Gen5 Software Features for Imaging & Microscopy


Gen5 Software Features for Imaging & Microscopy


From whole organism imaging to high magnification subcellular image analysis, Gen5 helps transform raw data and images into meaningful results. Gen5 Software automates image capture from BioTek’s Lionheart Automated Microscope and Cytation Cell Imaging Multi-Mode Reader, and automates image processing and analysis to produce publication-ready images and quantitative data. This process of Augmented Microscopy™ enables simple, straightforward imaging workflows.

Highly visual workflow interface in Gen5
Highly visual workflow interface in Gen5 3.04



   Automated Image Capture

 Automated Image Capture
 Automated Image Capture

Augmented Microscopy automates image capture for samples with powerful tools for endpoint and time lapse workflows. Fluorescence, brightfield, color brightfield and phase contrast images are captured in slides, microplates, chamber slides, cell culture dishes for quantitative and qualitative analysis.


Exceptional Image Processing Tools

Gen5’s image processing tools provide exceptional processing capability to facilitate the analysis of complex biologies, including 3D samples, large montaged samples and processes including live cell kinetics. From easy brightness/ contrast adjustments to deconvolution and automated movie file creation, image processing in Gen5 enables optimized visualization and analysis for many applications. The independent optical paths ensure uncompromised performance in all modes.  

   Powerful Image Analysis

 Label-free cell counting

Image analysis tools in Gen5 cover a very broad range of application requirements, and are both powerful and easy to use. Analysis functions in Gen5 extend to quantitative data as well. Cell count, confluence, signal translocation, subpopulation analysis and kinetic analysis are just some of the powerful data provided by Gen5’s image analysis tools.


   Publication-ready Images, Graphs and Data


Augmented Microscopy tools include the ability to create publication-ready images, graphs and data using the functions in Gen5 software. There is no need to export images or data to external software to create scatterplots, histograms, EC50 graphs and other data displays for publication.

Automated Image Capture



Image Prime
Basic Features
Supports all imaging instrument hardware functionality
Image Acquisition Modes and Features
Image up to 4 color channels per image set
Fast kinetic imaging (up to 10 fps)
Long-term kinetic imaging (up to 7 days)
Z-stack imaging of up to 50 slices for thick samples
Montage imaging for large samples
Combined z-stack and montage imaging
Set beacons (imaging regions of interest) per well or vessel
Image Processing
Background flattening to improve image signal to noise  
Add user defined image annotations to any image or graph  
Record movies of live samples and/or make movies of kinetic image series  
Image deconvolution to improve resolution  
Digital phase contrast to improve contrast in brightfield images  
Cellular Analysis      
Image Statistics (e.g., image total intensity, intensity above/below a threshold)
Cell count and confluence on a live sample
Cell count and confluence on an acquired image
Cellular analysis using a single analysis mask (e.g., object size, shape, area, circularity, intensity)  
Analyze subpopulations of cells expressing certain criteria (e.g., size, shape, intensity)  
Two analysis masks for advanced measurements (e.g., cytoplasmic signal, signal translocation)    
Advanced cellular analysis optimization tools    
Scatterplots/histograms for visualizing and gating cell level data    
Optional: Spot Counting Module for intracellular objects (e.g., mitochondria, steatosis)    

*For laboratories that require compliance to 21 CFR Part 11, Gen5 Secure editions are available
** For laboratories that require IVD compliance Gen5 IVD editions are available