MultiFlo FX Multi-Mode Dispenser
Technical Details
General
| Microplate types |
Dispensing (peri pump and syringe) 96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable) Washing 96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration) |
| Onboard software | Create, edit or run multiple protocols |
| Software (computer control) |
LHC2 Software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option) |
| Shaking |
Programmable up to 60 minutes Slow, medium, fast or variable |
| Soaking | Programmable up to 60 minutes |
| USB ports (2) | For protocol storage/transfer and for optional external mouse or keyboard |
| Automation |
BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
Washing
| Manifold types |
96- and 384-well washing: 8-tube manifold 6-, 12-, 24-, 48-well washing: Custom manifolds available |
| Volume range | 20-30,000 µL/well |
| Wash cycles | 1-10 |
| Supply bottle | 2 L |
| Dispense accuracy | +3% |
| Dispense precision |
96-/384-well plates, 300 µL/well: <3% CV 6-well plates, 5560 µL/well: <5% CV |
| Residual volume | 96-well plate, 300 µL/well: < 2 µL/well |
| Wash speed | 96-wells, 8-tube manifold, 3 cycles, >300 µL/well: <130 seconds |
| Wash volume | 20-30,000 µL/well |
| Waste bottle | 2L |
| Flow rates | 140-422 µL/well/second |
| Sterilization | Chemical |
Dispensing - Peristaltic pump (Multi-Channel)
| Manifold types | 1 x 8 - sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing |
| Fluid delivery | 1 or 2 peristaltic pumps |
| Dispense speed |
96 wells, 5 µL cass, 10 µL/ well: 3 seconds 96 wells, 5 µL cass, 20 µL/ well: 3.5 seconds 384 wells, 5 µL cass, 5 µL/ well: 6.5 seconds 384 wells, 10 µL cass, 10 µL/ well: 8 seconds 384 wells, 1 µL cass, 1 µL/ well: 6 seconds 1536 wells, 1 µL cass, 1 µL/ well: 21 seconds |
| Volume range | 500 nL - 3,000 µL/well, selectable in 1 µL increments |
| Flow rates | User programmable rates from high to low |
| Dispense performance |
1 µL cassette: recommended range: 1 - 50 µL Accuracy: +5% at 1 µL Precision: <5% CV at 1 µL <10% CV at 500 nL Minimum Prime Volume: 1.20 mL 5 µL cassette: recommended range: 5 - 2,500 µL Accuracy: +2.0% at 5 µL Precision: <2.5% CV at 5 µL Minimum Prime Volume: 4.23 mL 10 µL cassette: recommended range: 10 - 3,000 µL Dispense Accuracy: +2.0% at 10 µL Dispense Precision: <2.0% CV at 10 µL Minimum Prime Volume: 7.36 mL |
| Recommended cassette replacement interval |
1 µL Cassette: 1,000 384-well microplates at 5 µL well 5 µL Cassette: 1,000 96-well microplates at 50 µL well 10 µL Cassette: 1,000 96-well microplates at 100 µL well |
| Sterilization | Autoclave, chemical |
Dispensing - Syringe pump
| Manifold types |
96- and 384-well dispensing: One 16-tube (2 x 8) manifolds - 316 SS tubes Two 16-tube (1 x 16) manifolds - 316 SS tubes 1536-well dispensing: Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 SS or 316 SS tubes 6- to 48-well dispensing: custom autoclavable manifolds available |
| Fluid delivery | Two positive displacement syringe drives |
| Dispense speed |
20 µL /well, 96 wells, 1 x16 tubes:: 5 seconds 20 µL /well, 384 wells, 1 x16 tubes:: 14 seconds 3 µL /well, 1536 wells, 2 x 32 tubes:: 7 seconds |
| Volume range |
3 - 3,000 µL/well selectable in 1 µL increments Minimum Prime Volume: 12 mL |
| Flow rates | User programmable rates from high to low |
| Dispense accuracy |
+1 µL at 5 µL +1 µL at 20 µL +1% at 100 µL |
| Dispense precision |
<5% CV at 5 µL <2.5% CV at 20 µL <1% CV at 100 µL |
| Supply bottle | 1 L or 2 L |
| Sterilization | Chemical, autoclavable option |
Dispensing: RAD (Random Access Module)
| Labware types |
Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile standard height and deep well formats; PCR trays and microtubes 8-to-1 tip: 6-, 12-, and 24-well plates |
| Manifold Types |
RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute |
| Volume range | 500 nL - 30,000 µL |
| Minimum prime volume |
1 µL cass, 18": 90 µL ; 1 µL cass, 30": 150 µL 5 µL cass, 18": 320 µL; 1 µL cass, 30": 530 µL 10 µL cass, 18": 555 µL; 10 µL cass, 30": 920 µL |
| Dispense speed (high flow) |
1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells) 1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells) 5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells) 5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells) 10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells) 10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells) |
| Dispense performance |
1 µL cass (med), 0.5 µL/well: Precision 10% CV 1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV 1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV 5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV 5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV 10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV 10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV 8-to 1 cass(high), 40 µL/well: Accuracy + 4% 8-to 1 cass (high), >10 µL/well: Precision 2.5%CV 8- to 1cass(high), >80 µL/well: Accuracy + 2% |
Media Exchange: Automated Media Exchange Module
| Microplate types | 96- and 384-well standard (ANSI/SBS) |
| Manifold types | Two 8-channel autoclavable manifolds, tube-in-tube design |
| Cassettes | Autoclavable cassettes with with 5 µL tubing |
| Dispense Precision | <5% CV |
| Dispense Accuracy | <5% |
| Aspiration uniformity | <5% |
| Sterilization method | Cassettes and manifolds: chemical and autoclavable |
Physical Characteristics
| Power | 100 - 240 Volts AC. 50/60 Hz |
| Dimensions | Base instrument: 17.19"W x 11.75" D x 8" H (43.51 x 29.21 x 20.32 cm) |
| Weight |
Base instrument: 19.5 lbs (8.8 Kg) |
Regulatory
| Regulatory | CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available. |