product design

Cytation 5 Cell Imaging Multi-Mode Reader

Specifications

General

Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 65 °C with Condensation Control;
+0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Image+ and Image Prime software available for full image analysis
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Models for both CO2 and O2 or CO2 only are available

Imaging System

Imaging mode Fluorescence
Brightfield
Color Brightfield
Phase Contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit gray scale, Sony CCD
Objective capacity 6 user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, auto LED intensity, auto exposure
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software control
Joystick control (option)
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutes

Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 3 colors, 4x, <7 minutes, 30 seconds
Burst Mode: 10 fps, single well, single color at <= 5Oms integration time
Image Analysis Software option Gen5 Image+: Advanced image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features

Fluorescence Intensity

Light source Xenon flash
Detector PMT for monochromator system
PMT for filter system
Wavelength selection Quad monochromators (top/bottom)
Filters (top)
Wavelength range Monochromators: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds

Luminescence

Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity Monos: 20 amol ATP (flash)
Filters: 10 amol ATP (flash), 100 amol (glow)

Fluorescence Polarization

Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 280 - 700 nm (850 nm option)
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein

Time-Resolved Fluorescence

Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (secondary mode)
Filters (top)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Filters: Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate)

Absorbance

Light source Xenon flash
Detector photodiode
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds

Alpha Detection

Light source 100 mW 680 nm laser
Detector PMT
Wavelength selection Filters (top)
Sensitivity 100 amol LCK peptide (384-well low volume plate)

Reagent Injectors (option)

Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates, Petri dishes
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL

Physical Characteristics

Power 250 Watts max.
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 80 lbs (36.3 Kg)

Regulatory

Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

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