BioTek Cytation 1 cell imaging multimode reader combines fluorescence and high contrast brightfield imaging with conventional multimode detection. This affordable imaging reader delivers performance and economy compared to other digital microscopy systems. The BioTek Cytation 1 design provides both quantitative phenotypic cellular data with well-based quantitative data. The multimode detection module has high sensitivity filter-based fluorescence and a monochromator for UV-Vis absorbance. Temperature control and shaking are standard. CO2/O2 control and reagent injectors are available. Gen5 software simplifies image capture and plate reading.
- Affordable, patented, quantitative digital microscopy and optional multi-mode microplate detection
- Augmented Microscopy using Gen5 software for seamless image capture to quantitative publication ready data with no extensive training required
- Fluorescence and high contrast brightfield imaging from 1.25x to 60x to capture large regions of interest or intracellular details
- Affordable automation: automated XY stage, focus, exposure, image capture and LED intensity
- More than 20 imaging filter/LED cube colors reach many applications
- Cell friendly design: 4-Zone incubation to 45°C with Condensation Control, CO2/O2 control, angled injectors and lidded plate handling
- High performance filter-based fluorescence detection with convenience of monochromator based UV-Vis absorbance
- Peltier Cooling Module maintains environmental stability for uncompromised assay integrity
Click here to view our Image Gallery of images captured with the Cytation 1.
Testimonials
Seahorse XF Imaging and Normalization System
Cytation 1 is a key component of Agilent Technologies’ Seahorse XF Imaging and Normalization System. XFe Analyzers simultaneously measure the two major cellular energy-producing pathways, mitochondrial respiration and glycolysis, in live cells and in real time. The integrated solution with Cytation 1 combines cellular analysis and imaging for an intuitive workflow to streamline XF data interpretation.applications
Live Cell Proliferation and Viability Application Guide

The Scratch Assay Starter Kit automates cell migration and wound healing assays

The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).
These are just a few examples of the many important applications for Cytation 1. Visit our Applications page to see more.
BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!
Features
Easy imaging workflow automation
BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.
Fluorescence and high contrast brightfield imaging
The powerful imaging module in Cytation 1 offers four color channels plus brightfield, from 1.25x to 60x to capture large regions of interest or intracellular details. More than 15 filter/LED cubes are available, supporting a broad range of imaging applications.
Affordable automation
Cytation 1 has an automated XY stage, autofocus, auto exposure and auto LED intensity for efficient capture of high quality images. The automated features in Cytation 1 and Gen5 make it easy for you to find the region of interest, then quickly find the best settings to capture the images. Gen5 software is simple to use, without extensive training.

More than 20 filter/LED color cubes
BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.
Cell friendly design
Live cell assays require an optimal environment. Cytation 1 has temperature control and shaking, plus CO2 / O2 control, to facilitate longer-term kinetic studies.

Peltier Cooling for environmental stability
The Peltier Cooling Module promotes a rapid interior cool down after incubated processes, to allow efficient switching between multiple applications without unwanted temperature influences. For temperature sensitive assays typically run at ambient, or room temperature, the Cooling Module maintains environmental stability within Cytation, allowing < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation.

Customer Spotlight

25-March-20
Differentiating a stem cell into a neuron or glial cell is typically a time-consuming bottleneck in human neurological dysfunction research. On top of this, precisely controlling the purity of sub-types in a mixed neuron population can be a complex task, yet is critical to improve assay reproducibility and eliminate unwanted cell types that may negatively impact results.