BioSpa Live Cell Analysis System

Cell Imaging & Microscopy > Multi-Plate Live Cell Imaging System

BioSpa Live Cell Analysis System


Capture detailed cellular images and get in-depth cell-level analyses for migration and wound healing assays, 3D spheroid formation and many other kinetic applications in up to 8 microplates or other labware. Built-in scheduling, environmental monitoring and available liquid handling allow you to walk away with confidence – and allows multiple users to run processes simultaneously without disrupting others.

The BioSpa Live Cell Analysis System offers unique benefits for a wide variety of live cell imaging applications:

Fluorescent images of fibroblast



   Five imaging modes

 Automated digital microscopy to 100x

The variety of imaging modes enable an astonishing range of microscopy applications, within a single, compact system. Automate workflows for up to 8 microplates or other labware, including cell culture dishes, flasks and microscope slides.

   Broad magnification range


Capture the level of detail you need, from whole well montage to intracellular detail with 1.25x to 60x objectives available. The automated, 6-position turret makes it simple to capture multiple magnifications in a single workflow.

Four imaging modes, multiple imaging processes

   Environmental controls


Successful long term live cell assays rely on a controlled environment to keep cells healthy and aid proliferation. BioSpa has integrated temperature and CO2/O2 control and monitoring, plus humidity monitoring to protect cells during kinetic runs that last days or weeks.


   Automated objective turret


To efficiently view a variety of samples at a range of magnifications, install up to 6 objectives on the automated turret, and capture images automatically, within a single experiment.


   3D cell culture

3D cell culture

Gen5 software has powerful z-stacking capabilities to capture the important details of a 3D cell structure. Z-projection and image analysis provides in-depth information about the spheroid. Z-stacking montage capability brings powerful processing for 3D biologies.


   Patented laser autofocus


Provides increased reproducibility and speed, while decreasing phototoxic and photobleaching effect. Learn more about Laser Autofocus on the Technology Page.

Label-free cell counting

   Advanced cell-level analysis


Powerful tools like nuclear and cytoplasmic masking define critical, information-rich regions of interest for automated, detailed cellular analysis.


Compact and Efficient Design


The BioSpa Live Cell Analysis System easily fits inside commercially available containment enclosures and biosafety cabinets. The Baker Company offers a broad range of innovative solutions to provide isolation of cell lines and containment of potentially hazardous agents. Fluidics, power requirements, heat rejection, service and user interface ergonomics can all be accommodated.  BioTek and Baker continually work together to provide automated life science laboratories with the best possible solution. 

Compact and Efficient Design


BioSpa Live Cell Analysis System is typically comprised of Cytation Cell Imaging Multi-Mode Reader and BioSpa 8 Automated Incubator. BioTek’s washers and dispensers can be integrated for a fully automated sample prep through results workflow. Below are the BioTek instruments that can be integrated with BioSpa for automated live cell imaging.

BioSpa System

Cytation can be integrated on the right or left side of BioSpa, as shown below.

BIoSpa and Cytation


Microplate types 6- to 1536-well standard height microplates, with or without lids.
Plate height range: 7.6 mm to 25.4 mm
Other labware supported Cell culture dishes (60 mm and 35 mm)
Lidded plate handling BioSpa automated plate movement, including de-lidding and re-lidding between the incubator and imager.
Microplate capacity Up to 8 microplates (or other labware)
Air filter User-replaceable HEPA filter
Compatible instruments Cytation 7, Cytation 5, Cytation 1, EL406, 405 TS, MultiFlo FX
Temperature control Range: to 45 °C
Control resolution: 0.1 °C
Uniformity + 0.5 °C at 37 °C
Software Allows user notification (text or email) of events and/or errors in the system
Provides control, monitoring and logging of CO2/O2 and incubator temperature
Provides humidity level monitoring and logging
BioSpa OnDemand mode for simplified, intuitive workflows for single or multiple users
BioSpa Session mode for all imaging, detection and liquid handling operations
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2+0.2% at 1% O2
Humidity control rH: 80 to 95% (lidded plates and 5% CO2)
Source: Removable water pan
Water level sensor: Low water level alert
Imaging - Inverted Microscope
Imaging mode Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit gray scale Sony
Objective capacity 6 user-replaceable objectives
Objectives available 1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available More than 20 colors are available
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions User-trained autofocus, autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Liquid handling See the individual product pages at EL406, MultiFlo FX and 405 TS