Gen5 Microplate Reader and Imager Software

Software & Robotics > Software

Gen5 Microplate Reader and Imager Software

Overview:

New! Gen5™ 3.03 offers new functionality for imaging and microplate reading, including spot counting for powerful cellular image analysis and a fluorophore spectra utility for convenience during fluorescence detection protocol definition, and many others.

 
Spot Counting
Spot Counting
Fluorophore spectra utility
Fluorophore spectra utility
 

From high magnification, intracellular image analysis to basic ELISA, Gen5 software helps transform raw data and images into meaningful results. Gen5 Software offers a unique combination of power and ease ­of ­use that drives productivity and saves time across all imaging and detection workflows.
 

Features:

New in Gen5 3.03 for Imaging & Microscopy

 
Deconvolution

Gen5 3.03 includes many new features that enhance image capture, processing, analysis and publication preparation, including:

  • Spot counting for detailed analysis of small intracellular objects or “spots”
  • Deconvolution processing step improves image resolution for better visualization of details
  • Scatterplots and histograms for cell-level data provide visual cues of trends in data, object subpopulations and outliers

New in Gen5 3.03 for Microplate Reading & Data Analysis

 
Images and data

New features in Gen5 3.03 enhance multi-mode data collection and analysis, with powerful functionality including

  • Fluorophore spectra utility offers > 100 fluorophore spectra, making filter cube matching quick and easy
  • Enhanced conditional logic adds powerful functionality for data transformation, cutoffs and validation with automated outlier rejection and expanded formula operators
  • Improved file size, sorting and search tools for working with Gen5’s database and sample file database

Integrates with all BioTek imaging and detection systems

 
Images and data

Gen5 Microplate Reader and Imaging Software is an integrated tool for data and image collection and analysis, compatible with all of BioTek’s imaging, multi-mode and absorbance readers. It’s easy to get started with more than 150 prepared protocols within Gen5 and online. Gen5 supports endpoint, kinetic, well area scanning, spectral scanning read modes. One-button export sends your data right to Excel®, without requiring additional steps.
 

Gen5 Image Prime: Advanced image analysis tools

 
Explore, image and analyze intra-and inter-cellular activity

With Lionheart FX and Cytation imaging systems, Gen5 supports Augmented Microscopy™, the automated workflow incorporating image capture, processing, analysis and preparation of publication-ready images and data. Gen5 Image Prime takes image analysis to advance levels including powerful analysis tools like with nuclear mask expansion and reduction, secondary mask for cytoplasm or whole cell identification, custom object-level measurements for translocation and subpopulation analysis.


Gen5 Secure: Features for 21 CFR Part 11 Compliance

 
Confirm and protect your results for GxP compliance

To meet GxP laboratory requirements Gen5 Secure offers features designed to meet 21 CFR Part 11 rules. Gen5 Secure is available for multi-mode and imaging application – all include: Built-in administration with no additional software required, multi-level user permissions, electronic signature and protocol and data audit trails with configurable alerts.


Powerful data analysis and flexible output options

 
Gather and analyze data from all BioTek microplate readers

Gen5 offers extensive data analysis tools for quantitative and qualitative analysis, including 4- and 5-parameter curve fits, EC/IC50 and Z’ calculations Data reduction transformations and customizable validation and cutoff calculations, are all easily defined within the protocol. Gen5 provides quick low volume results with the Take3 plates, and data from any screen is exportable to Excel® at the touch of a single button.

 

QC and Validation Tools

 
QC and Validation Tools

Gen5 is fully validated at BioTek, click here to see the certificate. A Validation Package is available, providing an efficient, step-by-step approach to facilitate your internal validation. The Gen5 Validation Package is compatible with BioTek’s microplate reader Product Qualification Packages (IQ/OQ/PQ). Gen5 IVD and Gen5 IVD Image+ have QC Trending capability, security features and validation protocols for convenience and efficiency.
 

Imaging: Methods and Processes

 
Explore, image and analyze intra-and inter-cellular activity

Gen5 offers powerful imaging methods and processing, including high speed imaging and image binning for fast kinetics, z-stacking and z-projections, montage capture and stitching, time-lapse movie making with automated image adjustment and much more. Several editions are available to meet your laboratory’s imaging requirements.


