Cytation 1 Cell Imaging Multi-Mode Reader
Cytation™ 1 Cell Imaging Multi-Mode Reader combines fluorescence and high contrast brightfield imaging with conventional multi-mode detection without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. Cytation 1's multi-mode detection module includes high sensitivity filter-based fluorescence and a monochromator system for UV-Vis absorbance. Temperature control and shaking are standard; CO2/O2 control and reagent injectors are available. BioTek’s powerful Gen5 software makes image capture and plate reading easy.
- Affordable, patented, quantitative digital microscopy and optional multi-mode microplate detection
- Augmented Microscopy™ using Gen5 software for seamless image capture to quantitative publication ready data with no extensive training required
- Fluorescence and high contrast brightfield imaging from 1.25x to 60x to capture large regions of interest or intracellular details
- Affordable automation: automated XY stage, focus, exposure, image capture and LED intensity
- More than 20 imaging filter/LED cube colors reach many applications
- Cell friendly design: 4-Zone incubation to 45°C with Condensation Control, CO2/O2 control, angled injectors and lidded plate handling
- High performance filter-based fluorescence detection with convenience of monochromator based UV-Vis absorbance
- Peltier Cooling Module maintains environmental stability for uncompromised assay integrity
Seahorse XF Imaging and Normalization SystemCytation 1 is a key component of Agilent Technologies’ Seahorse XF Imaging and Normalization System. XFe Analyzers simultaneously measure the two major cellular energy-producing pathways, mitochondrial respiration and glycolysis, in live cells and in real time. The integrated solution with Cytation 1 combines cellular analysis and imaging for an intuitive workflow to streamline XF data interpretation.
Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly. Click here to request your free sample.
The Multi-Faceted Assays Enabled with Cytation TechnologyWATCH
Download our Application Note to discover how combining MitoXpress and pH-Xtra reagents with BioTek plate readers provide sensitive detection methods, including advanced lifetime (µs) time-resolved fluorescence (TRF), for getting the most meaningful data out of your cell metabolism assays.
The Scratch Assay Starter Kit automates cell migration and wound healing assays
The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).
These are just a few examples of the many important applications for Cytation 1. Visit our Applications page to see more.
BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!
BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.
Fluorescence and high contrast brightfield imaging
The powerful imaging module in Cytation 1 offers four color channels plus brightfield, from 1.25x to 60x to capture large regions of interest or intracellular details. More than 15 filter/LED cubes are available, supporting a broad range of imaging applications.
Cytation 1 has an automated XY stage, autofocus, auto exposure and auto LED intensity for efficient capture of high quality images. The automated features in Cytation 1 and Gen5 make it easy for you to find the region of interest, then quickly find the best settings to capture the images. Gen5 software is simple to use, without extensive training.
More than 20 filter/LED color cubes
BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.
Cell friendly design
Live cell assays require an optimal environment. Cytation 1 has temperature control and shaking, plus CO2 / O2 control, to facilitate longer-term kinetic studies.
Peltier Cooling for environmental stability
The Peltier Cooling Module promotes a rapid interior cool down after incubated processes, to allow efficient switching between multiple applications without unwanted temperature influences. For temperature sensitive assays typically run at ambient, or room temperature, the Cooling Module maintains environmental stability within Cytation, allowing < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation.
Customer SpotlightTime to Accelerate the Pace of Neurobiology Research
Differentiating a stem cell into a neuron or glial cell is typically a time-consuming bottleneck in human neurological dysfunction research. On top of this, precisely controlling the purity of sub-types in a mixed neuron population can be a complex task, yet is critical to improve assay reproducibility and eliminate unwanted cell types that may negatively impact results.