Cytation 5 Cell Imaging Multi-Mode Reader
Related Products
Compatible Products
BenchCel Microplate Handler
The BenchCel Microplate Handler is integrated to a variety of BioTek’s microplate washers, dispensers, readers and imagers to automate application-specific workflows. The system is modular and scalable to suit a range of applications including automated ELISA, add/read protocols and cell fixing, staining and imaging processes.
BioSpa 8 Automated Incubator
BioSpa 8 is an automated incubator linking BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.
BioStack Microplate Stacker
BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids. BioStack models equipped with a rotational gripper allow ergonomically friendly instrument panel access and compact integration positioning
Software
Gen5 Microplate Reader and Imager Software controls Cytation 5 for automated image capture, processing, analysis and development of publication-ready images and data. This imaging and microscopy automation represents BioTek’s Augmented Microscopy™ - the simplified, automated process to accomplish multiple microscopy steps in a single platform. Gen5 is available in several editions to meet the image analysis requirements across a range of applications.
Gen5 Image+ offers complete imaging control for Cytation 5, and provides image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.
Gen5 Image Prime controls Cytation 5 includes more expansive image processing and analysis: Z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.
Gen5 Secure editions add features to help ensure compliance to 21 CFR Part 11 requirements.
Gen5 add-on modules provide increased functionality for specific applications or analyses, such as Spot Counting.
Object area analysis in Gen5 software.
BioTek has made it easier than ever to run common applications with only a few clicks from Read to Results! The Quantitative Assay Apps use the power of Gen5 behind a simple, step-by-step setup customization of pre-defined protocols for a streamlined process. Automated data export and report tools complete the workflow requirements.
Peripherals
Application Starter Kits
Sample Files
AlphaScreen / AlphaLISA / SureFire
Fluorescence Polarization
Imaging
- 24-Well Corning FluoroBlok Kinetic Cell Migration-Invasion Sample Protocol (09-Oct-14)
- Hemocytometer Phase Contrast-Color Brightfield Cell Counting Sample Protocol (04-Nov-14)
- Cytation 5 Residual Protein A (14-Nov-14)
- 96-Well Trevigen CometAssayCometChip Imaging and Analysis (03-Dec-15)
- 24-Well ibidi HT-1080 Cytochalasin D Experiment File (29-Jun-16)
- 2D Scratch Wound Healing Assay Protocol File (29-Jun-16)
Sample Experiments with Data
System Files - Imaging Cubes
- Brightfield (31-May-13)
- CFP, PN 1225107 (31-May-13)
- CY5, PN 1225105 (31-May-13)
- CY7, PN 1225106 (31-May-13)
- DAPI, PN 1225100 (31-May-13)
- GFP, PN 1225101 (31-May-13)
- RFP, PN 1225103 (31-May-13)
- Texas Red, PN 1225102 (31-May-13)
- YFP, PN 1225104 (31-May-13)
- Acridine Orange, PN 1225109 (12-Feb-14)
- CFP-YFP-FRET (13-Feb-14)
- Propidium Iodide (PI), PN 1225111 (16-Jun-14)
- Chlorophyll, PN 1225112 (19-Aug-14)
- Phycoerythrin (19-Aug-14)
- TagBFP, PN 1225115 (14-Nov-14)
- GFP excitation - CY5 emission, PN 1225116 (25-Mar-15)
- RFP excitation - CY5 emission, PN 1225117 (25-Mar-15)
- Alexa 568 (09-Feb-16)
- EX 377 – EM 647, PN 1225121 (09-Feb-16)
- CFP FRET V2, PN 1225118 (19-Jul-16)
