Cytation 5 Cell Imaging Multi-Mode Reader

Cell Imaging & Microscopy > Cell Imaging Multi-Mode Readers

Cytation 5 Cell Imaging Multi-Mode Reader

Overview:

 

Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast for maximum applications reach.  The multi-mode detection modules include filter- and monochromator-based fluorescence  detection, luminescence and UV-Vis absorbance detection. Gen5™ software provides complete control over all imaging and data capture, plus powerful image and data analysis.
 

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Features:

green box Multi-mode plate reader with sophisticated imaging

Multi-mode plate reader with sophisticated imaging

Cytation 5 extends the legacy of BioTek's multi-mode plate readers with a modular and upgradable imaging mode. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. Information on cell morphology, localization of signal, cell count and more is obtained with Cytation 5’s imaging mode.

Plate reading: absorbance, fluorescence; luminescence; advanced read modes

Imaging: fluorescence; phase contrast; HC brightfield; brightfield; color brightfield

green box Cytation 5: Ready for any assay

Cytation 5: Ready for any assay

With its combination of hybrid plate reader and advanced microscopy mode, Cytation 5 is truly ready for any assay. Contact us to learn how Cytation 5 can transform your lab and greatly increase your productivity.

green box Advanced microscopy: Unlimited possibilities

Advanced microscopy: Unlimited possibilities

Cytation 5 automates many traditionally manual microscopy tasks, from slide scanning to time-lapse live cell assays; from low to high magnification. Cytation 5 is ready for any imaging assay.

Flexible hardware: 6-objective turret, 1.25x to 60x, 20+ colors available, wide FOV camera
Full automation: automated stage, autofocus, automated turret
Live cell imaging: temperature and gas (CO2 and O2) control for time-lapse live cell imaging

green box Hit-picking: Multi-mode detection + imaging saves time and data storage

Hit-picking

(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.

green box Powerful image processing and analysis

Powerful image processing and analysis

No need to process and analyze images one by one on a dedicated computer. In Gen5, pre-program your analysis tasks and walk away.

Image processing: stitching, Z-projection, deconvolution, digital phase contrast
Image analysis: cell count, confluence, cytoplasm analysis, intracellular analysis, subpopulation analysis, signal translocation and much more.

 

green box  Hybrid plate reader: Flexibility and performance

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Cytation 5 is an advanced plate reader
that delivers both the flexibility and performance you need for any microplate assay in your lab.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser

green box  Variable bandwidth for sensitivity and specificity

Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation uses a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance.

green box Micro-volume analysis with Take3 plate

Micro-volume analysis with Take3 plate

Turn your Cytation 5 into a micro-volume analysis system with Take3. You can run 16 or 48 samples in one run to save a lot of time compared to single-sample devices. Gen5 is pre-programmed for ssDNA, dsDNA, RNA and protein quantification in 2 μL.


Models:

Imaging Plus Multi-Mode Configurations

Part #
CYT5FV
CYT5FW
CYT5FAV
CYTFAW
CYT5MV
CYT5MW
CYT5MPV
CYT5MPW
CYT5MFV
CYT5MFW CYT5MFAV CYT5MFAW
Fluorescence imaging
Brightfield imaging
Color brightfield imaging
Phase contrast imaging
           
       
Wide FOV camera
 
 
 
 
 
 
Filter-based fluorescence intensity
 
 
 
 
Filter-based fluorescence polarization
 
 
 
 
Filter-based time resolved fluorescence
 
 
 
 
Filter-based luminescence
 
     
Filter-based Alpha detection
 
 
 
 
 
 
 
 
Monochromator-based UV-Vis absorbance
       
Monochromator-based fluorescence intensity
       
Monochromator-based time resolved fluorescence (secondary)
       
Monochromator-based Luminescence
       
Take3 Plate compatible
       

Imaging Only and Multi-Mode Only Configurations

Part #
CYT5V
CYT5W
CYT5PV
CYT5PW CYT5MF CYT5MFA CYT5F CYT5FA CYT5M
Fluorescence imaging
 
       
Brightfield imaging
 
       
Color brightfield imaging
 
       
Phase contrast imaging
   
         
Wide FOV camera
 
 
         
Filter-based fluorescence intensity
 
 
 
 
 
Filter-based 
fluorescence polarization
 
 
 
 
 
Filter-based time resolved fluorescence
 
 
 
 
 
Filter-based luminescence
 
     
 
Filter-based Alpha detection
 
 
 
   
 
 
Monochromator-based UV-Vis absorbance
       
   
Monochromator-based Fluorescence intensity
       
   
Monochromator-based Time resolved fluorescence (secondary)
       
   
Monochromator-based Luminescence
       
   
Take3 Plate compatible
       
   

All configurations include incubation to 65 °C and shaking, and are compatible with BioTek’s Dual Reagent Injection Module, CO2/O2 Gas Controller, and BioStack Microplate Stacker, and BioSpa Automated Incubator.


Specifications:

General
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 65 °C with Condensation Control;
+0.2 °C at 37 °C
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides rapid cooling after incubated processes.
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Image+ and Image Prime software available for full image analysis
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Models for both CO2 and O2 or CO2 only are available
Imaging System
Imaging mode Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit Sony CMOS, standard or wide FOV
Objective capacity 6 user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, auto LED intensity, auto exposure
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software control
Joystick control (option)
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutes

Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 3 colors, 4x, <7 minutes, 30 seconds
Burst Mode: 10 fps, single well, single color at <= 5Oms integration time
Image Analysis Software option Gen5 Image+: Advanced image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features
Fluorescence Intensity
Light source Xenon flash
Detector PMT for monochromator system
PMT for filter system
Wavelength selection Quad monochromators (top/bottom)
Filters (top)
Wavelength range Monochromators: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Luminescence
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity Monos: 20 amol ATP (flash)
Filters: 10 amol ATP (flash), 100 amol (glow)
Fluorescence Polarization
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 280 - 700 nm (850 nm option)
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (secondary mode)
Filters (top)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Filters: Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate)
Absorbance
Light source Xenon flash
Detector photodiode
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Alpha Detection
Light source 100 mW 680 nm laser
Detector PMT
Wavelength selection Filters (top)
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Reagent Injectors (option)
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates, Petri dishes
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Physical Characteristics
Power 250 Watts maximum consumption.
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 80 lbs (36.3 kg)
Regulatory
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.