Synergy HTX Multi-Mode Reader

Detection > Multi-Mode Microplate Readers

Overview:

Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. It’s unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence and AlphaScreen®/ AlphaLISA® workflows. Incubation and shaking plus a dual reagent injector module to meet all your laboratory’s assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 Software for data collection, analysis, exporting and reporting.

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Features:

Great flexibility at a low price

Affordability doesn’t mean compromising on performance with the Synergy HTX. Its hardware design and Gen5-driven functionality address a wide range of multi-mode applications without costly and often unused options.

Monochromator - based UV/Vis absorbance and filter-based fluorescence

Synergy HTX provides great sensitivity with its filter-based fluorescence optics, and the monochromator-based absorbance optics offers 200 nm – 999 nm measurements without requiring filters.

Linear and orbital shaking support and optimize many applications

Assays that require shaking, such as bacterial and yeast growth studies, will benefit from the robust, programmable shaking options in Synergy HTX.

Excellent performance with AlphaScreen® and AlphaLISA®

The sensitive fluorescence optics in Synergy HTX provide great performance for many applications including important Alpha assays.

Dual reagent injectors are ideal for inject/read assays

Optional dual reagent injector module with angled tips, for inject/read applications, such as enzyme kinetics and Dual-Luciferase® Reporter. Synergy HTX is easily upgradable to add the dual reagent injector.

Models:

Part #	S1L	S1A	S1LA	S1LF	S1LFA	S1LFTA
Absorbance		•	•		•	•
Fluorescence				•	•	•
Luminescence	•		•	•	•	•
Incubation	•	•	•	•	•	•
Shaking	•	•	•	•	•	•
Dual Reagent Injector*	•	•	•	•	•	•
TRF (secondary mode)						•
Software	Gen5™ included with all configurations

*Dual Reagent Injector module is optional, must be purchased separately

Specifications:

General
Detection modes	UV-Vis absorbance Fluorescence intensity Luminescence Time-resolved fluorescence (secondary mode) Alpha
Read methods	End point, kinetic, spectral scanning and well area scanning
Microplate types	6- to 384-well plates
Other labware supported	PCR plates, Petri and cell culture dishes Take3 Micro-Volume Plates
Temperature control	4-Zone™ incubation to 50 °C with Condensation Control™ +0.2 °C at 37 °C
Shaking	Linear, orbital
Software	Gen5™ Data Analysis Software Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation	BioStack and 3rd party automation compatible
Absorbance
Light source	Xenon flash
Detector	photodiode
Wavelength selection	monochromator
Wavelength range	200 - 999 nm, 1 nm increments
Monochromator bandwidth	2.4 nm
Dynamic range	0 - 4.0 OD
Resolution	0.0001 OD
Pathlength correction	yes
Monochromator wavelength accuracy	+2 nm
Monochromator wavelength repeatability	+0.2 nm
OD accuracy	<1% at 2.0 OD <3% at 3.0 OD
OD linearity	<1% from 0 to 3.0 OD
OD repeatability	<0.5% at 2.0 OD
Stray light	0.03% at 230 nm
Reading speed (kinetic)	96 wells: 14 seconds 384 wells: 26 seconds
Fluorescence Intensity
Light source	Tungsten halogen Xenon flash (option)
Detector	PMT
Wavelength selection	Filters
Wavelength range	300 - 700 nm (200 - 850 nm option)
Dynamic range	>6 decades
Sensitivity	Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate)
Reading speed (kinetic)	96 wells: 31 seconds 384 wells: 80 seconds
Luminescence
Wavelength range	300 - 700 nm
Dynamic range	>6 decades
Sensitivity	10 amol ATP (flash) - Lum and Abs / Lum configurations 30 amol ATP (flash) - Multi-mode configurations
Time-Resolved Fluorescence
Light source	Xenon flash
Detector	PMT
Wavelength selection	Monochromator
Alpha Detection
Light source	Tungsten halogen
Detector	PMT
Wavelength selection	Filters
Sensitivity	300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate
Read speed	2 minute (96-well plate)
Reagent Dispensers
Supported detection modes	All modes
Number	2 syringe pumps
Supported labware	6- to 384-well plates
Dead volume	1.1 mL with back flush
Dispense volume	5-1000 µL in 1 nm increments
Dispense accuracy	+1 µL or 2%
Dispense precision	<2% at 50 - 200 µL
Physical Characteristics
Connectivity	1 USB, 1 RS232 for external PC control
Power	100-240 VAC, 50/60 Hz
Dimensions	16"W x 15"D x 10"H (40.6 x 38 x 25.4 cm)
Weight	40 lbs (18 kg)
Regulatory
Regulatory	CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.