Cytation 1 Cell Imaging Multimode Reader
Technical Details
General
| Detection modes |
UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types |
Monochromator: 6- to 384-well plates Filters: 6- to 1536-well plates Imaging: 6- to 1536-well plates |
| Other labware supported |
Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Take3 Micro-Volume Plates |
| Temperature control | 4-Zone incubation to 45 °C with Condensation Control™; Variation +0.2 °C at 37 °C |
| Cooling | Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. |
| Shaking | Linear, orbital, double orbital |
| Software |
Gen5™ Microplate Reader and Imager Software included Gen5 Image+ and Image Prime software available for full image analysis Gen5 Secure available for 21 CFR Part 11 |
| Automation |
BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) |
0 - 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller Models for both CO2 and O2 or CO2 only are available |
Imaging - Widefield Microscope
| Imaging mode | Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x) |
| Imaging method | Single color, multi-color, montage, time lapse, z-stacking |
| Image processing | Z-projection, image stitching |
| Camera | Sony CCD, 16-bit gray scale |
| Objective capacity | 2-position automated turret for user-replaceable objectives |
| Objectives available | 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 |
| Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm |
| Automated functions | Autofocus, auto LED intensity, auto exposure |
| Autofocus method |
Image-based autofocus User-trained autofocus Laser autofocus (option) |
| Positional controls | Software control |
| Image collection rate |
Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 3 colors, 4x, 12 minutes Laser autofocus: 96 wells, 1 color (DAPI), 4x, <3 minutes 96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 50ms integration time |
| Image Analysis Software option |
Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure: 21 CFR Part 11 compliant features |
Fluorescence Intensity
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Deep blocking band pass filters / dichroic mirrors |
| Wavelength range | Filters: 200 - 700 nm (850 nm option) |
| Dynamic range | 7 decades |
| Sensitivity | Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate) |
| Reading speed (kinetic) |
96 wells: 11 seconds 384 wells: 22 seconds |
Luminescence
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity |
10 amol ATP (flash) 100 amol (glow) |
Fluorescence Polarization
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 – 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nM fluorescein |
Time-Resolved Fluorescence
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Sensitivity | Europium 40 fM (4 amol/well, 384-well plate) |
Absorbance
| Light source | Xenon flash |
| Detector | Photodiode |
| Wavelength selection | Monochromator |
| Wavelength range | 200 – 999 nm, 1 nm increment |
| Monochromator bandwidth | 2.4 nm |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | + 2 nm |
| Monochromator wavelength repeatability | + 0.2 nm |
| OD accuracy |
<1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) |
96 wells: 11 seconds 384 wells: 22 seconds |
Reagent Injectors (option)
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri and cell culture dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 - 1000 µL in 1 µL increments |
| Plate geometry | 6- to 384-well microplates |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 – 200 µL |
Physical Characteristics
| Power | 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min) |
| Dimensions |
16.4" W x 17.5" H x 20.2" D (41.6 cm x 44.5 cm x 51.4 cm) |
| Weight | 65 lbs (29 Kg) |
Regulatory
| Regulatory | CE and TUV marked. Models for In Vitro Diagnostic use are available. |