Cytation 5 Cell Imaging Multi-Mode Reader

Imaging & Microscopy > Cell Imaging Multi-Mode Readers

Cytation 5 Cell Imaging Multi-Mode Reader

Overview:

Cytation™ 5 Cell Imaging Multi-Mode Reader combines automated digital widefield microscopy with conventional multi-mode microplate reading in a unique, patented design. With Cytation 5, you can capture rich phenotypic cellular information and well-based quantitative data in a single experiment. Cytation 5, along with Gen5 software, automates the workflow from image capture, processing, and analysis to create publication-ready images and data. Augmented Microscopy™ is the combination of all of these features in one compact system.
 

PROMO: Save $1,000 when you purchase BioTek’s new Fluorescence Test Plate. Valid worldwide through 9/29/17.


Features:

Combined digital microscopy and multi-mode detection

 
Combined digital microscopy and multi-mode detection
Cytation 5’s patented design incorporating automated digital microscopy with filter- and/or monochromator-based multi-mode detection is configurable – add modules as your research requirement change over time. Along with Gen5 software, Cytation captures both phenotypic and quantitative data within a single experiment.

 

Patented Hybrid optics: sensitivity of filters, flexibility of monochromator

 
Patented Technology
Cytation 5’s hybrid optical design offers filter-based fluorescence optics for high transmission and high sensitivity, while the available monochromator optics offers convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization.

 

Variable bandwidth monochromators optimize fluorophore detection

 
Variable bandwidth monochromators
The bandwidths of the monochromators in Cytation 5’s fluorescence detection module are selectable from 9 nm to 50 nm, in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.

 

100 mW Alpha Laser: Speed and performance-based excitation for Alpha assays

 
100 mW Alpha Laser
Laser-based excitation offers the power required to get great sensitivity and fast reading speeds, which are critical for Alpha assays.

 

CO2/O2 control, incubation and shaking for live cell assays

 
Live cell assays
Cytation 5 is well suited for live cell assays, with temperature control to 65 °C, plus linear and orbital shaking. A gas controller module is available to help regulate CO2 and O2 levels in the system for longer-term live cell requirements. A dual reagent injector provides quick inject/image capability for observing fast reactions.

Label-free cell counting for cell proliferation studies

Label-free cell counting
It’s fast and simple to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.

Models:

Imaging Plus Multi-Mode Configurations

Part #
CYT5FV
CYT5FAV
CYT5MV
CYT5MPV
CYT5MFV
CYT5MFAV
IMAGING            
Fluorescence imaging
Brightfield imaging
Color brightfield imaging
Phase contrast imaging      
   
FILTER-based            
Fluorescence intensity
 
 
Fluorescence polarization
 
 
Time resolved fluorescence
 
 
Filtered luminescence
 
 
Alpha detection
 
 
 
 
MONOCHROMATOR-based            
UV-Vis absorbance    
Fluorescence intensity    
Time resolved fluorescence (secondary)    
Luminescence    
OTHER            
Take3 Plate compatible    

Imaging Only and Multi-Mode Only Configurations

Part #
CYT5V
CYT5PV
CYT5MF CYT5MFA CYT5F CYT5FA CYT5M
IMAGING              
Fluorescence imaging
 
       
Brightfield imaging
 
       
Color brightfield imaging
 
       
Phase contrast imaging  
         
FILTER-based              
Fluorescence intensity
 
 
 
Fluorescence polarization
 
 
 
Time resolved fluorescence
 
 
 
Filtered luminescence
 
 
 
Alpha detection
 
 
 
 
 
MONOCHROMATOR-based              
UV-Vis absorbance    
   
Fluorescence intensity    
   
Time resolved fluorescence (secondary)    
   
Luminescence    
   
OTHER              
Take3 Plate compatible    
   

All configurations include incubation to 65 °C, shaking, and are compatible with BioTek’s Dual Reagent Injection Module, CO2/O2 Gas Controller and BioStack Microplate Stacker.


Specifications:

General
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 65 °C with Condensation Control; +0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2% at 1% O2
Models for both CO2 and O2 or CO2 only are available
Imaging System
Imaging mode Fluorescence
Brightfield
Color Brightfield
Phase Contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit gray scale, Sony CCD, 1.25 megapixel. 0.3 µm/pixel at 20x
Objective capacity 6 user-replaceable objectives
Objectives available 1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions User-trained autofocus, autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software control
Joystick control (option)
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutes

Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 3 colors, 4x, <7 minutes, 30 seconds
Image Analysis Software option Gen5 Image+: Advanced image analysis
Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features
Fluorescence Intensity
Light source Xenon flash
Detector PMT for monochromator system
PMT for filter system
Wavelength selection Quad monochromators (top/bottom)
Filters (top)
Wavelength range Monochromators: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Detection system Two PMT detectors: one for monochromator system, one for filter system
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Luminescence
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity Monos: 20 amol ATP (flash)
Filters: 10 amol ATP (flash), 100 amol (glow)
Fluorescence Polarization
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 280 - 700 nm (850 nm option)
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (secondary mode)
Filters (top)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Filters: Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate)
Absorbance
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Alpha Detection
Light source 100 mW 680 nm laser
Detector PMT
Wavelength selection Filters (top)
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Reagent Injectors (option)
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates, Petri dishes
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Physical Characteristics
Power 250 Watts max.
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 80 lbs (36.3 Kg)
Regulatory
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.