● | ● | ● |
| Cellular analysis using a single analysis mask (e.g., object size, shape, area, circularity, intensity) | ● | ● | |
| Analyze subpopulations of cells expressing certain criteria (e.g., size, shape, intensity) | ● | ● | |
| Two analysis masks for advanced measurements (e.g., cytoplasmic signal, signal translocation) | ● | ||
| Advanced cellular analysis optimization tools | ● | ||
| Scatterplots/histograms for visualizing and gating cell level data | ● | ||
| Optional: Spot Counting Module for intracellular objects (e.g., mitochondria, steatosis) | ● |
*For laboratories that require compliance to 21 CFR Part 11, Gen5 Secure editions are available
** For laboratories that require IVD compliance Gen5 IVD editions are available
