Cytation 5 Cell Imaging Multi-Mode Reader

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Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast for maximum applications reach. The multi-mode detection modules include filter- and monochromator-based fluorescence detection, luminescence and UV-Vis absorbance detection. Gen5™ software provides complete control over all imaging and data capture, plus powerful image and data analysis.

Testimonials

What's New

Purchase a Lionheart or Cytation and receive patented Laser Autofocus or Gen5 Image+ software Free!

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Scratch The Scratch Assay Starter Kit automates cell migration and wound healing assays, used along with BioTek’s Lionheart FX Automated Microscope or Cytation 5 Cell Imaging Multi-Mode Reader.

applications

With the ability to collect phenotypic and quantitative data in a single experiment, Cytation 5 is a powerful tool for imaging applications, and conventional multi-mode applications.

The Scratch Assay Starter Kit automates cell migration and wound healing assays:

AutoScratch automatically makes consistent, repeatable scratch wounds.

The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).

AutoScratch creates scratch wounds with excellent performance, compared to manual methods.

Rabbit spinal cord with H&E dyes, stitched montage

SH Sy5y cells at 40x

These are just a few examples of the many important applications for Cytation 5. Visit our Applications page to see more.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!

Seahorse XF Imaging and Normalization System

Cytation 5 is a key component of Agilent Technologies’ Seahorse XF Imaging and Normalization System. XFe Analyzers simultaneously measure the two major cellular energy-producing pathways, mitochondrial respiration and glycolysis, in live cells and in real time. The integrated solution with Cytation 5 combines cellular analysis and imaging for an intuitive workflow to streamline XF data interpretation.

Features

Multi-mode plate reader with sophisticated imaging

Cytation 5 extends the legacy of BioTek's multi-mode plate readers with a modular and upgradable imaging mode. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. Information on cell morphology, localization of signal, cell count and more is obtained with Cytation 5’s imaging mode.

Plate reading: absorbance, fluorescence; luminescence; advanced read modes

Imaging: fluorescence; phase contrast; HC brightfield; brightfield; color brightfield

Cytation 5: Ready for any assay

With its combination of hybrid plate reader and advanced microscopy mode, Cytation 5 is truly ready for any assay. Contact us to learn how Cytation 5 can transform your lab and greatly increase your productivity.

Advanced microscopy: Unlimited possibilities

Cytation 5 automates many traditionally manual microscopy tasks, from slide scanning to time-lapse live cell assays; from low to high magnification. Cytation 5 is ready for any imaging assay.

Flexible hardware: 6-objective turret, 1.25x to 60x, 20+ colors available, wide FOV camera
Full automation: automated stage, autofocus, automated turret
Live cell imaging: temperature and gas (CO₂ and O₂) control for time-lapse live cell imaging

Hit-picking: Multi-mode detection + imaging saves time and data storage

(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.

Powerful image processing and analysis

No need to process and analyze images one by one on a dedicated computer. In Gen5, pre-program your analysis tasks and walk away.

Image processing: stitching, Z-projection, deconvolution, digital phase contrast
Image analysis: cell count, confluence, cytoplasm analysis, intracellular analysis, subpopulation analysis, signal translocation and much more.

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Cytation 5 is an advanced plate reader
that delivers both the flexibility and performance you need for any microplate assay in your lab.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser

Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation uses a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance.

Micro-volume analysis with Take3 plate

Turn your Cytation 5 into a micro-volume analysis system with Take3. You can run 16 or 48 samples in one run to save a lot of time compared to single-sample devices. Gen5 is pre-programmed for ssDNA, dsDNA, RNA and protein quantification in 2 μL.

Product Reviews

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Customer Spotlight

Institut de Pharmacologie Moléculaire et Cellulaire

Novel, Highly Sensitive, Non-Radioactive Cytotoxicity Assay with Cytation 5

28-December-18

At the Institut de Pharmacologie Moléculaire et Cellulaire (IPMC) in Nice/Sophia-Antipolis, France, Drs. Fabienne Anjuère and Véronique Braud are on the cutting edge of immunotherapy research with the goals of identifying therapeutic targets and developing innovative vaccines and immunotherapies to treat inflammatory disorders and cancers. Most recently, they are focused on the study of innate immune cells (natural killer cells, neutrophils, dendritic cells) in healthy and tumorigenic epithelial tissues, which requires use of flow cytometry, cellular and biochemical assays in both in vitro and in vivo studies.

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