405 LS Washer

Liquid Handling: Microplate Washers, Dispensers, and Pipetting Systems > Microplate Washers

405 LS Washer

Overview:

The 405™ LS Microplate Washer is the fifth generation BioTek washer, known for superior performance and reliability for washing protocols ranging from gentle cell washing to vigorous ELISAs. The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions. The optional BioStack™ is available for walk-away automation of up to 50 microplates.
 

Features:

Intuitive onboard User Interface or LHC™ pc software

 
LHC

The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions.

Magnetic and polystyrene bead assays, conventional ELISAs

 
Plates

The 405 LS is ideal for many standard plate washing and dispensing protocols, but it also automates more complex operations like magnetic and polystyrene bead washing workflows – and it’s Luminex® xMap® approved. Bead washing is available for 96- and 384-well microplates.

 

Patented Dual-Action™ manifold

 
405LS

Cell-based assays are easily processed with the 405 Select LS, with its angled dispense tubes, extensive aspirate and dispense rates and plate position controls providing optimal conditions for washing even fragile cell layers.


Models:

Part #
384-well
96-well
Optimized Cell Washing
4-Buffer Switching
Biomagnetic Separation**
Vacuum Filtration**
405LSHT
 *
   
 
405LSHTV
 *
 
 
405LSU
     
405LSUV
 •
 •
 
 
405LSUS
 
 
405LSUSQ
 •
 •
 
405LSUVS
 
405LSUVSQ
 •
 •
405LSR
 
 
 
   
405LSRV
 
 
   
405LSRS
 
 
 
 
405LSRSQ
 
   
405LSRVS
 
 
 
405LSRVSQ
 
 

* 96-well washing available with optional manifold PN 1170010, sold separately
** With available accessory module sold separately

 

405™ LS Microplate Washer Configurations
  Feature Design
HT Faster 384-well microplate washing
V Automated Internal 4-Buffer Switching  
S Ultrasonic Advantage™
  • Built-in ultrasonic cleaner for manifold self-maintenace
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action™
R 96-well microplate washing
  • 96-tube (8 x 12) manifold
Q Verify™ Technology
  • Built-in clog detection for dispense and aspirate tubes

Specifications:

General
Microplate types 96- and 384-well
Low profile and standard height
Solid and filter bottom (option)
Onboard software Create, edit or run multiple protocols
Software (computer control) LHC2 pc software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Separation Biomagnetic separation, vacuum filtration (optional)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
Ultrasonic Advantage™ Yes (option)
Verify™ clog detection Yes (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
Washing
Manifold types 96-well washing: 96-tube manifold - straight dispense tubes
96- and 384-well washing: 96-tube Dual-Action manifold - 20° angled dispense tubes
384-well washing (fast): 192-tube Dual-Action manifold - 7° angled dispense tubes
Volume range 25 to 3,000 µL/well, in 1 µL increments
Wash cycles 1 - 250
Buffer/reagent selection Auto switching (internal) for up to 4 buffers (option)
Supply bottle 4 L or 10 L (optional)
Dispense precision <3% CV: 300 µL/well (96-well washing)
<4% CV: 80 µL/well (384-well washing)
Residual volume <2 µL/well (96- & 384-well plates)
96-tube manifold for 96 wells; 192-tube for 384 wells
Wash speed 96-wells, 300 µL/well, 3 cycles; <30 seconds
384-wells,100 µL/well, 3 cycles: <80 seconds
384-wells, 400 µL/well, 1 cycle: <20 seconds
Flow rates High flow to low flow
Optimized rates for cell assays
Sterilization Chemical
Vacuum filtration Selectable: Approximate final at 30 seconds:
Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg

Vacuum filtration time range: 5 to 999 seconds
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions 14" W x 17" D x 10" H
(35.6 x 43.2 x 25.4 cm)
Weight With internal buffer switching - 36 lbs (16.5 kg)
Without - 30 lbs (13.5 kg)
Regulatory
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.