Lionheart LX Automated Microscope

Cell Imaging & Microscopy > Automated Cell Imagers

Lionheart LX Automated Microscope


BioTek’s new Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.




   Automated digital microscopy

 Automated digital microscopy to 100x

Lionheart LX has an automated 6-objective turret and can contain up to 4 LED/filter cubes for multi-channel image capture. The precise automated stage and autofocus, auto exposure and other Gen5 software features enable simple, automated image capture and analysis.


Multiple imaging modes and processes


With brightfield, color brightfield and fluorescence imaging in four channels – and more than twenty available filter/LED cubes, Lionheart LX offers fantastic versatility that isn’t limited to image capture. Imaging processes from z-stacking to z-projection, montage collection to stitching; plus analysis from label-free cell counting to mitochrondrial membrane potential are all accomplished with Lionheart LX and Gen5 software.


   Easy imaging workflow automation

 easy imaging

BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.


   Instant cell count and confluence measurement


Get cell counts and confluence calculations directly on the live camera feed. Gen5 software controls Lionheart LX to quickly find the region of interest and display counts onscreen without needing to capture the image. The quick analyze function works for samples in a variety of labware.


   More than 20 filter/LED color cubes

Four imaging modes, multiple imaging processes

BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.


   Quick installation and easy setup


Lionheart LX integrates all microscopy hardware into a very compact footprint, saving valuable bench space. The easily accessible objectives and LED/filter cubes make setup and operation fast and simple, with a minimal learning curve.

Lionheart LX dimensions


Lionheart LX Automated Microscope. Fluorescence, high contrast brightfield and color brightfield imaging. Includes standard Gen5 software and 4-position slide holder. The imaging controller, objectives, filter/LED cubes, accessories and software upgrades are sold separately.


Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Software Gen5 Microplate Reader and Imager Software included
Gen5 Image+ and Image Prime Software available for advanced image analysis (option)
Gen5 Secure for 21 CFR Part 11 Compliance (option)
X/Y Stage resolution Lead screw driven stage with 0.1 micron resolution
Imaging - Inverted Microscope
Imaging mode Fluorescence, high contrast brightfield and color brightfield
Imaging method Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit grayscale Sony CMOS
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives
Objectives available Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
High NA: 20x, NA 0.75; 40x, NA 0.95
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller
Physical Characteristics
Power 60 Watts maximum consumption.
Dimensions 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
Weight 51 lbs (23.1 kg)
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.