Cytation Hybrid Multimode Reader

Microplate Readers: Multi-Mode and Absorbance Readers > Multi-Mode Microplate Readers

Cytation Hybrid Multimode Reader


The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. Detection modes are available in UV-Vis absorbance, fluorescence, and luminescence detection. Fluorescence microscopy to 60x can be added to expand the range of applications in a single instrument. Gen5 software is included with the system and is designed for uncomplicated data collection and processing for the most complex assays.



   Modular, upgradable design

 Digital microscopy and multi-mode detection

Although your lab may only be working with multimode workflows now, assay demands may change in the future. Cytation’s modularity offers the ability to add automated digital microscopy to the multi-mode system to adapt as your research requirements change.


Patented Hybrid optics


Cytation 5 features a patented, hybrid optical design, with filter-based fluorescence optics for high transmission and high sensitivity, and monochromator optics for convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization.

hybrid optics

   Variable bandwidth monochromators

Variable bandwidth monochromators optimize fluorophore detection

Cytation multimode configurations can include quad monochromators with variable bandwidths, selectable from 9 nm to 50 nm, in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.


   100 mW Alpha Laser


Laser-based excitation in Cytation 5 offers the power required to get great sensitivity and fast reading speeds, which are critical for Alpha assays.

 Label-free cell counting for cell proliferation studies

   Peltier Cooling for environmental stability

Label-free cell counting

The Peltier Cooling Module facilitates interior cool down after incubated processes, to allow efficient switching between multiple applications without unwanted temperature influences. For temperature sensitive assays typically run at ambient, or room temperature, the Cooling Module maintains environmental stability within Cytation, allowing < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation.


Fluorescence intensity
Fluorescence polarization
Time resolved fluorescence
Filtered luminescence
Alpha detection
UV-Vis absorbance
Fluorescence intensity
Time resolved fluorescence*
Variable bandwidth fluorescence monochromators
Upright microscope
Upgradable to add inverted microscope
Take3 Plate compatible
Incubation to 65 °C
Incubation to 45 °C

*TRF is a secondary mode in monochromator-only configurations

All configurations are compatible with optional dual reagent injector, CO2/O2 controller, BioStack and BioSpa 8.


Detection modes UV-Vis absorbance
Fluorescence intensity
Fluorescence polarization (Cytation 5/1)
Time-resolved fluorescence (Cytation 5/1)
Alpha (Cytation 5)
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C (Cytation 7/1) with Condensation Control™
4-Zone incubation to 65 °C (Cytation 5) with Condensation Control
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Models for both CO2 and O2 or CO2 only are available
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments (Cytation 7/5)
200 - 999 nm, 1 nm increments (Cytation 1)
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm) (Cytation 7/5)
2.4 nm (Cytation 1)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
<1% at 3.0 OD (Cytation 7)
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity
Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (top/bottom) (Cytation 7/5)
Filters / dichroic mirrors
Wavelength range Monochromators: 250 - 700 nm (900 nm option) (Cytation 7/5)
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth Variable; from 9 to 50 nm, in 1 nm increments (Cytation 7/5)
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator (Cytation 7/5):
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity Monos: 20 amol ATP (flash) (Cytation 7/5)
Filters: 10 amol ATP (flash), 100 amol (glow)
Fluorescence Polarization
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 280 - 700 nm (850 nm option)
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (secondary mode) (Cytation 5)
Filters (top)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Filters: Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate) (Cytation 5)
Alpha Detection
Light source 100 mW 680 nm laser (Cytation 5)
Detector PMT (Cytation 5)
Wavelength selection Filters (top) (Cytation 5)
Sensitivity 100 amol LCK peptide (384-well low volume plate) (Cytation 5)
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri dishes
Dead volume 1.1 mL, 100 µL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Physical Characteristics
Power 150 Watts to 250 Watts, configuration dependent
Dimensions 20.2" D x 16.4" W x 17.5" H
(51.4 cm D x 41.6 cm W x 44.5 cm H)
Weight 65 lbs to 80 lb (29 kg to 36.3 kg), configuration dependent
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.