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Lionheart LXAutomated Microscope

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The Lionheart LX automated microscope is designed for affordability, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular free hardware design is more comfortable to use and reduces ergonomic distress in operators. Along with Gen5 software, Lionheart LX enables Augmented Microscopy, which fully automates image capture, processing, and analysis. Operation is easy, load samples, start a run and gather publication-ready images and quantitative data. Lionheart LX can increase your research output and reduce time and costs.



With its comprehensive feature set, Lionheart LX automates many microscopy workflows, including:

Scratch Assay Starter Kit
High contrast brightfield for label-free cell counting.

These are just a few examples of the many important applications for Lionheart LX. Visit our Applications page to see more.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!



   Automated digital microscopy

 Automated digital microscopy to 100x

Lionheart LX has an automated 6-objective turret and can contain up to 4 LED/filter cubes for multi-channel image capture. The precise automated stage and autofocus, auto exposure and other Gen5 software features enable simple, automated image capture and analysis.


Multiple imaging modes and processes


With brightfield, color brightfield and fluorescence imaging in four channels – and more than twenty available filter/LED cubes, Lionheart LX offers fantastic versatility that isn’t limited to image capture. Imaging processes from z-stacking to z-projection, montage collection to stitching; plus analysis from label-free cell counting to mitochrondrial membrane potential are all accomplished with Lionheart LX and Gen5 software.


   Easy imaging workflow automation

 easy imaging

BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.


   Instant cell count and confluence measurement


Get cell counts and confluence calculations directly on the live camera feed. Gen5 software controls Lionheart LX to quickly find the region of interest and display counts onscreen without needing to capture the image. The quick analyze function works for samples in a variety of labware.


   More than 20 filter/LED color cubes

Four imaging modes, multiple imaging processes

BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.


   Quick installation and easy setup


Lionheart LX integrates all microscopy hardware into a very compact footprint, saving valuable bench space. The easily accessible objectives and LED/filter cubes make setup and operation fast and simple, with a minimal learning curve.

Lionheart LX dimensions

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