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Lionheart LXAutomated Microscope

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The Lionheart LX automated microscope is designed for affordability, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular free hardware design is more comfortable to use and reduces ergonomic distress in operators.

Along with Gen5 software, Lionheart LX enables Augmented Microscopy, which fully automates image capture, processing, and analysis. Operation is easy, load samples, start a run and gather publication-ready images and quantitative data. Lionheart LX can increase your research output and reduce time and costs.

Click here to view our Image Gallery of images captured with the Lionheart LX.


With its comprehensive feature set, Lionheart LX automates many microscopy workflows, including:

Scratch Assay Starter Kit
High contrast brightfield for label-free cell counting.

These are just a few examples of the many important applications for Lionheart LX. Visit our Applications page to see more.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!


green box Small benchtop footprint

Small benchtop footprint

The Lionheart LX has a small footprint and is easily installed on a standard lab bench with no need for a dedicated darkroom.

green box High-quality optical components

High-quality optical components

Lionheart LX uses high-quality Olympus objectives and Semrock filters, which drive excellent image quality and performance.

green box Wide field of view

Wide field of view

The wide field of view (WFOV) camera provides fast, automated imaging in microplates and slides. Large tissue sections (left) and microplates (right) can be imaged from low to high magnifications. 

green box Augmented microscopy

Augmented microscopy

Lionheart LX and Gen5 work together for a fully automated capture, process, analyze, and publish workflow. Unlike many other microscopes, the Lionheart platform provides users with complete microscopy workflow control, all the way from image capture to publishable results.

green box Open-stage design

Open-stage design

The open design of the Lionheart LX stage allows researchers to work with microfluidic devices.

green box Autofocus customized for your sample types and labware

Autofocus customized for your sample types and labware

A. Image-based autofocus is available on all Agilent BioTek imaging systems. It focuses on the plane of highest contrast in the sample, including “shifting” biology within the well.
B. Laser autofocus uses the same focal offset from well to well and is typically faster. It works with dim fluorophores and helps prevent phototoxicity and photobleaching. Laser autofocus also offers better reproducibility and higher accuracy during long-term kinetic imaging.


For Research Use Only. Not for use in diagnostic procedures.

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