MultiFlo FX Multi-Mode Dispenser

Liquid Handling > Multi-Mode Washer Dispensers

MultiFlo FX Multi-Mode Dispenser

Overview:

MultiFlo™ FX is an automated reagent dispenser for 6- to 1536-well microplates. Its unique Parallel Dispense™ technology allows up to four independent reagents to be dispensed in parallel without potential carryover. What makes MultiFlo FX “multi-mode” is its configurability: you can add a wash module or the RAD™ module, for random access individual well dispensing and bulk dispensing larger wells. Everything you need for fast, intuitive programming and operation is available through the color touchscreen. MultiFlo FX configured with either the wash module or RAD technology replaces up to five liquid handlers in a single compact platform.

 

Features:

Multi-mode dispensing can replace up to four dispensers and a washer

 
MultiFlo FX

Providing unsurpassed liquid handling flexibility, MultiFlo FX’s modular base can accommodate 1 or 2 peristaltic pumps, a 2-syringe pump module and a wash module or a single channel dispense. MultiFlo FX takes the place of multiple instruments, but is compact and very simple to use.

Parallel Dispense™ technology: peristaltic and/or syringe pumps

 
Parallel dispense

Peristaltic pumps and syringe pumps each offer benefits; peri-pumps help save precious reagent, while syringe pumps are fast and precise. MultiFlo FX can be configured with 2 peri and 2 syringe pumps, allowing up to four reagents to be dispensed in parallel, without carryover.


RAD™ technology for dispensing to individual wells

 
8 to 1

When the liquid handling workflow calls for individual well dispensing along with rapid bulk dispensing, MultiFlo FX can handle it. The unique RAD module provides single channel dispensing to discrete wells of a 48-, 96- or 384-well plate. For rapid single channel dispensing to 6- to 24-well plate, the 8-to-1 RAD cassette is perfect for the task.


Cost savings through low prime volume and single tube access

 
Cost saving

With programmable prime volumes, backflush capability and the unique single tube access with RAD, you’ll save on costly reagents for all your dispensing processes.
 

Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers

 
Cells

For sensitive cell-based assays, the dispense tips on the MultiFlo FX dispense manifolds and wash manifolds are angled, allowing gentle reagent dispense down the well sides. This helps keep cell layers intact, even through multiple-step operations.

Walkaway automation with BioStack 4 or BioSpa 8 for lidded or unlidded plates

MultiFlo FX and BioStack 4

Integration BioStack to MultiFlo FX allows automated processing of multiple plates at a time, without manual intervention. MultiFlo FX integrated to BioSpa 8 adds automated media exchange and reagent addition to automated live cell workflows.


Models:

  MFX MFXP* MFXW** MFXPW** MFXR*** MFXPR***
Unconfigured base instrument
         
1 peripump dispenser only  
       
Wash module only    
     
1 peripump dispenser and wash module      
   
RAD technology with one peripump          
RAD technology with two peripumps          

 

*P configurations include one plastic tip cassette
**W configurations include 96/384 wash manifold
***R configurations include one plastic tip multi-channel or RAD cassette.


 

Dispenser Modules (purchased separately)

7210010 - External Peristaltic Pump Dispenser Module
Adds 1 peristaltic pump dispenser to MultiFlo FX base instruments. For 6- to 1536-well microplates. Includes 1 plastic tip cassette.
Note: 7210010 is not required and is not compatible with MFXR or MFXPR

7210008 - External Dual Syringe Dispenser Module, Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.

7210009 - External Dual Syringe Dispenser Module, Non-Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.

 

Note: See accessories tab for all available accessories.


Specifications:

