BioSpa Live Cell Imaging System

Imaging & Microscopy > Automated Cell Imagers

BioSpa Live Cell Imaging System

Overview:

The BioSpa Live Cell Imaging System integrates BioSpa Automated Incubator and Cytation 5 Cell Imaging Multi-Mode Reader to fully automate kinetic live cell imaging and analysis. Plate washing and reagent dispensing can be added to the system for complete walk-away automation, from sample prep through image analysis.
 

Features:

Unattended workflow automation

 
Unattended workflow automation
BioSpa 8 handles up to 8 microplates, Petri and cell culture dishes and T25 flasks during automated processes, running up to 2 weeks. Place the labware in BioSpa’s drawers, and the gripper moves to the connected imaging, detection and liquid handlers as scheduled in the BioSpa software.

 

Environmental controls for live cell assays

Integrated Temp
Critical physiological conditions are maintained in BioSpa: Incubation to 45 °C, CO2/O2 control and humidity monitoring facilitate live cell processes. BioSpa software continuously records the environment conditions to ensure assay success. Customizable text and email notifications allow you to walk away from the process with confidence.

 

Fluorescence, high contrast brightfield, color brightfield and phase contrast imaging

Label-free cell counting
BioSpa 8 integration with Cytation 5 brings powerful image collection and analysis tools to the automated workflow. With six turret positions for objectives ranging from 1.25x to 60x, four channels plus brightfield, and more than 15 colors available, Cytation 5 brings unsurpassed capabilities to automated digital microscopy for many long-term live cell imaging processes.

 

Advanced cell-level analysis with Gen5 Software

 
Kinetic live cell assay support
Gen5 Microplate Reader and Imager software is available in several editions to meet the imaging and analysis requirements of many applications. From basic automated cell counting to advance capabilities including dual masks for nucleus and cytoplasm identification, Gen5 offers complete and powerful quantitative data analysis in and uncomplicated interface.

 

Washing and media replacement

Washing and media replacement
BioSpa 8 is compatible with BioTek’s MultiFlo FX and EL406 for plate washing and media exchange. Adding a combination washer dispenser or washer to the BioSpa 8 and Cytation integrated system provides complete automated process from sample prep through image analysis.
 

Models:

BioSpa Live Cell Imaging system can be comprised of of BioSpa 8 Automated Incubator, along with other compatible imagers, washers and dispensers:



BioSpa System

Specifications:

General
Microplate types 6- to 1536-well standard height microplates, with or without lids.
Plate height range: 7.6 mm to 25.4 mm
Other labware supported Cell culture dishes (60 mm and 35 mm), T25 flasks
Lidded plate handling Robotic arm moves plate to and from BioSpa 8 and connected instrument; handles de-lidding and re-lidding plates.
Microplate capacity Up to 8 microplates (or other labware)
Air filter User-replaceable HEPA filter
Compatible instruments Cytation 5, Cytation 3, Synergy Neo2, Synergy H1, Epoch 2, EL406, 405 TS, 405 LS, MultiFlo FX, MultiFlo
Temperature control Range: to 45 °C
Control resolution: 0.1 °C
Uniformity + 0.5 °C at 37 °C
Software BioSpa Automated Incubator Software:

Provides programming interface for BioTek detection and liquid handling devices
Allows user notification (text or email) of events and/or errors in the system
Provides control, monitoring and logging of:
    CO2/O2
    incubator temperature
Provides humidity level monitoring and logging
Allows long-term uninterrupted runs up to 2 weeks
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2+0.2% at 1% O2
Humidity control rH: 80 to 95% (lidded plates and 5% CO2)
Source: Removable water pan
Water level sensor: Low water level alert
Imaging System
Imaging mode Fluorescence
Brightfield
Color Brightfield
Phase Contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit gray scale, Sony CCD, 1.25 megapixel. 0.3 µm/pixel at 20x
Objective capacity 6 user-replaceable objectives
Objectives available 1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions User-trained autofocus, autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Liquid handling See the individual product pages at EL406, MultiFlo FX and 405 TS