Lionheart FXAutomated Microscope
Technical Details
General
| Microplate types | 6- to 1536-well plates |
| Other labware supported |
Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Support for labware up to 1.5" tall |
| Temperature control | Incubation to 40 °C with optional environmental control cover |
| Software |
Gen5 Microplate Reader and Imager Software included Gen5 Image+ and Image Prime Software available for advanced image analysis (option) Gen5 Secure for 21 CFR Part 11 Compliance (option) |
| CO2 and O2 control (option) | 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller |
| Environmental control cover | Top cover for light tight imaging and incubation control (option) |
| X/Y Stage resolution | Lead screw driven stage with 0.1 µm resolution |
| Humidity control | Humidity chamber with rapid gas recharge (option) |
Imaging - Widefield Microscope
| Imaging mode | Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast |
| Imaging method | Single color, multi-color, montage, time lapse, Z-stacking, burst mode |
| Light source | High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) |
| Camera | 16-bit grayscale Sony CMOS |
| Camera binning | Optional 2x2 binning for focus and/or image capture |
| Camera exposure range | 5 milliseconds to 4 seconds |
| Image outputs available |
Raw Images: 16-bit TIFF Saved Images: TIF, JPG, BMP, PNG, EMF, GIF Movies: MP4, WMV |
| Objective capacity | 6 onboard, user-replaceable objectives |
| Objectives available |
Fluorescence: Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70 Oil: 60x, NA:1.42; 100x, NA: 1.40 Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60 |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 |
| Automated functions | Autofocus, user-trained autofocus, auto exposure, auto-LED intensity |
| Autofocus method |
Image-based autofocus Laser autofocus option |
| Image collection rate |
Single well fastest frame rate capture: *Full Resolution: up to 10 frames per second for single color images *2x2 Binning: up to 20 frames per second for single color images |
| Microscope stage control |
Gen5 Software Control Optional joystick controller |
Reagent Injectors (option)
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri and cell culture dishes, chamber slides |
| Dead volume | <1.65 mL with back flush |
| Dispense tip options |
Aligned tip - aligned with optical path for dispensing for fast kinetic assays Offset tip - dispensing is offset from the optical path |
| Dispense volume | 5 - 1000 µL in 1 µL increments |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 - 200 µL |
Physical Characteristics
| Power | 250 Watts maximum consumption. |
| Dimensions |
With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm) With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm) |
| Weight |
Without environmental control cover: 51 lbs (23.1 kg) With environmental control cover: 58 lbs (26.3 kg) |
Regulatory
| Regulatory | CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. |