- CFP/YFP FRET V2, PN 1225119 (19-Jul-16)
- Oxidized roGFP2 Filter Cube, PN 1225122 (09-Aug-17)
- Ex 400 EM 647, PN 1225123 (17-Oct-17)
- TRITC, PN 1225125 (25-Sep-20)
System Files - Imaging Objectives
- 20x objective, Plan Fluorite WD 6.6 NA 0.45, PN 1220517 (08-Sep-13)
- 10x objective, Plan Fluorite WD 10 NA 0.3, PN 1220518 (08-Sep-13)
- 4x objective, Plan Fluorite WD 17 NA 0.13, PN 1220519 (09-Sep-13)
- 2.5x objective (2.75x eff.) Zeiss, Fluorite WD 6.3 NA: 0.12, PN 1220539 (09-Sep-13)
- 2.5x objective (2.25x eff.) Meiji, Plan Achromat, WD 6.2 NA: 0.07, PN 1220549 (09-Sep-13)
- 40x objective, Plan Fluorite WD 2.7 NA 0.6, PN 1220544 (18-Dec-14)
- 60x objective, Plan Fluorite WD 1.8 NA 0.7, PN 1220545 (18-Dec-14)
- 1.25x objective, Plan Apochromat WD 5 NA 0.04, PN 1220562 (13-Jan-15)
- 4x phase objective, Plan Fluorite WD 17 NA 0.13, PN 1320515 (13-Oct-16)
- 10x phase objective, Plan Fluorite WD 10 NA 0.3, PN 1320516 (13-Oct-16)
- 20x phase objective, Plan Fluorite WD 6.7 NA 0.45, PN 1320517 (13-Oct-16)
- 40x phase objective, Plan Fluorite WD 2.7 NA 0.6, PN 1320518 (13-Oct-16)
- 60x oil objective, Plan Apochromat WD 0.15 NA 1.42, PN 1450538 (13-Oct-16)
- 100x oil objective, Plan Apochromat WD 0.13 NA 1.4, PN 1450539 (13-Oct-16)
- 4x objective, Plan Achromat WD 18.5 NA 0.1, PN 1320546 (22-Nov-16)
System Files - Plate Types
- BioTek Counting Chamber (11-Jun-15)
- BioTek Adapter for Trevigen 96-well Comet Chip (01-Dec-15)
- iNCYTO C-chip (29-Oct-15)
- Ibidi µ-Slide VI (07-Jan-16)
- Ibidi µ-Slide | 0.6 Luer (07-Jan-16)
- Ibidi µ-Slide y-shaped (07-Jan-16)
- InSphero GravityTRAP assay plate (07-Jan-16)
- 1450541 Multi-vessel Adapter (25-Feb-16)
- Costar 3506 6-well plate (07-Mar-16)
- Costar 3512 12-well plate (07-Mar-16)
- Costar 3524 24-well plate (07-Mar-16)
- Costar 3548 48-well plate (07-Mar-16)
- MilliporeStageInsert (27-Jun-16)
- ONIXB04A (12-May-16)
- ONIXC04A (12-May-16)
- ONIXM04G (12-May-16)
- ONIXM04L (12-May-16)
- ONIXM04S (12-May-16)
- ONIXY04C (12-May-16)
- ONIXY04D (12-May-16)
- Greiner SensoPlate Plus, 384-well (13-May-16)
- T75 flask stage insert (27-Jul-16)
- T25 Flask Adapter for Corning PN 353108 (02-Mar-17)
- 4-position slide holder (28-Aug-17)
Accessories
Adapters and Labware Holders
- 02692Adapter for 75 x 50 mm slides and the Trevigen CometSlide HT
- 02747Petri dish adapter 35.5 mm, 4-position
- 02748Petri dish adapter 38.7 mm, 4-position
- 02749Petri dish adapter 60 mm, 2-position
- 1220548Slide adapter
- 1222210T25 cell culture flask adapter (1222210)
- 1222240Petri dish adapter 38.7 mm max
- 1222242Petri dish adapter 100 mm
- 1222246Petri dish adapter 35.5 mm max
- 1320016Counting Chamber Adapter
- 1322144Adapter, Trevigen CometSlide
- 1450541Multi-vessel adapter
- 16505164-position slide adapter
- 1652028Adapter for Falcon T25 cell culture flask
- 6002076Holder for PCR Microplates
BioSpa 8 Integration Kits
BioStack Integration Kits
Bottles & Tubing
- 1213032CO2 Flow meter and tubing
- 1213033N2 Flow meter and tubing
Filter Accessories
Filter Cubes
- 1325000Alpha filter block for 96 well measurements
- 1325001Alpha filter cube for 384 well measurements
- 8040501Blue filter set: EX 360/40 | EM 460/40 | DM 400 / Green filter set: EX 485/20 | EM 528/20 | DM 510
- 8040502Green filter set: EX 485/20 | EM 528/20 | DM 510 / Red filter set: EX 530/25 | EM 590/35 | DM 570
- 8040505HTRF filter set: EX 330/80 | EM 620/10, 665/8 | DM 365
