Synergy HTX Multi-Mode Reader

Microplate Readers: Multi-Mode and Absorbance Readers > Multi-Mode Microplate Readers

Synergy HTX Multi-Mode Reader


The BioTek Synergy HTX is a compact, economical multimode reader for 6- to 384-well microplates and Take3 microvolume plates. The unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence, and AlphaScreen/AlphaLISA workflows. With incubation and shaking, plus a dual reagent injector module, the system will meet all your assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 software for data collection, analysis, exporting, and reporting.



Great flexibility at a low price

Affordability doesn’t mean compromising on performance with the Synergy HTX. Its hardware design and Gen5-driven functionality address a wide range of multi-mode applications without costly and often unused options.


Monochromator and filter optics

Synergy HTX provides great sensitivity with its filter-based fluorescence optics, and the monochromator-based UV-Vis absorbance optics offers 200 nm – 999 nm measurements without requiring filters. The combination of independent optics allows multi-mode measurements without compromise.
Patented Technology


Shaking for assay optimization

Linear and orbital shaking
Assays that require shaking, such as bacterial and yeast growth studies, will benefit from the robust, programmable shaking options in Synergy HTX. Select linear or orbital shaking, with programmable speed and duration.

Excellent Alpha assay performance

The sensitive fluorescence optics in Synergy HTX provide great performance for many applications including important AlphaScreen and AlphaLISA assays.
AlphaScreen® and AlphaLISA®

Fast inject/read assays

Dual reagent injectors

The available dual reagent injector module with angled tips, enabels fast inject/read operations, which is critical for enzyme kinetics, Dual-Luciferase Reporter and many other assays. Synergy HTX is easily upgradable to add the dual reagent injector.


Dual Reagent Injector*
TRF (secondary mode)
Gen5 included with all configurations
*Dual Reagent Injector module is optional, must be purchased separately


Detection modes UV-Vis absorbance
Fluorescence intensity
Time-resolved fluorescence (secondary mode)
Read methods End point, kinetic, spectral scanning and well area scanning
Microplate types 6- to 384-well plates
Other labware supported PCR plates, Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone™ incubation to 50 °C with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 - 999 nm, 1 nm increments
Monochromator bandwidth 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 14 seconds
384 wells: 26 seconds
Fluorescence Intensity
Light source Tungsten halogen
Xenon flash (option)
Detector PMT
Wavelength selection Filters
Wavelength range 300 - 700 nm (200 - 850 nm option)
Dynamic range >6 decades
Sensitivity Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate)
Reading speed (kinetic) 96 wells: 31 seconds
384 wells: 80 seconds
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 10 amol ATP (flash) - Lum and Abs / Lum configurations
30 amol ATP (flash) - Multi-mode configurations
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Monochromator
Alpha Detection
Light source Tungsten halogen
Detector PMT
Wavelength selection Filters
Sensitivity 300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate
Read speed 2 minute (96-well plate)
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates
Dead volume 1.1 mL with back flush
Dispense volume 5-1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Physical Characteristics
Connectivity 1 USB, 1 RS232 for external PC control
Power 100-240 VAC, 50/60 Hz
Dimensions 16"W x 15"D x 10"H
(40.6 x 38 x 25.4 cm)
Weight 40 lbs (18 kg)
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.