Epoch 2 Microplate Spectrophotometer

Microplate Readers: Multi-Mode and Absorbance Readers > Absorbance Microplate Readers

Epoch 2 Microplate Spectrophotometer


The BioTek Epoch 2 microplate spectrophotometer delivers excellent performance for UV-Vis absorbance measurements. Measurements can be performed in 6- to 384-well microplates, cuvettes, and in microvolume samples with the available Take3 microvolume plate. The broad wavelength range enables applications from nucleic acid and protein quantification in the low UV to microbial growth assays higher wavelengths. Epoch 2 is easy to use and the optional touch screen interface makes it simple to choose predefined protocols or to define custom programs. Endpoint, kinetic, spectral scanning and well area scanning modes, plus incubation and shaking enable wide-ranging applications.


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Filter-free UV-Vis wavelength selection from 200 – 999 nm

Epoch 2
The monochromator-based optics in Epoch 2 allows selection of wavelengths in 1 nm increments – no need to purchase filters! The broad wavelength range enables applications from nucleic acid and protein quantification in the low UV to microbial growth assays, and more.


End point, kinetic and spectral scanning

The onboard software in Epoch 2 enables several read modes including single and dual wavelength end point and kinetic measurements. Spectral scanning provides a convenient, quick spectrum of all defined samples. Under Gen5 software control, well area scanning is also available, along with powerful data analysis.
Touch screen


Compatible with 6- to 384-well plates and cuvettes for assay versatility

   Full Gen5™ data analysis onboard or PC for all read modes
The optional cuvette port enables quick and easy 1 cm sample measurements, making Epoch 2 a versatile choice for assay workflows in a variety of labware.

4-Zone™ incubation to 65 °C and Condensation Control™

Some important applications like biofuels research and food & beverage safety and quality call for higher incubation temperatures than typically expected. Epoch 2 can meet these assay requirements. Kinetic assays are optimized with Condensation Control to prevent interference caused by condensation on plate lids.
Advanced incubator design

Micro-volume (2 µL) measurements with the Take3 Plate

100 mW Alpha Laser
The Take3 Micro-Volume plates expand Epoch 2’s capabilities to measure 2 µL samples for direct DNA, RNA and protein quantification. Get rapid results for up to 48 samples at a time without additional instrumentation.

Available touchscreen interface and onboard software

Access several common end point, kinetic or micro-volume assays through the convenient touchscreen. Displayed data can be output to a printer or USB flash drive for added flexibility. Epoch 2 comes with Gen5 software for additional powerful data analysis. 


Part #
Gen5 Software
Cuvette port

Epoch 2

Epoch 2 touch screen


Epoch 2 is available in both non-touch and touchscreen configurations.


Detection modes UV-Vis absorbance
Read methods Endpoint, kinetic, absorbance spectral scanning (onboard software and under Gen5 control)
Endpoint, kinetic, absorbance spectral scanning, well area scanning (under Gen5 control)
Microplate types 6- to 384-well plates
Other labware supported Take3 Micro-Volume Plates (onboard software)
Take3 and Take3 Trio Micro-Volume Plates (under Gen5 control)
Standard cuvette (option)
Temperature control 4-Zone™ incubation to 65 °C with Condensation Control
Shaking Linear, orbital, double orbital
Software Onboard software with easy programming and several predefined protocols (touchscreen configurations)
Full reader control and data analysis and reporting under Gen5 Software control (Gen5 Software included)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 - 999 nm, selectable in 1 nm increments
Monochromator bandwidth 2.9 nm
Dynamic range 0 - 4.0 OD
Resolution 0.001 OD (onboard software)
0.0001 OD (under Gen5 control)
Pathlength correction yes (under Gen5 Control)
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 2.5 OD
OD linearity <1% from 0 to 2.5 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells sweep read: 8 seconds
384 wells sweep read: 14 seconds
Physical Characteristics
Power External 24 VDC power supply compatible with 100-240 volts AC at 50-60Hz.
60 W maximum consumption.
Dimensions Touch screen models: 12.5” H x 12.75” W x 15.5” D (32 x 32.3 x 39.54 cm)
Non-touch screen models approximately 8.5” H x 12.75” W x 15.5” D (22 x 32.3 x 39.54 cm)
Weight 25 lbs (11.34 kg)
Regulatory CE and TUV marked, RoHS compliant. Models for In Vitro Diagnostic use are available.