product design

Lionheart FXAutomated Microscope

Technical Details

General

Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Temperature control To 40 °C; uniformity +/- 0.5 °C at 37 °C
Software Gen5 Microplate Reader and Imager Software (included)
Optional software:
Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure, Gen5 Secure Image+, Gen5 Secure Image Prime: 21 CFR Part 11 compliant features
Auto ROI module
Spot Count module
Cell Count & Viability app
CO2 and O2 control (option) Range: 0 - 20% (CO2)
1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% (CO2)
+0.2% at 1% (O2)
Models for both CO2/O2 or CO2 only are available
Environmental control cover Top cover for light tight imaging and incubation control (option)
X/Y Stage resolution Lead screw driven stage with 0.1 µm resolution
Humidity control Humidity chamber with rapid gas recharge (option)
Imaging - Field of view (WFOV) mode 3.42 x 3.42 mm at 4x magnification

Imaging - Widefield Microscope

Imaging mode Fluorescence, contrast brightfield, high contrast brightfield, color brightfield, and phase contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking, z-stack montage
Light source User-replaceable LEDs (available wavelengths: 365 nm, 390 nm, 405nm 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Image processing Z-projection, digital phase contrast, stitching
Camera Sony CMOS 16-bit grayscale camera
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
Movies: MP4, WMV
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available Air: 1.25x, 2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Oil: 60x, 100x
Phase objectives available 4x, 10x, 20x, 40x      
Image filter cube capacity 4 user-replaceable fluorescence cubes
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller
Image flatness WFOV mode <20% CV for blank uncorrected brightfield images
<5% CV for corrected brightfield images, when using > 4X magnification
Image capture rate Laser autofocus, 20x, 100 ms exposure, 96 wells: 4 min, 4 sec
Software autofocus, 20x, 100 ms exposure, 96 wells: 7 min, 3 sec

Reagent Injectors (option)

Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume <1.65 mL with back flush
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays
Offset tip - dispensing is offset from the optical path
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL

Physical Characteristics

Power External 250W (minimum), 24VDC power supply compatible with 100-240 VAC @50-60Hz.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)
Weight With top cover: 58 lbs (26.3 kg)
Without top cover: 51 lbs (23.1kg)

Regulatory

Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.
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