Explore, image and analyze intra-and inter-cellular activity

 
Gather and analyze data from all BioTek microplate readers

Captures high quality images from 1.25x to 100x with Lionheart FX, up to 60x with Cytation and Gen5. Combine multiple image acquisition steps and multi-mode detection steps to obtain and analyze both phenotypic and quantitative data. Image file from other programs can be imported and analyzed in Gen5.


Models:

Gen5 is available in a several editions to meet the requirements of most laboratories. See a detailed feature comparison here.

Gen5
Complete reader control and data analysis for all BioTek microplate readers; basic image analysis with Lionheart FX and Cytation

Gen5RC
Reader Control only, for all BioTek microplate readers

Gen5 Image+
Gen5™ Image+ software offers complete imaging control for Lionheart FX Automated Live Cell Imager and Cytation Cell Imaging Multi¬-Mode Readers. Gen5 Image+ includes image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.

Gen5 Secure
Complete reader control and data analysis for all BioTek microplate readers. Gen5 Secure includes features designed to help laboratories maintain critical GxP requirements including 21 CFR Part 11                       

Gen5 Secure Image+
Offers features and functionality found in both Gen5 Image+ and Gen5 Secure

Gen5 IVD
Complete reader control and data analysis for all BioTek microplate readers, with convenient QC Trending module and Validation Package included. Gen5 IVD is ideal for the clinical laboratory

Gen5 IVD Image+
Offers features found in both Gen5 IVD and Gen5 Image+

Gen5 Image Prime*
Gen5™ Image Prime Software offers imaging control for Lionheart FX Automated Live Cell Imager and Cytation Cell Imaging Multi-Mode Readers. Gen5 Image Prime includes image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.

Gen5 Secure Image Prime*
Offers features and functionality found in both Gen5 Image Prime and Gen5 Secure.

*Gen5 Image Prime and Gen5 Secure Image Prime are for Research Use Only. Not for Diagnostic Use.


Specifications:

General
Recommended Computer System Requirements

For Imaging Applications (Lionheart FX, Cytation 5 and Cytation 3)

  • 64-bit version of Windows 7 or Windows 10 Professional
  • Intel® Core i5 (4th generation) Processor or higher
  • Intel® 8 USB Chipset or higher
  • 8 GB RAM or greater
  • 128 GB Hard Drive space or greater*
  • Monitor resolution of 1600 x 900**
  • Software:
    (Lionheart FX requires Gen5 3.0 or higher)
  • Microsoft Internet Explorer v 9.0 or later (for online Help)
  • Microsoft Excel for Quick Export and PowerExport:
    Gen5 3.0: Excel 2007 (32-bit edition only) or Excel 2010 - 2016 (32- and 64-bit editions) 
    Gen5 2.0: Excel 2007 - 2013 (32-bit editions only)
  • Keyboard and mouse
  • Connectivity:
  • USB 3 port for camera
  • USB 2 or 3 port for instrument

*Each image is approximately 2MB in size. Image file management is the responsibility of the user. For best performance, use an RJ-45 Lan connector for network use. Do not connect to a network using WiFi.
**Gen5 is not designed for use on very high resolution monitors, e.g. 4K or 5K monitors.

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For General Multi-Mode Detection Applications.

  • Windows 10, Windows 8.1, Windows 7 (Professional editions). Gen5 3.0 requires 64-bit OS,
    Gen5 2.09 and lower requires 32- or 64-bit OS.
  • Intel Core i5 or higher
  • 2G RAM or greater
  • 2 GB hard drive space
  • Monitor resolution 1280 x 800** Keyboard/mouse
  • Software: 
  • Microsoft Internet Explorer v 9.0 or later (for online Help)
  • Microsoft Excel for Quick Export and PowerExport:
    Gen5 3.0: Excel 2007 (32-bit edition only) or Excel 2010 - 2016 (32- and 64-bit editions)
    Gen5 2.0: Excel 2007 - 2013 (32-bit editions only)
  • Serial or USB 2.0 or 3.0 port for BioTek instrument connection
  • USB port for software installation

*For Cytation customers who may plan to upgrade to imaging, a 64-bit OS is required for the USB 3.0 camera.
** Gen5 is not designed for use on very high resolution monitors, e.g. 4K or 5K monitors