MultiFlo FX Multi-Mode Dispenser

Liquid Handling: Microplate Washers, Dispensers, and Pipetting Systems > Multi-Mode Washer Dispensers

MultiFlo FX Multi-Mode Dispenser

Overview:

MultiFlo™ FX is an automated multi-mode reagent dispenser for 6- to 1536-well microplates. MultiFlo FX incorporates several unique technologies in its modular design, such as Parallel Dispense, RAD™ Random Access Dispense and the new, patent-pending AMX™ Automated Media Exchange modules to facilitate a variety of liquid handling applications from 2D and 3D cell culture to concentration normalization assays, ELISA, bead-based assays and more. A fully configured MultiFlo FX replaces up to five liquid handlers, saving space, time and instrumentation budgets. MultiFlo FX integrates to the BioSpa™ 8 Automated Incubator and a BioTek imager or multi-mode reader, for complete workflow automation for many cell imaging and biochemical applications.

 

Features:

Multi-mode dispensing replaces multiple instruments

 
MultiFlo FX

Providing unsurpassed liquid handling flexibility, MultiFlo FX’s modular base can accommodate 1 or 2 peristaltic pumps, a 2-syringe pump module and a wash module or a single channel dispense. MultiFlo FX takes the place of multiple instruments, but is compact and very simple to use.

AMX™ module automates gentle media exchange

 
Parallel dispense

The patent-pending AMX module uses peristaltic pumps to gently add and remove media from the wells, without disturbing 2D, 3D cell structures (spheroids, tumoroids). It’s also useful for washing suspension cells.

 

RAD™ module for mapped dispensing

 
8 to 1
Import custom .CSV file for RAD dispensing

 

The unique RAD technology enables custom-mapped dispensing to individual wells to facilitate many applications including concentration normalization assays.

 

Wash module for 6- to 384-well plates

 
Wash module

The versatile wash module for MultiFlo FX enables 6- to 384-well washing with custom manifolds. Dispensing and aspirate tube positioning, fluid flow rate and wash parameters are fully customizable, and the angled dispense tubes provide gentle washing for cell-based assays. The module uses integrated dispense and waste pumps to keep the system compact.

Parallel Dispense™ technology: peristaltic and/or syringe pumps

 
Parallel dispense

Peristaltic pumps and syringe pumps each offer benefits; peri-pumps help save precious reagent, while syringe pumps are fast and precise. MultiFlo FX can be configured with 2 peri and 2 syringe pumps, allowing up to four reagents to be dispensed in parallel, without carryover.

Cell friendly angled dispense and/or wash tubes

 
Cells

For sensitive cell-based assays, the dispense tips on the MultiFlo FX dispense manifolds and wash manifolds are angled, allowing gentle reagent dispense down the well sides. This helps keep cell layers intact, even through multiple-step operations.


Models:

Part # Description
MFXP1 MultiFlo FX with 1 module arm, 1 peristaltic dispense pump, 1 peri-pump cassette with plastic tips.
MFXP2 MultiFlo FX with 2 module arms, 1 peristaltic dispense pump. Includes 1 peri-pump cassette with plastic tips.
MFXP2R MultiFlo FX with RAD single channel dispenser. Includes 2 module arms, 2 peristaltic dispense pumps, 1 RAD manifold, and tubing included.

 

Optional Modules

 
  Compatible With MFXP1 MFXP2 MFXP2R

7210010

Secondary peripump module. Includes module and choice of (1) one peri-pump cassette with plastic tips.  
7210008 Dual syringe pump module (autoclavable). Includes module with two syringe pumps, (2) autoclavable bottles (PN 7140511) and (1) autoclavable tubing set (PN 7183006). Manifold(s) are required and must be purchased separately, see "Syringe Pump Dispenser Module" section for available manifolds.
7210009 Dual syringe pump module (non-autoclavable).  Includes module with two pumps, (2) non-autoclavable bottles (PN 4070515) and (1) non-autoclavable tubing set (PN 7183002).  Manifold(s) are required and must be purchased separately, see "Syringe Pump Dispenser Module" section for available manifolds.
1260023 Wash module. Includes pump, bottles, tubing and one 96/384 wash manifold. Other manifolds available, order separately.  
1260041 AMX Automated Media Exchange module. Includes secondary peri pump module, AMX dispense and aspirate manifolds. Two cassettes (PN 1260035) are required, order separately.    
MFXP2 - 7210009 - 1260023



Specifications:

