Synergy H1 Hybrid Multi-Mode Reader

Microplate Readers: Multi-Mode and Absorbance Readers > Multi-Mode Microplate Readers

Synergy H1 Hybrid Multi-Mode Reader


Synergy™ H1 is a configurable multi-mode microplate reader, with monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers applications versatility and excellent performance in a modular platform to expand as your laboratory’s needs change. Synergy H1 now offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection; the fluorescence bandwidth can be set between 9 nm and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.


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green box Hybrid plate reader: Flexibility and performance

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Synergy H1 is an advanced plate reader
that delivers both the flexibility and performance you need for any microplate assay in your lab.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence

green box Upgradable to meet future application needs

Variable bandwidth for sensitivity and specificity

Synergy H1’s modular design allows you to start with what you need now, and add detection modes, gas control and dual reagent injectors as your laboratory’s workflows evolve.

green box Variable bandwidth for sensitivity and specificity

Advanced microscopy: Unlimited possibilities

Synergy H1 offers quad monochromator optics with variable bandwidth. The bandwidth can be set between 9 nm and 50 nm, in 1 nm increments. Large bandwidths (1) provide increased sensitivity and lower limits of detection. Small bandwidths (2) provide increased specificity when multiple signals are present, reducing crosstalk and enhancing assay performance.

green box Automated z-focus: best performance with all plate types Micro-volume analysis with Take3 plate
(1) Without automated z-focus available, performance at low volumes is affected. (2) With automated z-focus, reading height is precisely adjusted for best performance in all plate types and all volumes.

green box Extended dynamic range

Micro-volume analysis with Take3 plate
Synergy H1 offers an extended dynamic range, which allows detection of signals across a 7 log measurement range. Other systems can measure only small portions of the dynamic range of Synergy H1 using preset gains – this can cause reduced sensitivity on the low end or saturated signals on the high end of the assay signal range.

green box Environmental controls for cell-based assays

Micro-volume analysis with Take3 plate
Temperature control to 45 °C, CO2/O2 control and condensation control and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays.

green box Dual syringe injectors with specialized tips

Micro-volume analysis with Take3 plate
1) The robust precise dual syringe design eliminates the need for regular tubing replacement required by some peristaltic injector designs. Synergy H1 offers two tip types: (2) the straight tips enable vigorous mixing for rapid inject/read assays, and (3) the angled tip option won’t disturb cell layers for applications such as calcium kinetics.

green box  Micro-volume analysis with Take3 plate 

Micro-volume analysis with Take3 plate
Enable micro-volume analysis with the Synergy H1, using the Take3 Plate. Measure up to 16 or 48
samples in one run and save a lot of time, compared to single-sample devices. Gen5 has customizable
protocols for ssDNA, dsDNA, RNA and protein quantification in 2 µL.



Part #
Monochromator fluorescence
Variable bandwidth monochromator fluorescence
Monochromator absorbance
Full-light luminescence
Filter/dichroic fluorescence
Fluorescence polarization
Time resolved fluorescence
• *
• *
Filtered luminescence
Temperature control to 45°C
Gen5 data analysis software
Gas Controller Compatible Configurations**

* Secondary mode
** These configurations have the same features as the non- Gas Controller Compatible configurations in the chart above. Gas Controller sold separately.
All configurations are compatible with the Dual Reagent Injector Module, sold separately.


Detection modes UV-Vis absorbance
Fluorescence intensity
Fluorescence polarization
Time-resolved fluorescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types 6- to 384-well plates
Other labware supported Take3 Micro-Volume Plates
Temperature control 4-Zone™ incubation to 45 °C with Condensation Control™
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2% at 1% O2
Models for both CO2 and O2 or CO2 only are available
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Monochromator bandwidth 4 nm (230-285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Pathlength correction yes
Monochromator wavelength accuracy ±2 nm
Monochromator wavelength repeatability ±0.2 nm
OD accuracy <1% at 3.0 OD
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity
Light source Xenon flash
Detector PMT for monochromator system
PMT for filter system
Wavelength selection Quad monochromators (top/bottom)
Filters (top)
Wavelength range Monochromators: 250 - 700 nm (900 nm option)
Monochromator bandwidth Fixed, 16 nm
Variable; from 9 to 50 nm, in 1 nm increments ("H1M2" configurations)
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Sensitivity Monos: 20 amol ATP
Filters: 10 amol ATP
Fluorescence Polarization
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 280 – 700 nm (850 nm option)
Sensitivity 2 mP standard deviation at 1 nm fluorescein
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Filters (top)
Sensitivity <40 fM
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well microplates
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increment
Dispense accuracy ±1 µL or 2%
Dispense precision <2% at 50-200 µL
Physical Characteristics
Power 130 Watts maximum consumption.
Dimensions 14.75"W 18.25"D 13"H
(37.5 x 46.4 x 33 cm)
Weight <55 lbs (24.95 kg)
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.