- 8040506Filter Cube: LanthaScreen terbium filter set: EX 340/30 | EM 495/10, 520/25 | DM 400
- 8040507Filter Cube: Lance filter set: EX 360/40 | EM 620/10, 665/8 | DM 400
- 8040553Filter Cube – Luminescence
- 8040555TRF cube
- 8040561Green FP filter set: EX 485/20 | EM 528/20 | DM 510, polarizers
- 8040562Red FP filter set: EX 530/25 | EM 590/35 | DM 570, polarizers
- 8040563Blue/UV FP filter set: 360/40 EX, 460/40 EM, 400 nm mirror, UV polarizers
- 8040564Empty Filter Cube - Fluorescence Polarization Visible (excitation > 400nm)
- 8040565Empty Filter Cube - Fluorescence Polarization UV (excitation between 300 and 400 nm)
- 8040566Empty Filter Cube - non FP applications
- 8040574BRET 1: EM 460/40, 540/25
- 8040575BRET2: EM 410/80, 515/30
- 8040576Filter Block Asby 440 Blue/Green
- 8040578Deep UV FP 284/340
- 8040581Filter Block Asby Far Red FP
- 8040582Filter Block Asby Far RED
- 8040583Green/LUM Filter Cube - Green Filter Set: EX 485/20 | EM 528/10 | DM 510
- 8040584Filter Cube: Blue (360/460) / Red (530/590)
- 8040594Combination filter cube for MitoXpress and pH-Xtra Glycolysis assays
Filters
- 7082205400/30 nm Fluorescence Filter
- 7082206420/50 nm Fluorescence Filter
- 7082207440/40 nm Fluorescence Filter
- 7082208450/50 nm Fluorescence Filter
- 7082209485/40 nm Fluorescence Filter
- 7082210545/40 nm Fluorescence Filter
- 7082211560/20 nm Fluorescence Filter
- 7082212560/40 nm Fluorescence Filter
- 7082213620/40 nm Fluorescence Filter
- 7082214635/32 nm Fluorescence Filter
- 7082215560/15 nm Fluorescence Filter
- 7082218508/20 nm Fluorescence Filter
- 7082220360/40 nm Fluorescence Filter
- 7082221485/20 nm Fluorescence Filter
- 7082222460/40 nm Fluorescence Filter
- 7082223530/25 nm Fluorescence Filter
- 7082224590/35 nm Fluorescence Filter
- 7082225590/20 nm Fluorescence Filter
- 7082226645/40 nm Fluorescence Filter
- 7082227440/30 nm Fluorescence Filter
- 7082228380/20 nm Fluorescence Filter
- 7082229680/30 nm Fluorescence Filter
- 7082230340/30 nm Fluorescence Filter
- 7082242400/10 nm Fluorescence Filter
- 7082244580/50 nm Fluorescence Filter
- 7082245575/15 nm Fluorescence Filter
- 7082246516/20 nm Fluorescence Filter
- 7082247528/20 nm Fluorescence Filter
- 7082248310/20 nm Fluorescence Filter
- 7082249540/25 nm Fluorescence Filter
- 7082250320/20 nm Fluorescence Filter
- 7082251620/15 nm Fluorescence Filter
- 7082252600/40 nm Fluorescence Filter
- 7082253540/35 nm Fluorescence Filter
- 7082254340/11 nm Fluorescence Filter
- 7082256500/27 nm Fluorescence Filter
- 7082259284/10 nm Fluorescence Filter
- 7082263330/80 nm Fluorescence Filter
- 7082265620/10 nm Fluorescence Filter
- 7082266665/8 nm Fluorescence Filter
- 7082268495/10 nm Fluorescence Filter
- 7082270420/27 nm Fluorescence Filter
- 7082271565/10 nm Fluorescence Filter
- 7092146276/10 nm Fluorescence Filter
- 7092148280/10 nm Fluorescence Filter
- 7092150330/20 nm Fluorescence Filter
- 7092151355/20 nm Fluorescence Filter
- 7092152380/11 nm Fluorescence Filter
- 7092153395/25 nm Fluorescence Filter
- 7092154405/20 nm Fluorescence Filter
- 7092155410/10 nm Fluorescence Filter
- 7092157470/40 nm Fluorescence Filter
- 7092158475/20 nm Fluorescence Filter
- 7092160490/10 nm Fluorescence Filter
- 7092162500/20 nm Fluorescence Filter
- 7092163520/10 nm Fluorescence Filter
- 7092164520/20 nm Fluorescence Filter
- 7092166530/30 nm Fluorescence Filter
- 7092167532/40 nm Fluorescence Filter
- 7092168540/15 nm Fluorescence Filter
- 7092169540/45 nm Fluorescence Filter
