Gen5 Software Features for Detection


Gen5 Software Features for Detection


Gen5™ offers powerful functionality for microplate reading and data analysis, enabling applications from micro-volume nucleic acid quantification to long-term cell-based kinetic assays.

Fluorophore spectra utility
The fluorophore spectra utility in Gen5 facilitates filter selection

Gen5 Software offers a unique combination of power and ease ­of ­use that drives productivity and saves time across all imaging and detection workflows.


Gen5 for Microplate Reading & Data Analysis

Images and data

Gen5™ offers powerful functionality microplate reading, enabling applications from micro-volume nucleic acid quantification to long-term cell-based kinetic assays.

  • Fluorophore spectra utility offers > 100 fluorophore spectra, making filter cube matching quick and easy
  • Enhanced conditional logic adds powerful functionality for data transformation, cutoffs and validation with automated outlier rejection and expanded formula operators
  • Improved file size, sorting and search tools for working with Gen5’s database and sample file database

Integrates with all BioTek detection systems


Gen5 Microplate Reader and Imaging Software is an integrated tool for data collection and analysis, compatible with all of BioTek’s multi-mode and single mode readers. It’s easy to get started with more than 150 prepared protocols within Gen5 and online. Gen5 supports endpoint, kinetic, well area scanning, spectral scanning read modes. One-button export sends your data right to Excel®, without requiring additional steps.

Synergy HTX and Gen5


Gen5 Secure: Features for 21 CFR Part 11 Compliance

Confirm and protect your results for GxP compliance

To meet GxP laboratory requirements Gen5 Secure offers features designed to meet 21 CFR Part 11 rules. Gen5 Secure is available for multi-mode and imaging application – all include: Built-in administration with no additional software required, multi-level user permissions, electronic signature and protocol and data audit trails with configurable alerts.


Powerful data analysis and flexible output options


Gen5 offers extensive data analysis tools for quantitative and qualitative analysis, including 4- and 5-parameter curve fits, EC/IC50 and Z’ calculations. Data reduction transformations and customizable validation and cutoff calculations, are all easily defined within the protocol. Gen5 provides quick low volume results with the Take3 plates, and data from any screen is exportable to Excel® at the touch of a single button.

Gather and analyze data from all BioTek microplate readers


QC and Validation Tools

QC and Validation Tools

Gen5 is fully validated at BioTek, click here to see the certificate. A Validation Package is available, providing an efficient, step-by-step approach to facilitate your internal validation. The Gen5 Validation Package is compatible with BioTek’s microplate reader Product Qualification Packages (IQ/OQ/PQ). Gen5 IVD and Gen5 IVD Image+ have QC Trending capability, security features and validation protocols for convenience and efficiency.


Gen5 is available in a several editions to meet the requirements of most laboratories. See a detailed feature comparison here.

Complete reader control and data analysis for all BioTek microplate readers; basic image analysis with Lionheart FX and Cytation

Reader Control only, for all BioTek microplate readers

Gen5 Secure
Complete reader control and data analysis for all BioTek microplate readers. Gen5 Secure includes features designed to help laboratories maintain critical GxP requirements including 21 CFR Part 11

Gen5 IVD
Complete reader control and data analysis for all BioTek microplate readers, with convenient QC Trending module and Validation Package included. Gen5 IVD is ideal for the clinical laboratory. Includes features to support 21CFR Part 11 compliance.


Recommended Computer System Requirements

For General Multi-Mode Detection Applications.

  • Windows 10* (Professional editions), Windows 8.1 (Professional editions), Windows 7 (Professional editions. Requires Service Pack 1 for Windows 7).
    • Gen5 3.0 requires 64-bit OS**
    • Gen5 2.09 and lower requires 32- or 64-bit OS.
  • Intel Core i5 or higher processor
  • 2G RAM or greater
  • 2 GB hard drive space
  • Monitor resolution 1280 x 800*** Keyboard/mouse
  • Software:
  • Microsoft Internet Explorer v 9.0 or later (for online Help)
  • Microsoft Excel for Quick Export and PowerExport:
    Gen5 3.0: Excel 2007 (32-bit edition only) or Excel 2010 - 2016 (32- and 64-bit editions)
    Gen5 2.0: Excel 2007 - 2016 (32-bit editions only)
  • Serial or USB 2.0 or 3.0 port for BioTek instrument connection
  • USB port for software installation

*Windows 10 N and Windows 10 KN require the Media Feature Pack for proper Gen5 operation.

**For Cytation customers who may plan to upgrade to imaging, a 64-bit OS is required for the USB 3.0 camera.

*** Gen5 is not designed for use on very high resolution monitors, e.g. 4K or 5K monitors