product design

Cytation 1 Cell Imaging Multi-Mode Reader

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Cytation™ 1 Cell Imaging Multi-Mode Reader combines fluorescence and high contrast brightfield imaging with conventional multi-mode detection without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. Cytation 1's multi-mode detection module includes high sensitivity filter-based fluorescence and a monochromator system for UV-Vis absorbance. Temperature control and shaking are standard; CO2/O2 control and reagent injectors are available. BioTek’s powerful Gen5 software makes image capture and plate reading easy.

   

Testimonials

 

Seahorse XF Imaging and Normalization System

Cytation 1 is a key component of Agilent Technologies’ Seahorse XF Imaging and Normalization System. XFe Analyzers simultaneously measure the two major cellular energy-producing pathways, mitochondrial respiration and glycolysis, in live cells and in real time. The integrated solution with Cytation 1 combines cellular analysis and imaging for an intuitive workflow to streamline XF data interpretation.

What's New

Filter CubePurchase a Synergy or Cytation Reader for use with Agilent MitoXpress or pH-Xtra Assays and receive a free filter cube!

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Download our Application Note to discover how combining MitoXpress and pH-Xtra reagents with BioTek plate readers provide sensitive detection methods, including advanced lifetime (µs) time-resolved fluorescence (TRF), for getting the most meaningful data out of your cell metabolism assays.


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Purchase a Lionheart or Cytation and receive patented Laser Autofocus or Gen5 Image+ software Free!
 

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applications

With the ability to collect phenotypic and quantitative data in a single experiment, Cytation 1 is a powerful tool for imaging applications, and conventional multi-mode applications.

 

The Scratch Assay Starter Kit automates cell migration and wound healing assays


AutoScratch automatically makes consistent, repeatable scratch wounds

AutoScratch automatically makes consistent, repeatable scratch wounds.


The Scratch Assay App automatically calculates % confluence,  wound width and maximum healing rate (max V)

The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).


The Scratch Assay Starter kit enables automated scratch wound assays with excellent performance, compared to manual methods.

AutoScratch creates scratch wounds with excellent performance, compared to manual methods.


Rabbit spinal cord
HeLa cells treated with SmartFlare™ CY5-GAPDH Reagent at 20x

High contrast brightfield for label-free cell counting.
High contrast brightfield for label-free cell counting.


These are just a few examples of the many important applications for Cytation 1. Visit our Applications page to see more.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!

Features

 

   Digital microscopy and multi-mode detection

 
 
 High quality, high performance at an affordable price

Collect both phenotypic data and quantitative data from one instrument. Image and data analysis are provided with Gen5 software for all applications.

 

   Easy imaging workflow automation

 
 

BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.

Four imaging modes, multiple imaging processes
 

   Fluorescence and high contrast brightfield imaging
 

 
 
 Label-free cell counting

The powerful imaging module in Cytation 1 offers four color channels plus brightfield, from 1.25x to 60x to capture large regions of interest or intracellular details. More than 15 filter/LED cubes are available, supporting a broad range of imaging applications.

 

  Affordable automation

 
 

Cytation 1 has an automated XY stage, autofocus, auto exposure and auto LED intensity for efficient capture of high quality images. The automated features in Cytation 1 and Gen5 make it easy for you to find the region of interest, then quickly find the best settings to capture the images. Gen5 software is simple to use, without extensive training.

Automated digital microscopy to 100x

More than 20 filter/LED color cubes

 
 
Four imaging modes, multiple imaging processes

BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.

 

  Cell friendly design

 
 

Live cell assays require an optimal environment. Cytation 1 has temperature control and shaking, plus CO2 / O2 control, to facilitate longer-term kinetic studies.

Automated digital microscopy to 100x

   High performance and convenience

 
 
Four imaging modes, multiple imaging processes

To optimize multi-mode detection, Cytation 1 has high performance filters for fluorescence and monochromator-based UV-Vis absorbance for convenient wavelength selection in 1 nm increments.

 

  Peltier Cooling for environmental stability

 
 

The Peltier Cooling Module promotes a rapid interior cool down after incubated processes, to allow efficient switching between multiple applications without unwanted temperature influences. For temperature sensitive assays typically run at ambient, or room temperature, the Cooling Module maintains environmental stability within Cytation, allowing < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation.

Automated digital microscopy to 100x

Product Reviews

Customer Spotlight

MimetasTime to Accelerate the Pace of Neurobiology Research

25-March-20

Differentiating a stem cell into a neuron or glial cell is typically a time-consuming bottleneck in human neurological dysfunction research. On top of this, precisely controlling the purity of sub-types in a mixed neuron population can be a complex task, yet is critical to improve assay reproducibility and eliminate unwanted cell types that may negatively impact results.

 


 

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Certifications

Agilent Transcreener - TR FRET Validated Transcreener - FI Validated Transcreener  - Far Red FP DLReady HTRF
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