BioSpa Live Cell Imaging System

Cell Imaging & Microscopy > Automated Cell Imagers

BioSpa Live Cell Imaging System


The BioSpa Live Cell Imaging System integrates BioSpa Automated Incubator and Cytation 5 Cell Imaging Multi-Mode Reader to fully automate kinetic live cell imaging and analysis. Plate washing and reagent dispensing can be added to the system for complete walk-away automation, from sample prep through image analysis.


Unattended workflow automation

Unattended workflow automation
BioSpa 8 handles up to 8 microplates, Petri and cell culture dishes and T25 flasks during automated processes, running up to 2 weeks. Place the labware in BioSpa’s drawers, and the gripper moves to the connected imaging, detection and liquid handlers as scheduled in the BioSpa software.


Environmental controls for live cell assays

Integrated Temp
Critical physiological conditions are maintained in BioSpa: Incubation to 45 °C, CO2/O2 control and humidity monitoring facilitate live cell processes. BioSpa software continuously records the environment conditions to ensure assay success. Customizable text and email notifications allow you to walk away from the process with confidence.


Fluorescence, high contrast brightfield, color brightfield and phase contrast imaging

Label-free cell counting
BioSpa 8 integration with Cytation 5 brings powerful image collection and analysis tools to the automated workflow. With six turret positions for objectives ranging from 1.25x to 60x, four channels plus brightfield, and more than 15 colors available, Cytation 5 brings unsurpassed capabilities to automated digital microscopy for many long-term live cell imaging processes.


OnDemand Mode

BioSpa on demand
For simple, efficient imaging or detection protocols, OnDemand mode allows labware to be added or removed at any time, without interrupting operations in process. Multiple users can be defined in the system and each user can visually track their own experiment’s progress on the dynamic timeline.


Advanced cell-level analysis with Gen5 Software

Kinetic live cell assay support
Gen5 Microplate Reader and Imager software is available in several editions to meet the imaging and analysis requirements of many applications. From basic automated cell counting to advance capabilities including dual masks for nucleus and cytoplasm identification, Gen5 offers complete and powerful quantitative data analysis in and uncomplicated interface.


Washing and media replacement

Washing and media replacement
BioSpa 8 is compatible with BioTek’s MultiFlo FX and EL406 for plate washing and media exchange. Adding a combination washer dispenser or washer to the BioSpa 8 and Cytation integrated system provides complete automated process from sample prep through image analysis.
  • Up to 60x magnification and 4 color channels covers a range of applications
  • Single- and multi-color, montage, time lapse and automated z-stacking
  • Powerful image processing: z-projection, digital phase contrast and image stitching
  • Compact system fits in biosafety cabinet for the ultimate cell assay protection
  • Lidded plate handling, easy maintenance


BioSpa Live Cell Imaging system can be comprised of of BioSpa 8 Automated Incubator, along with other compatible imagers, washers and dispensers:

BioSpa System

For imaging- or detection-only workflows using BioSpa OnDemand mode, Cytation and Synergy Neo2 can be integrated on the right or the left side of BioSpa. Below is the left-side integration.

BIoSpa and Cytation


Microplate types 6- to 1536-well standard height microplates, with or without lids.
Plate height range: 7.6 mm to 25.4 mm
Other labware supported Cell culture dishes (60 mm and 35 mm), T25 flasks
Lidded plate handling Robotic arm moves plate to and from BioSpa 8 and connected instrument; handles de-lidding and re-lidding plates.
Microplate capacity Up to 8 microplates (or other labware)
Air filter User-replaceable HEPA filter
Compatible instruments Cytation 5, Cytation 3, Cytation 1, Synergy Neo2, Synergy H1, Epoch 2, EL406, 405 TS, 405 LS, MultiFlo FX, MultiFlo
Temperature control Range: to 45 °C
Control resolution: 0.1 °C
Uniformity + 0.5 °C at 37 °C
Software BioSpa Automated Incubator Software:

Provides programming interface for BioTek detection and liquid handling devices
Allows user notification (text or email) of events and/or errors in the system
Provides control, monitoring and logging of:
    incubator temperature
Provides humidity level monitoring and logging
Allows uninterrupted runs lasting days or weeks
BioSpa Session mode for all imaging, detection and liquid handling operations
BioSpa OnDemand mode for simplified, intuitive imaging- or detection-only workflows for single or multiple users
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2+0.2% at 1% O2
Humidity control rH: 80 to 95% (lidded plates and 5% CO2)
Source: Removable water pan
Water level sensor: Low water level alert
Imaging System
Imaging mode Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit gray scale, Sony CCD, 1.25 megapixel. 0.3 µm/pixel at 20x
Objective capacity 6 user-replaceable objectives
Objectives available 1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions User-trained autofocus, autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Liquid handling See the individual product pages at EL406, MultiFlo FX and 405 TS