- 7092170550/10 nm Fluorescence Filter
- 7092171556/10 nm Fluorescence Filter
- 7092172560/10 nm Fluorescence Filter
- 7092173570/10 nm Fluorescence Filter
- 7092174575/10 nm Fluorescence Filter
- 7092175580/10 nm Fluorescence Filter
- 7092176585/10 nm Fluorescence Filter
- 7092177590/10 nm Fluorescence Filter
- 7092178596/15 nm Fluorescence Filter
- 7092179610/10 nm Fluorescence Filter
- 7092180625/10 nm Fluorescence Filter
- 7092182645/10 nm Fluorescence Filter
- 7092183660/10 nm Fluorescence Filter
- 7092184665/34 nm Fluorescence Filter
- 7092185670/10 nm Fluorescence Filter
- 7092186670/20 nm Fluorescence Filter
- 7092187680/10 nm Fluorescence Filter
- 7092188680/20 nm Fluorescence Filter
- 7092189690/10 nm Fluorescence Filter
- 7092190700/20 nm Fluorescence Filter
- 7092191710/20 nm Fluorescence Filter
- 7092192730/40 nm Fluorescence Filter
- 7092193760/20 nm Fluorescence Filter
- 7092194760/34 nm Fluorescence Filter
- 7092195781/20 nm Fluorescence Filter
- 7092196785/20 nm Fluorescence Filter
- 7092197807/25 nm Fluorescence Filter
- 7092198818/40 nm Fluorescence Filter
- 7092200410/80 nm Fluorescence Filter
- 7092201515/30 nm Fluorescence Filter
- 7092205325/50 nm Fluorescence Filter
- 7092207615/10NM Lanthascreen Europium donor emission
- 7092208665/10NM Lanthascreen Europium acceptor emission
- 7092209LP610NM Chromaglo red emitting luciferase
- 7092210LP640NM Pierce Renilla-Firefly Dual Luciferase Red Firefly
- 7092211525/40NM Pierce Renilla-Firefly Dual Luciferase Green Renilla
Imaging Filter Cubes
- 1225100DAPI Filter cube
- 1225101GFP Filter cube
- 1225102Texas Red Filter cube
- 1225103RFP Filter cube
- 1225104YFP Filter cube
- 1225105CY5 Filter cube
- 1225106CY7 Filter cube
- 1225107CFP Filter cube
- 1225109Acridine Orange (+RNA) filter cube
- 1225111Propidium Iodide filter cube
- 1225112Chlorophyll A filter cube
- 1225113469/593 filter cube
- 1225114Cy5.5 filter cube
- 1225115TagBFP filter cube
- 1225116GFP excitation - CY5 emission
- 1225117RFP excitation - CY5 emission
- 1225118CFP FRET V2 filter cube
- 1225119CFP/YFP FRET V2 filter cube
- 1225121Ex 377 Em 647 filter cube
- 1225122Oxidized roGFP2 Filter Cube
- 1225123Ex 400 EM 647 filter cube
- 1225125TRITC imaging filter cube, EX 556 / EM 600
Imaging LED Cubes
- 1225001465 nm LED cube
- 1225002590 nm LED cube
- 1225003523 nm LED cube
- 1225004505 nm LED cube
- 1225005623 nm LED cube
- 1225006740 nm LED cube
- 1225007365 nm LED cube
- 1225008655nm LED cube
- 1225009390nm LED cube
- 1225011405 nm LED cube
- 1225012554 nm LED cube
Imaging Objectives
- 122051720x objective, Plan Fluorite WD 6.6 NA 0.45
- 122051810x objective, Plan Fluorite WD 10 NA 0.3
- 12205194x objective, Plan Fluorite WD 17 NA 0.13
- 12205392.5x objective (2.75x eff.) Zeiss, Fluorite WD 6.3 N.A: 0.12
- 122054440x objective, Plan Fluorite WD 2.7 NA 0.6
- 122054560x objective, Plan Fluorite WD 1.8 NA 0.7
- 12205492.5x objective (2.25x eff.) Meiji, Plan Achromat, WD 5.7 NA: 0.07
- 12205621.25x objective, Plan Apochromat WD 5 NA 0.04
- 13205154x phase objective, Plan Fluorite WD 17 NA 0.13
- 132051610x phase objective, Plan Fluorite WD 10 NA 0.3
- 132051720x phase objective, Plan Fluorite WD 6.7 NA 0.45
- 132051840x phase objective, Plan Fluorite WD 2.7 NA 0.6
- 1320520SSpare Phase Contrast Annulus for 4x Objective
- 1320521SSpare Phase Contrast Annulus for 10x / 20x Objective
- 1320523SSpare Phase Contrast Annulus for Brightfield
