product design

MultiFlo FX Multi-Mode Dispenser

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MultiFlo™ FX is an automated multi-mode reagent dispenser for 6- to 1536-well microplates. MultiFlo FX incorporates several unique technologies in its modular design, such as Parallel Dispense, Random Access Dispense and the new, patent-pending Automated Gentle Media Exchange modules to facilitate a variety of liquid handling applications from 2D and 3D cell culture to concentration normalization assays, ELISA, bead-based assays and more. A fully configured MultiFlo FX replaces up to five liquid handlers, saving space, time and instrumentation budgets. MultiFlo FX integrates to the BioSpa™ 8 Automated Incubator and a BioTek imager or multi-mode reader, for complete workflow automation for many cell imaging and biochemical applications.


What's New

Now with Automated, Gentle Media Exchange (AMX) and Custom-mapped RAD Dispensing!
RAD video
Automated Gentle Media Exchange of Suspension Cells using the AMX™ module for the MultiFlo FX
AMX video
MultiFlo FX - AMX for Gentle
Automated Media Exchange
RAD video
MultiFlo FX - RAD Mapped Dispensing


This compact, versatile dispenser enables a wide variety of applications for benchtop and integrated automation workflows.

Plate map for drug addition
Import custom RAD plate maps for concentration normalization assays

The configurable MultiFlo FX
The configurable MultiFlo FX: 4 reagent dispensers, washer, media exchange and single channel dispenser

These are just a few examples of the many important applications for MultiFlo FX. Visit our Applications page to see more.


BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!

Automate Your Workflows

Expand your workflow automation by integrating the MultiFlo FX with BioTek’s range of robotics, including the BenchCel Microplate Handler, BioStack™ Microplate Stacker and BioSpa™ Automated Incubator.


Multi-mode dispensing replaces multiple instruments

MultiFlo FX

Providing unsurpassed liquid handling flexibility, MultiFlo FX’s modular base can accommodate 1 or 2 peristaltic pumps, a 2-syringe pump module and a wash module or a single channel dispense. MultiFlo FX takes the place of multiple instruments, but is compact and very simple to use.

Automated, gentle media exchange for spheroids


The patent-pending Automated Media Exchange (AMX) module uses peristaltic pumps to gently add and remove media from the wells, without disturbing 2D, 3D cell structures (spheroids, tumoroids). It’s also useful for washing spheroids and suspension cells.

Parallel dispense

Mapped dispensing to individual wells

8 to 1
Import custom .CSV file for RAD dispensing

The unique RAD technology enables custom-mapped dispensing to individual wells to facilitate many applications including concentration normalization assays.


Wash module for 6- to 384-well plates


The versatile wash module for MultiFlo FX enables 6- to 384-well washing with custom manifolds. Dispensing and aspirate tube positioning, fluid flow rate and wash parameters are fully customizable, and the angled dispense tubes provide gentle washing for cell-based assays. The module uses integrated dispense and waste pumps to keep the system compact.

Wash module

Parallel Dispense™ technology: peristaltic and/or syringe pumps

Parallel dispense

Peristaltic pumps and syringe pumps each offer benefits; peri-pumps help save precious reagent, while syringe pumps are fast and precise. MultiFlo FX can be configured with 2 peri and 2 syringe pumps, allowing up to four reagents to be dispensed in parallel, without carryover.

Cell friendly angled dispense and/or wash tubes


For sensitive cell-based assays, the dispense tips on the MultiFlo FX dispense manifolds and wash manifolds are angled, allowing gentle reagent dispense down the well sides. This helps keep cell layers intact, even through multiple-step operations.


Product Reviews

Customer Spotlight

Mr. Charles TetaDepartment of Environmental Science and Health at the National University of Science and Technology (NUST)


In the Department of Environmental Science and Health at the National University of Science and Technology (NUST) in Bulawayo, Zimbabwe, Mr. Charles Teta specializes in ecotoxicology. His research includes uncovering the frequency and effects of endocrine disrupting chemicals in native fish, and analyzing hazardous chemicals such as heavy metals and pesticides in the environment that may have originated from municipal landfills, mines and urban effluents. With several kinetic and end-point assay techniques already employed, the manual processes were time consuming, and human error was an ever-present risk when dispensing into a microplate with a single-channel or multi-channel pipette. If reagents were added too slowly, or mixed incompletely, the assays had to be repeated, thereby doubling efforts, sample volume and costly materials.




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