Lionheart FXAutomated Microscope
Technical Details
General
| Microplate types | 6- to 1536-well plates |
| Other labware supported |
Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Support for labware up to 1.5" tall |
| Temperature control | To 40 °C; uniformity +/- 0.5 °C at 37 °C |
| Software |
Gen5 Microplate Reader and Imager Software (included) Optional software: Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure, Gen5 Secure Image+, Gen5 Secure Image Prime: 21 CFR Part 11 compliant features Auto ROI module Spot Count module Cell Count & Viability app |
| CO2 and O2 control (option) |
Range: 0 - 20% (CO2) 1 - 19% (O2) Control Resolution: +0.1% (CO2 and O2) Stability: +0.2% at 5% (CO2) +0.2% at 1% (O2) Models for both CO2/O2 or CO2 only are available |
| Environmental control cover | Top cover for light tight imaging and incubation control (option) |
| X/Y Stage resolution | Lead screw driven stage with 0.1 µm resolution |
| Humidity control | Humidity chamber with rapid gas recharge (option) |
| Imaging - Field of view (WFOV) mode | 3.42 x 3.42 mm at 4x magnification |
Imaging - Widefield Microscope
| Imaging mode | Fluorescence, contrast brightfield, high contrast brightfield, color brightfield, and phase contrast |
| Imaging method | Single color, multi-color, montage, time lapse, z-stacking, z-stack montage |
| Light source | User-replaceable LEDs (available wavelengths: 365 nm, 390 nm, 405nm 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) |
| Image processing | Z-projection, digital phase contrast, stitching |
| Camera | Sony CMOS 16-bit grayscale camera |
| Camera binning | Optional 2x2 binning for focus and/or image capture |
| Camera exposure range | 5 milliseconds to 4 seconds |
| Image outputs available |
Raw Images: 16-bit TIFF Saved Images: TIF, JPG, BMP, PNG, EMF, GIF Movies: MP4, WMV |
| Objective capacity | 6-position automated turret for user-replaceable objectives |
| Objectives available |
Air: 1.25x, 2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x Oil: 60x, 100x |
| Phase objectives available | 4x, 10x, 20x, 40x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC |
| Automated functions | Autofocus, user-trained autofocus, auto exposure, auto-LED intensity |
| Autofocus method |
Image-based autofocus Laser autofocus option |
| Image collection rate |
Single well fastest frame rate capture: *Full Resolution: up to 10 frames per second for single color images *2x2 Binning: up to 20 frames per second for single color images |
| Microscope stage control |
Gen5 Software Control Optional joystick controller |
| Image flatness WFOV mode |
<20% CV for blank uncorrected brightfield images <5% CV for corrected brightfield images, when using > 4X magnification |
| Image capture rate |
Laser autofocus, 20x, 100 ms exposure, 96 wells: 4 min, 4 sec Software autofocus, 20x, 100 ms exposure, 96 wells: 7 min, 3 sec |
Reagent Injectors (option)
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri and cell culture dishes, chamber slides |
| Dead volume | <1.65 mL with back flush |
| Dispense tip options |
Aligned tip - aligned with optical path for dispensing for fast kinetic assays Offset tip - dispensing is offset from the optical path |
| Dispense volume | 5 - 1000 µL in 1 µL increments |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 - 200 µL |
Physical Characteristics
| Power | External 250W (minimum), 24VDC power supply compatible with 100-240 VAC @50-60Hz. |
| Dimensions |
With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm) |
| Weight |
With top cover: 58 lbs (26.3 kg) Without top cover: 51 lbs (23.1kg) |
Regulatory
| Regulatory | CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. |