405 TS Washer

Liquid Handling: Microplate Washers and Dispensers > Microplate Washers

405 TS Washer


The BioTek 405 TS washer has an excellent reputation in life science research for outstanding results in many applications. The 405 TS has a unique set of features designed to efficiently wash cell-based assays, microsphere-based assays, and ELISA applications. Programming and operation via the touchscreen interface is intuitive and simple. The patented Verify technology automatically checks for manifold tube blockages before a wash cycle, and Ultrasonic Advantage automatically cleans any blocked manifold tubes. These functions save time and eliminate frustration while ensuring successful results.


blue The gold standard of microplate washers

The gold standard of microplate washers

blue Cell-friendly design for high quality results

Cell-friendly design for high quality results

Cell-friendly design for high quality resultsA combination of angled dispense tubes and both highly adjustable dispense and aspiration rate settings make 405 TS the ideal washer for cell-based assays. The gentle flow rates and side-wall dispensing position ensure the integrity of cell monolayers.

blue Automate magnetic and filtration bead-based assays

Automate magnetic and filtration bead-based assays

xmapMultiplexed bead-based assays are very common, and 405 TS easily accommodates necessary wash steps. Magnet accessories for biomagnetic separation workflows, available in both flat and 4-zone ring designs, ensure high bead recovery (1). A vacuum filtration module is available for polystyrene bead assays (2).

blue Self-maintaining design with patented ultrasonic bath

Self-maintaining design with patented ultrasonic bath

Manifold tube clogs are the most common source of failure on plate washers. The built-in Ultrasonic Advantage allows the instrument to clean buildup in the manifold tube automatically, without removing it from the instrument.

blue Hands-off self-testing Verify™ Technology Option

Hands-off self-testing Verify Technology

The Verify Technology option eliminates tedious manual procedures by using an ultrasonic probe (1) to check dispense and aspirate performance. Test results are automatically displayed (2) and indicate tubes that may require additional cleaning.


Part #
Optimized Cell Washing
4-Buffer Switching
Biomagnetic Separation**
Vacuum Filtration**

* 96-well washing available with optional manifold PN 1170010, sold separately
** With available accessory module sold separately


405 TS Microplate Washer Configurations
  Feature Design
HT Faster 384-well microplate washing
V Automated Internal 4-Buffer Switching  
S Ultrasonic Advantage
  • Built-in ultrasonic cleaner for manifold self-maintenance
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action
R 96-well microplate washing
  • 96-tube (8 x 12) manifold
Q Verify technology
  • Built-in clog detection for dispense and aspirate tubes


Microplate types 96- and 384-well
Low profile and standard height
Solid and filter bottom (option)
Onboard software Create, edit or run multiple protocols
Software (computer control) LHC2 pc software (option)
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Separation Biomagnetic separation, vacuum filtration (optional)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
Ultrasonic Advantage™ Yes (option)
Verify™ clog detection Yes (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator and BenchCel Microplate Handler compatible
Manifold types 96-well washing: 96-tube manifold - straight dispense tubes
96- and 384-well washing: 96-tube Dual-Action manifold - 20° angled dispense tubes
384-well washing (fast): 192-tube Dual-Action manifold - 7° angled dispense tubes
Volume range 25-3,000 µL/well (192-pin manifold) 50-3,000 µL/well (96-pin manifold)
Wash cycles 1 - 250
Buffer/reagent selection Auto switching (internal) for up to 4 buffers (option)
Supply bottle 4 L or 10 L (optional)
Dispense precision <3% CV: 300 µL/well (96-well washing)
<4% CV: 80 µL/well (384-well washing)
Residual volume < 2 µL/well (96- & 384-well plates)
96-tube manifold for 96 wells; 192-tube for 384 wells
Wash speed 96-wells, 300 µL/well, 3 cycles; <30 seconds
384-wells,100 µL/well, 3 cycles: <80 seconds
384-wells, 400 µL/well, 1 cycle: <20 seconds
Flow rates High flow to low flow
Optimized rates for cell assays
Sterilization Chemical
Vacuum filtration Selectable: Approximate final at 30 seconds:
Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg

Vacuum filtration time range: 5 to 999 seconds
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions 14" W x 17" D x 10" H
(35.6 x 43.2 x 25.4 cm)
Weight With internal buffer switching - 36 lbs (16.5 kg)
Without - 30 lbs (13.5 kg)
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.