BioSpa 8 Automated Incubator


BioSpa 8 Automated Incubator


BioSpa 8 automated incubator links Agilent BioTek readers and imagers together with washers and dispensers. This integration provides a full workflow automation of up to 8 microplates or other labware. BioSpa 8 can automate your workflows, from ELISA to long-term live cell assays.;Temperature, CO2/O2 control, humidity monitoring, and lid handling ensure an ideal environment for cell cultures. BioSpa software records session timelines and environmental conditions and sends text and email alerts during long and short-term runs. Link a washer, dispenser, plate reader, or imager for complete workflow automation.



Complete workflow automation, sample prep to detection

BioSpa 8

BioSpa8 handles up to 8 microplates, Petri and cell culture dishes during automated processes, running for days to weeks. Place the labware in BioSpa’s drawers, and the gripper moves to the connected imaging, detection and liquid handlers as scheduled in the BioSpa software.

Temperature, CO2/O2 control; humidity monitoring


Critical physiological conditions are maintained in BioSpa: Incubation to 45 °C, CO2/O2 control and humidity monitoring facilitate live cell processes. BioSpa software continuously records the environment conditions to ensure assay success.

Integrated temp


Records environment conditions; automated alerts

environment conditions

BioSpa software monitors and records the system and environment conditions throughout the entire runtime. Customizable text and email notifications allow you to walk away from the process with confidence.


OnDemand Mode

For simple, efficient imaging or detection protocols, OnDemand mode allows labware to be added or removed at any time, without interrupting operations in process. Multiple users can be defined in the system and each user can visually track their own experiment’s progress on the dynamic timeline.
BioSpa on demand



Compact for benchtop use, or for optional biosafety cabinet use

BioSpa in the hood

When the workflow calls for sterile conditions, BioSpa and integrated instruments is compact enough to fit in a biosafety enclosure.

Uncomplicated software and simple integration


BioSpa software integrates seamlessly with Gen5 and LHC software to provide easy and intuitive scheduling for short and long-term processes.

Washing media


Automated incubator with 8-plate capacity, incubation to 45 °C. Includes BioSpa software and one alignment kit.

Automated incubator with 8-plate capacity, incubation to 45 °C and CO2/O2 control and monitoring, water pan and humidity monitoring. Includes BioSpa software and one alignment kit.

BioSpa 8 can integrate to one washer or dispenser, one imager or reader, or to two instruments for complete, unattended workflow automation.


Microplate types 6- to 1536-well standard height microplates, with or without lids.
Plate height range: 7.6 mm to 25.4 mm
Other labware supported Cell culture dishes (60 mm and 35 mm)
Lidded plate handling Robotic arm moves plate to and from BioSpa 8 and connected instrument; handles de-lidding and re-lidding plates.
Microplate capacity Up to 8 microplates (or other labware)
Air filter User-replaceable HEPA filter
Compatible instruments Cytation C10 (confocal and widefield), Cytation 7, Cytation 5, Cytation 1, Synergy Neo2, Synergy H1, Epoch 2, EL406, 405 TS, 405 LS, MultiFlo FX
Interfacing capacity 1 or 2 devices: Reader/imager only, washer/dispenser only, or both
Software BioSpa Automated Incubator Software:

Provides programming interface for BioTek detection and liquid handling devices
Allows user notification (text or email) of events and/or errors in the system
Provides control, monitoring and logging of:
  • CO2/O2
  • incubator temperature
  • Provides humidity level monitoring and logging
    Allows long-term uninterrupted runs
    CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
    Control Resolution: +0.1% (CO2 and O2)
    Stability: +0.2% at 5% CO2; +0.2% at 1% O2
    Temperature Control Range: to 45 °C
    Control resolution: 0.1 °C
    Uniformity: + 0.5 °C at 37 °C
    Humidity (option) rH: 80 to 95% (lidded plates and 5% CO2)
    Source: Removable water pan
    Water level sensor: Low water level alert
    Recommended Computer System Requirements
  • Intel Pentium P4 processor or compatible/ 1.33 GHz
  • Windows 7, Windows 8.1 or Windows 10 Professional Editions  
  • 2 GB of RAM
  • Designed for XGA Resolution 1080 x 860
  • 10 GB of available Hard Drive space
  • Keyboard and mouse; USB ports
  • Microsoft Internet Explorer v 9.0 or higher (for online Help)
  • LHC™ v 2.19 or higher for washer or dispenser integration
  • Gen5 2.09 or higher for reader or imager integration
  • Physical Characteristics
    Weight < 85 lbs (39 kg) (without shipping components and an empty water pan)
    Dimensions With standard base pan extension: 29.5" L x 22" W x 19"H (75 cm x 55 cm x 48 cm)
    With fluidics base pan extension: 39" L x 22" W x 19" H (99 cm x 55 cm x 48 cm)
    Regulatory CE and TUV marked, RoHS compliant.