General
Microplate types Dispensing (peri pump and syringe)
96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable)
Washing
96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration)
Onboard software Create, edit or run multiple protocols
Software (computer control) LHC2 Software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
USB ports (2) For protocol storage/transfer and for optional external mouse or keyboard
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
Washing
Manifold types 96- and 384-well washing: 8-tube manifold
6-, 12-, 24-, 48-well washing: Custom manifolds available
Volume range 20-30,000 µL/well
Wash cycles 1-10
Supply bottle 2 L
Dispense accuracy +3%
Dispense precision 96-/384-well plates, 300 µL/well: <3% CV
6-well plates, 5560 µL/well: <5% CV 
Residual volume 96-well plate, 300 µL/well: < 2 µL/well
Wash speed 96-wells, 8-tube manifold, 3 cycles, >300 µL/well: <130 seconds
Wash volume 20-30,000 µL/well
Waste bottle 2L
Flow rates 140-422 µL/well/second
Sterilization Chemical
Dispensing - Peristaltic pump (Multi-Channel)
Manifold types 1 x 8 - sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing
Fluid delivery 1 or 2 peristaltic pumps
Dispense speed 96 wells, 5 µL cass, 10 µL/ well: 3 seconds
96 wells, 5 µL cass, 20 µL/ well: 3.5 seconds
384 wells, 5 µL cass, 5 µL/ well: 6.5 seconds
384 wells, 10 µL cass, 10 µL/ well: 8 seconds
384 wells, 1 µL cass, 1 µL/ well: 6 seconds
1536 wells, 1 µL cass, 1 µL/ well: 21 seconds
Volume range 500 nL - 3,000 µL/well, selectable in 1 µL increments
Flow rates User programmable rates from high to low
Dispense performance 1 µL cassette: recommended range: 1 - 50 µL
Accuracy: +5% at 1 µL
Precision: <5% CV at 1 µL
<10% CV at 500 nL
Minimum Prime Volume: 1.20 mL

5 µL cassette: recommended range: 5 - 2,500 µL
Accuracy: +2.0% at 5 µL
Precision: <2.5% CV at 5 µL
Minimum Prime Volume: 4.23 mL

10 µL cassette: recommended range: 10 - 3,000 µL
Dispense Accuracy: +2.0% at 10 µL
Dispense Precision: <2.0% CV at 10 µL
Minimum Prime Volume: 7.36 mL
Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
5 µL Cassette: 1,000 96-well microplates at 50 µL well
10 µL Cassette: 1,000 96-well microplates at 100 µL well
Sterilization Autoclave, chemical
Dispensing - Syringe pump
Manifold types 96- and 384-well dispensing:
One 16-tube (2 x 8) manifolds - 316 SS tubes
Two 16-tube (1 x 16) manifolds - 316 SS tubes

1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 SS or 316 SS tubes
6- to 48-well dispensing: custom autoclavable manifolds available
Fluid delivery Two positive displacement syringe drives
Dispense speed 20 µL /well, 96 wells, 1 x16 tubes:: 5 seconds
20 µL /well, 384 wells, 1 x16 tubes:: 14 seconds
3 µL /well, 1536 wells, 2 x 32 tubes:: 7 seconds
Volume range 3 - 3,000 µL/well selectable in 1 µL increments
Minimum Prime Volume: 12 mL
Flow rates User programmable rates from high to low
Dispense accuracy +1 µL at 5 µL
+1 µL at 20 µL
+1% at 100 µL
Dispense precision <5% CV at 5 µL
<2.5% CV at 20 µL
<1% CV at 100 µL
Supply bottle 1 L or 2 L
Sterilization Chemical, autoclavable option
Dispensing - Peristaltic pump (RAD Technology)
Labware types Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile
standard height and deep well formats; PCR trays and microtubes
8-to-1 tip: 6-, 12-, and 24-well plates
Manifold Types RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle
RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute
Volume range 500 nL - 30,000 µL
Minimum prime volume 1 µL cass, 18": 90 µL ; 1 µL cass, 30": 150 µL
5 µL cass, 18": 320 µL; 1 µL cass, 30": 530 µL
10 µL cass, 18": 555 µL; 10 µL cass, 30": 920 µL
Dispense speed (high flow) 1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells)
1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells)
5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells)
5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells)
10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells)
10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells)
Dispense performance 1 µL cass (med), 0.5 µL/well: Precision 10% CV
1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV
1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV
5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV
5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV
10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV
10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV
8-to 1 cass(high), 40 µL/well: Accuracy + 4%
8-to 1 cass (high), >10 µL/well: Precision 2.5%CV
8- to 1cass(high), >80 µL/well: Accuracy + 2%
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions Base instrument: 17.19"W x 11.75" D x 8" H (43.51 x 29.21 x 20.32 cm)
Weight Base instrument:
19.5 lbs (8.8 Kg)
Regulatory
Regulatory CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available.