Synergy H1 Hybrid Multi-Mode Reader

Detection > Multi-Mode Microplate Readers

Synergy H1 Hybrid Multi-Mode Reader

Overview:

Synergy™ H1 is a configurable multi-mode microplate reader. You can choose monochromator-base optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patent Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s needs change. Top and bottom fluorescence intensity, UV-visible absorbance and luminescence detection are available to meet many life science research requirements.
 

 

 


Features:

Cost-effective hybrid offering excellent sensitivity, flexibility and value

 
Synergy H1
BioTek’s Hybrid Technology offers affordable multi-mode detection without compromising on performance. Sensitive filter-based optics, flexible monochromator-based optics, or both – on one instrument, powered by Gen5 software for data collection and analysis.

 

Modular and upgradable: choose the modes and modules you need now, add as needs change

Modular and upgradable
With six different base configurations to choose from, Synergy H1 is an affordable, upgradable solution to address your laboratory’s research needs now and well into the future.

 

Patented Hybrid Technology™: sensitivity of filters and flexibility of monochromators

Patented Technology
Synergy H1’s hybrid optical design offers filter-based fluorescence optics for high transmission and high sensitivity, while the available monochromator optics offers convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization

Low volume (2 µL) dsDNA quantification with Take3 Micro-Volume Plates

Tak3
The Take3 Micro-Volume plate extends the Synergy H1 capabilities beyond standard microplate reading, by measuring up to 48, 2 µL samples at one time for rapid nucleic acid and protein quantification. Absorbance and fluorescence measurements are quick and easy, no additional instrumentation is required!

Live cell assay friendly with temperature control and CO2/O2 control

Synergy H1
Synergy H1 offers temperature control to 45 °C, plus linear and orbital shaking. Expand the applications base to live cell assays by adding the CO2/02 controller or the dual reagent injector for quick inject/read processes.

Models:

Part #
H1M
H1MD
H1F
H1FD
H1MF
H1MFD
Monochromator fluorescence
 
 
Monochromator absorbance
 
 
Full-light luminescence
Filter/dichroic fluorescence  
 
Fluorescence polarization    
Time resolved fluorescence
• *
• *
Filtered luminescence    
Dual reagent dispenser  
 
 
Temperature control to 45°C
Gen5 data analysis software
* Secondary mode            
             
Gas Controller Compatible Configurations** H1MG H1MDG H1FG H1FDG H1FMG H1MFDG
** These configurations have the same features as the non- Gas Controller Compatible configurations in the chart above. Gas Controller sold separately.

 


Specifications:

General
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types 6- to 384-well plates
Other labware supported Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone™ incubation to 45 °C with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Data Analysis Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2% at 1% O2
Models for both CO2 and O2 or CO2 only are available
Absorbance
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Monochromator bandwidth 4 nm (230-285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy ±2 nm
Monochromator wavelength repeatability ±0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity
Light source Xenon flash
Detector PMT for monochromator system
PMT for filter system
Wavelength selection Quad monochromators (top/bottom)
Filters (top)
Wavelength range Monochromators: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth Fixed, 16 nm
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Luminescence
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity Monos: 20 amol ATP (flash)
Filters: 10 amol ATP (flash), 100 amol (glow)
Fluorescence Polarization
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 280 - 700 nm (850 nm option)
Sensitivity 1.2 mP standard deviation at 1 nm fluorescein
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (secondary mode)
Filters (top)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Filters: Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate)
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increment
Dispense accuracy ±1 µL or 2%
Dispense precision <2% at 50-200 µL
Physical Characteristics
Power 130 Watts max.
Dimensions 15.4"W 18.6"D 12.9"H
(39.1 x 47.2 x 32.8 cm)
Weight 50 lbs (22.5 kg)
Regulatory
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.