Synergy H1 Hybrid Multi-Mode Reader

Microplate Readers: Multi-Mode and Absorbance Readers > Multi-Mode Microplate Readers

Synergy H1 Hybrid Multi-Mode Reader


Synergy™ H1 is a configurable multi-mode microplate reader, with monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers applications versatility and excellent performance in a modular platform to expand as your laboratory’s needs change. Synergy H1 now offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection; the fluorescence bandwidth can be set between 9 nm and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.


green box Cost effective Hybrid™ multi-mode reader

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Synergy H1 is a versatile plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab. Synergy H1 is synonymous with performance and value.

green box Variable bandwidth monochromators

Variable bandwidth for sensitivity and specificity

Synergy H1 offers quad monochromator optics with variable bandwidth. The bandwidth can be set between 9 nm and 50 nm, in 1 nm increments. Large bandwidths (1) provide increased sensitivity and lower limits of detection. Small bandwidths (2) provide increased specificity when multiple signals are present, reducing crosstalk and enhancing assay performance.

green box Modular and upgradable

Advanced microscopy: Unlimited possibilities

With both monochromator- and filter-based modules, environmental controls and reagent injectors, Synergy H1 is an affordable, upgradable solution to address many critical applications today and well into the future.

green box Micro-volume quantification  Micro-volume analysis with Take3 plate
Enable micro-volume analysis with the Synergy H1, using the Take3 Plate. Measure up to 16 or 48 samples in one run and save a lot of time, compared to single-sample devices. Gen5 has customizable protocols for ssDNA, dsDNA, RNA and protein quantification in 2 µL.

green box Live cell assay friendly environment

Micro-volume analysis with Take3 plate
Temperature control to 45 °C, CO2/O2 control, condensation control and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays.


Part #
Monochromator fluorescence
Variable bandwidth monochromator fluorescence
Monochromator absorbance
Full-light luminescence
Filter/dichroic fluorescence
Fluorescence polarization
Time resolved fluorescence
• *
• *
Filtered luminescence
Temperature control to 45°C
Gen5 data analysis software
Gas Controller Compatible Configurations**

* Secondary mode
** These configurations have the same features as the non- Gas Controller Compatible configurations in the chart above. Gas Controller sold separately.
All configurations are compatible with the Dual Reagent Injector Module, sold separately.


Detection modes UV-Vis absorbance
Fluorescence intensity
Fluorescence polarization
Time-resolved fluorescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types 6- to 384-well plates
Other labware supported Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone™ incubation to 65 °C with Condensation Control™
+0.5 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2% at 1% O2
Models for both CO2 and O2 or CO2 only are available
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Monochromator bandwidth 4 nm (230-285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Pathlength correction yes
Monochromator wavelength accuracy ±2 nm
Monochromator wavelength repeatability ±0.2 nm
OD accuracy <1% at 2.0 OD
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity
Light source Xenon flash
Detector PMT for monochromator system
PMT for filter system
Wavelength selection Quad monochromators (top/bottom)
Filters (top)
Wavelength range Monochromators: 250 - 700 nm (900 nm option)
Filters: 200 - 700 nm (900 nm option)
Monochromator bandwidth Fixed, 16 nm
Variable; from 9 to 50 nm, in 1 nm increments ("H1M2" configurations)
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity Monos: 20 amol ATP (flash)
Filters: 10 amol ATP (flash), 100 amol (glow)
Fluorescence Polarization
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 200 - 700 nm (900 nm option)
Sensitivity 2 mP standard deviation at 1 nm fluorescein
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Filters (top)
Wavelength range Filters: 200 - 700 nm (900 nm option)
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increment
Dispense accuracy ±1 µL or 2%
Dispense precision <2% at 50-200 µL
Physical Characteristics
Power 130 Watts maximum consumption.
Dimensions 15.4"W 18.6"D 12.9"H
(39.1 x 47.2 x 32.8 cm)
Weight 50 lbs (22.5 kg)
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.