Lionheart FX Automated Microscope

Cell Imaging & Microscopy > Automated Cell Imagers

Lionheart FX Automated Microscope


The Agilent BioTek Lionheart FX automated microscope is a compact system for a broad range of imaging workflows. It offers up to 60x air, in addition to 60x and 100x oil immersion magnification. Combined with fluorescence, brightfield, color brightfield, and phase contrast imaging channels, the imager provides maximum application reach. An optional environment control cover provides incubation to 40 °C, in addition to effective containment for CO2/O2 control to enable performance of cell-based assays. A humidity chamber can be added to optimize conditions for long-term live cell imaging applications. An available dual reagent injector also facilitates rapid kinetic assays. 

Click here to view our Image Gallery of images captured with the Lionheart FX.


green box Small benchtop footprint

Small benchtop footprint

The Lionheart FX has a small footprint and is easily installed on a standard lab bench with no need for a dedicated darkroom.

green box High-quality optical components

High-quality optical components

Lionheart FX uses high-quality Olympus objectives and Semrock filters, which drive excellent image quality and performance.

green box Wide field of view

Wide field of view

The wide field of view (WFOV) camera provides fast, automated imaging in microplates and slides. Large tissue sections (left) and microplates (right) can be imaged from low to high magnifications. 

green box Augmented microscopy

Augmented microscopy

When paired with Gen5 imaging software, the Lionheart FX provides a unique fully automated capture, process, analyze and publish workflow.

green box Environmental controls for live cell imaging 

 Environmental controls for live cell imaging 

Successful live cell kinetic imaging relies on a consistent environment, including temperature, CO2/O2, and humidity control. Lionheart FX provides the perfect environment to grow and analyze live cells over time. Powerful movie maker and kinetic analysis software tools allow visualization and analysis of time-lapse experiments.

green box Open-stage design

Open-stage design

The open design of the Lionheart FX stage allows researchers to work with microfluidic devices.

green box Autofocus customized for your sample types and labware

Autofocus customized for your sample types and labware

A. Image-based autofocus is available on all Agilent BioTek imaging systems. It focuses on the plane of highest contrast in the sample, including “shifting” biology within the well.
B. The BioTek patented autofocus method uses the same focal offset from a laser identified interface unique to each well or area to be imaged. It is typically faster, works with dim fluorophores, and helps prevent phototoxicity and photobleaching. Laser autofocus also offers better reproducibility and higher accuracy during long term kinetic imaging.


green box Dual-reagent injector module

 Dual-reagent injector module

The dual-reagent injector module for Lionheart FX allows fast cellular reactions to be imaged after the addition of a reagent.

For Research Use Only. Not for use in diagnostic procedures.


Lionheart FX Automated Live Cell Imager. Fluorescence, brightfield, color brightfield and phase contrast imaging capabililty. Includes base Gen5 software. Objectives, filter/LED cubes, accessories and software upgrades sold separately.


Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Temperature control To 40 °C; uniformity +/- 0.5 °C at 37 °C
Software Gen5 Microplate Reader and Imager Software (included)
Optional software:
Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure, Gen5 Secure Image+, Gen5 Secure Image Prime: 21 CFR Part 11 compliant features
Auto ROI module
Spot Count module
Cell Count & Viability app
CO2 and O2 control (option) Range: 0 - 20% (CO2)
1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% (CO2)
+0.2% at 1% (O2)
Models for both CO2/O2 or CO2 only are available
Environmental control cover Top cover for light tight imaging and incubation control (option)
X/Y Stage resolution Lead screw driven stage with 0.1 µm resolution
Humidity control Humidity chamber with rapid gas recharge (option)
Imaging - Field of view (WFOV) mode 3.42 x 3.42 mm at 4x magnification
Imaging - Widefield Microscope
Imaging mode Fluorescence, contrast brightfield, high contrast brightfield, color brightfield, and phase contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking, z-stack montage
Light source User-replaceable LEDs (available wavelengths: 365 nm, 390 nm, 405nm 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Image processing Z-projection, digital phase contrast, stitching
Camera Sony CMOS 16-bit grayscale camera
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
Movies: MP4, WMV
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available Air: 1.25x, 2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Oil: 60x, 100x
Phase objectives available 4x, 10x, 20x, 40x      
Image filter cube capacity 4 user-replaceable fluorescence cubes
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller
Image flatness WFOV mode <20% CV for blank uncorrected brightfield images
<5% CV for corrected brightfield images, when using > 4X magnification
Image capture rate Laser autofocus, 20x, 100 ms exposure, 96 wells: 4 min, 4 sec
Software autofocus, 20x, 100 ms exposure, 96 wells: 7 min, 3 sec
Reagent Injectors (option)
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume <1.65 mL with back flush
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays
Offset tip - dispensing is offset from the optical path
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Physical Characteristics
Power External 250W (minimum), 24VDC power supply compatible with 100-240 VAC @50-60Hz.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)
Weight With top cover: 58 lbs (26.3 kg)
Without top cover: 51 lbs (23.1kg)
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.