Synergy Neo2 Hybrid Multi-Mode Reader

Microplate Readers: Multi-Mode and Absorbance Readers > Multi-Mode Microplate Readers

Synergy Neo2 Hybrid Multi-Mode Reader


Synergy™ Neo2 Multi-Mode Microplate Reader is designed for the screening laboratory, with speed and ultra-high performance. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF and up to 4 PMTs provide ultra-fast measurements with excellent results. For walk-away automation and high throughput, BioStack Neo is the fastest on the market, and the barcode-labeled filter cubes help streamline workflows and limit errors. Synergy Neo2 features BioTek’s patented Hybrid Technology™, with its independent optical paths that ensure uncompromised performance in all detection modes. Advanced environmental controls, including CO2/O2 control, incubation to 65 °C and variable shaking support live cell assays; cell-based detection is optimized with direct bottom illumination. Powerful Gen5 Software offers complete reader control, powerful data analysis, and automation and LIMS integration.


Patented Hybrid Technology

Hybrid Tech
Hybrid Technology offers many benefits for screening and other multi-mode workflows, including the flexibility to easily select a discrete wavelength and perform spectral scans for excitation or emission peaks. Filter-based fluorescence provides fast wavelength switching and excellent sensitivity.


Multiple PMT detectors


Multiple PMTs
Up to 4 PMTs are available in the highly configurable, modular Synergy Neo2, for ultra-fast measurements in all detection modes. Two top PMTs meet the requirements for ultrafast ratiometric assays.


TRF laser and Alpha laser


TRF laser
The TRF laser provides ultra-fast measurement speed, along with optimal sensitivity for critical screening applications like TRF, and TR-FRET. Laser-based detection ensures the best performance for Alpha assays.


Variable bandwidth monochromators


Variable bandwith
Synergy Neo2 has quadruple scientific monochromators, with the fluorescence module offering variable bandwidths from 3 nm to 50 nm, selectable in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.


Live-cell assay optimized


Live cell assay optimized
Temperature control to 65 °C, plus linear and orbital shaking and an available gas controller module optimize the environment in Synergy Neo2 for live cell assays. The dedicted filter-based bottom optical path directly illuminates the plate bottom, increasing the sensitivity of live cell measurements, while a dual reagent injector provides quick inject/image capability for observing fast reactions.


Gen5 Data Analysis Software


Live cell assays
Gen5 Microplate Reader and Imager Software doesn’t compromise on usability or data analysis performance – protocol creation is intuitive and flexible, and the data analysis capability rivals standalone packages. For the GxP lab, Gen5 Secure software offers features to help comply with 21 CFR Part 11, including electronic signature, data security and multiple level permissions.


  Monochromator   Filter
  ABS        FI and LUM Dual filter top FI/FP/TRF and LUM Single filter top FI/FP/TRF and LUM Filter bottom FI/FP/TRF Alpha laser TRF laser


Detection modes UV-Vis absorbance
Fluorescence intensity
Fluorescence polarization
Time-resolved fluorescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types 6- to 1536-well plates
Other labware supported Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 65 °C with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: + 0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2%at 1% O2
Models for CO2 /O2 or CO2 only are available
Barcode reader 1D and 2D camera-based scanner
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Monochromator bandwidth <3 nm (230 - 285 nm), <6 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 well: 6 seconds
Fluorescence Intensity
Light source Xenon flash
Detector Dual top PMTs
Single top PMT (option)
Low noise PMT (bottom filter system)
Red shifted PMT (top/bottom monochromator system)
Wavelength selection Quad monochromators (top/bottom)
Filters (top/bottom)
Wavelength range Monochromators: 250 - 850 nm
Filters (dual PMT): 200 - 850 nm
Monochromator bandwidth Variable, from 3 to 50 nm in 1 nm increments
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.2 pM (4 amol/well, 20 µL, 384-well low volume plate) - top
Fluorescein 1 pM (10 amol/well, 10 µL, 1536-well plate) - top
Fluorescein 1 pM (0.1 fmol/well, 100 µL, 384-well plate) - bottom

Quad monochromator:
Fluorescein 2 pM (40 amol/well, 20 µL, 384-well low volume plate) - top
Fluorescein 2.5 pM (0.25 fmol/well, 100 µL, 384-well plate) - bottom
Reading speed (kinetic) 96 well: 6 seconds
384 well: 11 seconds
1536 well: 25 seconds
Wavelength range 300 - 700 nm
Dynamic range > 6 decades
Sensitivity 5 amol ATP typical (384-well low volume plate)
Fluorescence Polarization
Light source Xenon flash
Detector Dual PMT or single PMT (option)
Wavelength selection Filters
Wavelength range 280 - 850 nm
Sensitivity 1 mP standard deviation at 1 nM fluorescein
Time-Resolved Fluorescence
Light source Xenon flash or TRF laser (option)
Detector Dual PMT or single PMT (option)
Wavelength selection Filters (top/bottom)
Wavelength range Monos: 250 - 850 nm
Filters (dual PMT): 200 - 850 nm
Sensitivity With TRF laser: 5 fM (384-well low volume plate)
With Xenon flash lamp: 40 fM (384-well low volume plate)
Alpha Detection
Light source 100 mW 680 nm laser
Detector PMT
Wavelength selection Filters (top)
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Read speed 96 well: 30 seconds
384 well: 1 minute 50 seconds
1536 well: 7 minutes 20 seconds
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy + 1 µL or 2%
Dispense precision < 2% at 50-200 µL
Physical Characteristics
Power 250 Watts maximum consumption.
Dimensions Without TRF laser:
15.4" W x 20.7" D x 16.1" H
(39.2 x 52.5 x 40.8 cm)

With TRF laser:
15.4" W x 24.2" D x 16.1" H
(39.2 x 61.4 x 40.8 cm)
Weight Without TRF laser: 78 lbs. (35.4 kg)
With TRF laser: 100 lbs. (45.3 kg)
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.