General
Microplate types Dispensing (peri pump and syringe)
96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable)
Washing
96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration)
Onboard software Create, edit or run multiple protocols
Software (computer control) LHC2 Software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
USB ports (2) For protocol storage/transfer and for optional external mouse or keyboard
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
Washing
Manifold types 96- and 384-well washing: 8-tube manifold
6-, 12-, 24-, 48-well washing: Custom manifolds available
Volume range 20-30,000 µL/well
Wash cycles 1-10
Supply bottle 2 L
Dispense accuracy +3%
Dispense precision 96-/384-well plates, 300 µL/well: <3% CV
6-well plates, 5560 µL/well: <5% CV 
Residual volume 96-well plate, 300 µL/well: < 2 µL/well
Wash speed 96-wells, 8-tube manifold, 3 cycles, >300 µL/well: <130 seconds
Wash volume 20-30,000 µL/well
Waste bottle 2L
Flow rates 140-422 µL/well/second
Sterilization Chemical
Dispensing - Peristaltic pump (Multi-Channel)
Manifold types 1 x 8 - sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing
Fluid delivery 1 or 2 peristaltic pumps
Dispense speed 96 wells, 5 µL cass, 10 µL/ well: 3 seconds
96 wells, 5 µL cass, 20 µL/ well: 3.5 seconds
384 wells, 5 µL cass, 5 µL/ well: 6.5 seconds
384 wells, 10 µL cass, 10 µL/ well: 8 seconds
384 wells, 1 µL cass, 1 µL/ well: 6 seconds
1536 wells, 1 µL cass, 1 µL/ well: 21 seconds
Volume range 500 nL - 3,000 µL/well, selectable in 1 µL increments
Flow rates User programmable rates from high to low
Dispense performance 1 µL cassette: recommended range: 1 - 50 µL
Accuracy: +5% at 1 µL
Precision: <5% CV at 1 µL
<10% CV at 500 nL
Minimum Prime Volume: 1.20 mL

5 µL cassette: recommended range: 5 - 2,500 µL
Accuracy: +2.0% at 5 µL
Precision: <2.5% CV at 5 µL
Minimum Prime Volume: 4.23 mL

10 µL cassette: recommended range: 10 - 3,000 µL
Dispense Accuracy: +2.0% at 10 µL
Dispense Precision: <2.0% CV at 10 µL
Minimum Prime Volume: 7.36 mL
Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
5 µL Cassette: 1,000 96-well microplates at 50 µL well
10 µL Cassette: 1,000 96-well microplates at 100 µL well
Sterilization Autoclave, chemical
Dispensing - Syringe pump
Manifold types 96- and 384-well dispensing:
One 16-tube (2 x 8) manifolds - 316 SS tubes
Two 16-tube (1 x 16) manifolds - 316 SS tubes

1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 SS or 316 SS tubes
6- to 48-well dispensing: custom autoclavable manifolds available
Fluid delivery Two positive displacement syringe drives
Dispense speed 20 µL /well, 96 wells, 1 x16 tubes:: 5 seconds
20 µL /well, 384 wells, 1 x16 tubes:: 14 seconds
3 µL /well, 1536 wells, 2 x 32 tubes:: 7 seconds
Volume range 3 - 3,000 µL/well selectable in 1 µL increments
Minimum Prime Volume: 12 mL
Flow rates User programmable rates from high to low
Dispense accuracy +1 µL at 5 µL
+1 µL at 20 µL
+1% at 100 µL
Dispense precision <5% CV at 5 µL
<2.5% CV at 20 µL
<1% CV at 100 µL
Supply bottle 1 L or 2 L
Sterilization Chemical, autoclavable option
Dispensing: RAD (Random Access Module)
Labware types Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile
standard height and deep well formats; PCR trays and microtubes
8-to-1 tip: 6-, 12-, and 24-well plates
Manifold Types RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle
RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute
Volume range 500 nL - 30,000 µL
Minimum prime volume 1 µL cass, 18": 90 µL ; 1 µL cass, 30": 150 µL
5 µL cass, 18": 320 µL; 1 µL cass, 30": 530 µL
10 µL cass, 18": 555 µL; 10 µL cass, 30": 920 µL
Dispense speed (high flow) 1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells)
1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells)
5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells)
5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells)
10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells)
10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells)
Dispense performance 1 µL cass (med), 0.5 µL/well: Precision 10% CV
1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV
1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV
5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV
5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV
10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV
10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV
8-to 1 cass(high), 40 µL/well: Accuracy + 4%
8-to 1 cass (high), >10 µL/well: Precision 2.5%CV
8- to 1cass(high), >80 µL/well: Accuracy + 2%
Media Exchange: AMX (Automated Media Exchange Module)
Microplate types 96- and 384-well standard (ANSI/SBS)
Manifold types Two 8-channel autoclavable manifolds, tube-in-tube design
Cassettes Autoclavable cassettes with with 5 µL tubing
Dispense Precision <5% CV
Dispense Accuracy <5%
Aspiration uniformity <5%
Sterilization method Cassettes and manifolds: chemical and autoclavable
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions Base instrument: 17.19"W x 11.75" D x 8" H (43.51 x 29.21 x 20.32 cm)
Weight Base instrument:
19.5 lbs (8.8 Kg)
Regulatory
Regulatory CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available.