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product design

Cytation 5 Cell Imaging Multi-Mode Reader


green box Multi-mode plate reader with sophisticated imaging

Multi-mode plate reader with sophisticated imaging

Cytation 5 extends the legacy of BioTek's multi-mode plate readers with a modular and upgradable imaging mode. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. Information on cell morphology, localization of signal, cell count and more is obtained with Cytation 5’s imaging mode.

Plate reading: absorbance, fluorescence; luminescence; advanced read modes

Imaging: fluorescence; phase contrast; HC brightfield; brightfield; color brightfield

green box Cytation 5: Ready for any assay

Cytation 5: Ready for any assay

With its combination of hybrid plate reader and advanced microscopy mode, Cytation 5 is truly ready for any assay. Contact us to learn how Cytation 5 can transform your lab and greatly increase your productivity.

green box Advanced microscopy: Unlimited possibilities

Advanced microscopy: Unlimited possibilities

Cytation 5 automates many traditionally manual microscopy tasks, from slide scanning to time-lapse live cell assays; from low to high magnification. Cytation 5 is ready for any imaging assay.

Flexible hardware: 6-objective turret, 1.25x to 60x, 20+ colors available, wide FOV camera
Full automation: automated stage, autofocus, automated turret
Live cell imaging: temperature and gas (CO2 and O2) control for time-lapse live cell imaging

green box Hit-picking: Multi-mode detection + imaging saves time and data storage


(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.

green box Powerful image processing and analysis

Powerful image processing and analysis

No need to process and analyze images one by one on a dedicated computer. In Gen5, pre-program your analysis tasks and walk away.

Image processing: stitching, Z-projection, deconvolution, digital phase contrast
Image analysis: cell count, confluence, cytoplasm analysis, intracellular analysis, subpopulation analysis, signal translocation and much more.


green box  Hybrid plate reader: Flexibility and performance

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Cytation 5 is an advanced plate reader
that delivers both the flexibility and performance you need for any microplate assay in your lab.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser

green box  Variable bandwidth for sensitivity and specificity

Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation uses a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance.

green box Micro-volume analysis with Take3 plate

Micro-volume analysis with Take3 plate

Turn your Cytation 5 into a micro-volume analysis system with Take3. You can run 16 or 48 samples in one run to save a lot of time compared to single-sample devices. Gen5 is pre-programmed for ssDNA, dsDNA, RNA and protein quantification in 2 μL.