product design

Lionheart FXAutomated Microscope

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The Agilent BioTek Lionheart FX automated microscope is a compact system for a broad range of imaging workflows. It offers up to 60x air, in addition to 60x and 100x oil immersion magnification. Combined with fluorescence, brightfield, color brightfield, and phase contrast imaging channels, the imager provides maximum application reach. An optional environment control cover provides incubation to 40 °C, in addition to effective containment for CO2/O2 control to enable performance of cell-based assays. A humidity chamber can be added to optimize conditions for long-term live cell imaging applications. An available dual reagent injector also facilitates rapid kinetic assays. 
 


Click here to view our Image Gallery of images captured with the Lionheart FX.

applications

Lionheart FX is designed for easy implementation of a broad range of fixed and live cell assay workflows. BioTek’s concept of Augmented Microscopy brings automated image capture, processing, analysis and publication-ready images and data to the benchtop – for many applications, including these important areas:

Live Cell Proliferation and Viability Application Guide
Live Cell Proliferation and Viability

 

The Scratch Assay Starter Kit automates cell migration and wound healing assays:

AutoScratch automatically makes consistent, repeatable scratch wounds

AutoScratch automatically makes consistent, repeatable scratch wounds.

The Scratch Assay App automatically calculates % confluence,  wound width and maximum healing rate (max V)

The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).

The Scratch Assay Starter kit enables automated scratch wound assays with excellent performance, compared to manual methods.

AutoScratch creates scratch wounds with excellent performance, compared to manual methods.

 

Adding 10 µM ATP activates HeLa endogenously expressed P2Y receptor

Adding 10 µM ATP activates HeLa endogenously expressed P2Y receptor


Rat kidney mitosis

Rat kidney mitosis, prophase at 60x.
 

 

Visit our Applications pages to learn more about all of the critical applications enabled with Lionheart FX.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!

Features

green box Small benchtop footprint

Small benchtop footprint

The Lionheart FX has a small footprint and is easily installed on a standard lab bench with no need for a dedicated darkroom.


green box High-quality optical components

High-quality optical components

Lionheart FX uses high-quality Olympus objectives and Semrock filters, which drive excellent image quality and performance.


green box Wide field of view

Wide field of view

The wide field of view (WFOV) camera provides fast, automated imaging in microplates and slides. Large tissue sections (left) and microplates (right) can be imaged from low to high magnifications. 


green box Augmented microscopy

Augmented microscopy

When paired with Gen5 imaging software, the Lionheart FX provides a unique fully automated capture, process, analyze and publish workflow.


green box Environmental controls for live cell imaging 

 Environmental controls for live cell imaging 

Successful live cell kinetic imaging relies on a consistent environment, including temperature, CO2/O2, and humidity control. Lionheart FX provides the perfect environment to grow and analyze live cells over time. Powerful movie maker and kinetic analysis software tools allow visualization and analysis of time-lapse experiments.


green box Open-stage design

Open-stage design

The open design of the Lionheart FX stage allows researchers to work with microfluidic devices.


green box Autofocus customized for your sample types and labware

Autofocus customized for your sample types and labware

A. Image-based autofocus is available on all Agilent BioTek imaging systems. It focuses on the plane of highest contrast in the sample, including “shifting” biology within the well.
B. The BioTek patented autofocus method uses the same focal offset from a laser identified interface unique to each well or area to be imaged. It is typically faster, works with dim fluorophores, and helps prevent phototoxicity and photobleaching. Laser autofocus also offers better reproducibility and higher accuracy during long term kinetic imaging.

 


green box Dual-reagent injector module

 Dual-reagent injector module

The dual-reagent injector module for Lionheart FX allows fast cellular reactions to be imaged after the addition of a reagent.



For Research Use Only. Not for use in diagnostic procedures.

Product Reviews

Customer Spotlight

Boston UniversityTaking a Close Look at the Basics of Life with the Help of Lionheart FX

28-May-21

For the curious at heart, science represents an endless reservoir of questions to ask. But sometimes in the quest for knowledge, researchers can overlook the most fundamental yet complex inquiries. This is where Dr. Joseph Larkin aims to make his mark.

At the convergence of Boston University’s Biology and Physics Departments and also as part of the University’s Biological Design Center in Boston, Massachusetts, Dr. Larkin’s new laboratory poses the questions, “How – and why – do living things proactively and reactively interact with each other and their environment to perpetuate?”

 

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Certifications

ONIX
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