- 13205464x Objective, Olympus Plan Achromat WD 18.5 NA 0.1
- 1322026Spare Phase Contrast Annulus for 40x Objective (1322026)
- 1322500Spare Alignment Tool for 10x / 20x Phase Contrast Objectives
- 1322507Spare Alignment Tool for 40x Phase Contrast Objectives
- 145055520x objective Olympus Achromat
Mirrors
- 7132121Half-size dichroic mirror, 50%, Ex 200-850 nm, Em 200-850 nm
- 7137400Full-Size Dichroic Mirror, Ex. 320-390, Em. 410-800
- 7137435Full-Size Dichroic Mirror, Ex. 385-425, Em. 445-610
- 7137455Full-Size Dichroic Mirror, Ex. 400-450, Em. 460-710
- 7137510Full-Size Dichroic Mirror, Ex. 440-505, Em. 515-640
- 7137525Full-Size Dichroic Mirror, Ex. 475-520, Em. 530-670
- 7137545Full-Size Dichroic Mirror, Ex. 512-535, Em. 555-578
- 7137550Full-Size Dichroic Mirror, Ex. 415-540, Em. 560-850
- 7137570Full-Size Dichroic Mirror, Ex. 515-565, Em. 575-735
- 7137595Full-Size Dichroic Mirror, Ex. 540-590, Em. 600-770
- 7138320Half-Size Dichroic Mirror, Ex. 260-305, Em. 335-750
- 7138365Half-Size Dichroic Mirror, Ex. 290-350, Em. 380-800
- 7138400Half-Size Dichroic Mirror, Ex. 320-390, Em. 410-800
- 7138435Half-Size Dichroic Mirror, Ex. 385-425, Em. 445-610
- 7138455Half-Size Dichroic Mirror, Ex. 400-450, Em. 460-710
- 7138510Half-Size Dichroic Mirror, Ex. 440-505, Em. 515-640
- 7138525Half-Size Dichroic Mirror, Ex. 475-520, Em. 530-670
- 7138545Half-Size Dichroic Mirror, Ex. 512-535, Em. 555-578
- 7138550Half-Size Dichroic Mirror, Ex. 415-540, Em. 560-850
- 7138570Half-Size Dichroic Mirror, Ex. 515-565, Em. 575-735
- 7138595Half-Size Dichroic Mirror, Ex. 540-590, Em. 600-770
- 7138660Half-Size Dichroic Mirror, Ex. 580-655, Em. 665-850
- 8030515SEmpty Mirror Holder
- 8030516SFP Compatible Empty Mirror Holder
Miscellaneous
- 01503Calibrated CO2 Gas Tank
- 01985Spare Cover Slip for Counting Chamber
- 02682Corning® Costar® 24-well Clear TC-Treated Plates
- 02683Corning® 96-well Clear TC-Treated Plates
- 1210011Calibration Kit for CO2 Controller
- 1210012CO2 - only gas controller
- 1210013CO2 and O2 gas controller
- 1210514CO2 Regulator, inlet fitting meets CGA 320 standard.
- 1210515N2 Regulator, inlet fitting meets CGA 580 standard.
- 1220024Laser autofocus retrofit kit for field installed Cytation and Lionheart FX
- 1220521Anti-vibration plate
- 122253140x/60x Setup Plate
- 1225010Patented laser autofocus cube for Lionheart and all Cytation imaging configurations
- 1320003Joystick
- 1320023High Contrast Brightfield Kit for Label-Free Cell Counting
- 1322131Counting Chamber
- 1700508Anti-vibration table
- 1750006Set of 8 polypropylene scratch tips
- 7132166Optics Accessory Case
- TAKE3Take3 Micro-Volume Plate
- TAKE3TRIOTake3 Trio Micro-Volume Plate
Modules
- 1700001Peltier Cooling Module
- 8040036Dual Reagent Injector Module
Operators Manual
Product Qualification Packages
Reagent Injector Accessories
Software Options
- GEN5Gen5 Data Analysis Software
- GEN5AUTOROIAuto ROI module for Gen5 Image+ and Gen5 Image Prime
- GEN5IPLUSGen5 Image+
- GEN5IVDGen5 IVD
- GEN5IVDIPLUSGen5 IVD Image+
- GEN5SECUREGen5 Secure
- GEN5SECUREIPLUSGen5 Secure Image+
- GEN5SPOTGen5 Spot Counting Module
- Gen5IPRIMEGen5 Image Prime
- Gen5SECUREIPRIMEGen5 Secure Image Prime
Test Plates & Reagents
- 1400501Fluorescence Test Plate
- 7260522Absorbance Test Plate
- 7260551340 nm Absorbance Test Plate
- 8030015Luminescence Test Plate
Other available accessories for cell count & viability workflows include:
All-inclusive kit
