Lionheart FXAutomated Microscope
Lionheart™ FX Automated Microscope is a compact, inclusive microscopy system for a broad range of imaging workflows. It offers up to 60x air; 60x and 100x oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. An optional environment control cover provides incubation to 40°C and effective containment for CO2/O2 control, a humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays. Lionheart FX and Gen5 together comprise Augmented Microscopy™ - the automation of image capture, processing, analysis and development of publication-ready images and data.
- Automated digital microscopy up to 60x air; 60x and 100x oil immersion
- Fluorescence, brightfield, color brightfield and phase contrast imaging
- Label-free cell counting for cell proliferation studies
- Live cell assay support with environmental controls
- More than 20 imaging filter/LED cube colors reach many applications
- Compact design for quick installation and setup
What's New
The Scratch Assay Starter Kit automates cell migration and wound healing assays, used along with BioTek’s Lionheart FX Automated Microscope or Cytation 5 Cell Imaging Multi-Mode Reader.
applications
Lionheart FX is designed for easy implementation of a broad range of fixed and live cell assay workflows. BioTek’s concept of Augmented Microscopy™ brings automated image capture, processing, analysis and publication-ready images and data to the benchtop – for many applications, including these important areas:
- Cell migration and invasion assays
- Live cell imaging
- High resolution microscopy
- Immunofluorescence
- Phenotypic assays
- Histology
The Scratch Assay Starter Kit automates cell migration and wound healing assays:
AutoScratch automatically makes consistent, repeatable scratch wounds.
The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).
AutoScratch creates scratch wounds with excellent performance, compared to manual methods.
Adding 10 µM ATP activates HeLa endogenously expressed P2Y receptor
Rat kidney mitosis, prophase at 60x.
Visit our Applications pages to learn more about all of the critical applications enabled with Lionheart FX.
BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!
Features
Automated digital microscopy up to 100x
Lionheart FX with Augmented Microscopy automates your imaging workflow to yield amazing images and powerful data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.
Four imaging modes, multiple imaging processes
With brightfield, color brightfield, phase contrast and fluorescence imaging in four channels – and nearly twenty available filter/LED cubes, Lionheart FX offers fantastic versatility that isn’t limited to image capture. Follow your imaging processes through z-stacking to z-projection, montage collection to stitching and digital phase contrast to analysis, all one hardware and software platform.
Label-free cell counting
It’s fast and simple to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.
Kinetic live cell assay support
Several options are available to support live cell assays with Lionheart FX, including an environmental cover to maintain temperature and gas levels within the system. Long-term live cell kinetics are supported, using the unique humidity chamber that fits on the stage itself, keeping the live cell in a completely nurturing environment. A dual reagent injector provides quick inject/image capability for observing fast reactions.
More than 20 imaging filter/LED cubes
BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.
Quick installation and easy setup
Fifteen minutes is all it takes to install and set up Lionheart FX for image capture and analysis. The filter/LED cubes and objectives are accessed from the front panel, and tool-free connections for injectors, gas control and the environmental cover add to the easy installation. Its small size takes up minimal benchtop space.
Customer Spotlight
Cytation and Lionheart Help to Identify cFLIPL as a Key Regulator of Cell Death and Inflammation
21-April-20
At Tufts University’s Graduate School of Biomedical Sciences, Dr. Alexander Poltorak seeks to understand how the host immune system responds when facing a pathogen. If the response is too strong or too weak, the results can be devastating. “If we look at the SARS-CoV-2 virus impacting the world right now, many deaths aren’t a direct result of the coronavirus itself,” he says. “Instead, it’s a complication of the infection; a violent, unbalanced response from the immune system resulting in many different pathological processes or attacks taking place in the lungs.”
Certifications
