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Synergy Neo2 Hybrid Multimode Reader

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BioTek Synergy Neo2 multimode microplate reader features patented Hybrid Technology, with independent optical paths that ensure excellent performance in all detection modes. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF, and up to 4 PMTs provide ultrafast measurements. Advanced environmental controls, including CO2/O2 control, incubation to 70 °C, and variable shaking support live cell assays. BioStack Neo delivers walk-away automation, high throughput, barcode-labeled filter cubes to streamline workflows and limit errors. Powerful Gen5 software provides complete reader control, powerful data analysis, and LIMS integration.



Synergy Neo2 is well suited for many applications requiring a variety of detection modes. Here we list some of the most important applications for this fast, high performance microplate reader.

hTNFα analyte
hTNFα analyte titrations
hTNFα analyte
Nucleic acid quantification using PicoGreen®
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate

These are just a few examples of the many important applications for Synergy Neo2. Visit our Applications page to see more.


BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!

Automate Your Workflows

Expand your workflow automation by integrating the Synergy Neo2 with BioTek’s range of robotics, including the BenchCel Microplate Handler, BioStack Microplate Stacker and BioSpa Automated Incubator. See this Tech Note describing benchtop ELISA automation with the BenchCel, EL406 and Synergy Neo2.


RED BOX  BioTek’s fastest, highest-performing multimode reader

BioTek’s fastest, highest-performing multi-mode reader

RED BOX  Hybrid plate reader: Flexibility and performance

Hybrid plate reader: Flexibility and performance
With its patented combination of monochromator and filter optics, Synergy Neo2 is an advanced plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab.
Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser.

RED BOX  Variable bandwidth for sensitivity and specificity

Condensation and evaporation control
The Synergy Neo2 optics use a quad monochromator design, with variable bandwidth settings between 3 nm and 50 nm, in 1 nm increments. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, reducing signal crosstalk and enhancing assay performance.

RED BOX  Dual PMT = Fast Reading Speed

Dual PMT = Fast Reading Speed
Two PMTs: Synergy Neo2 takes two measurements simultaneously. Other systems have to perform FP, FRET and TR-FRET measurements one at a time, which considerably lengthens the time to results.

RED BOX  Two lasers: For TRF, TR-FRET and Alpha

Two lasers: For TRF, TR-FRET and Alpha
Alpha and TRF assays benefit from the increased sensitivity and fast reading speeds enabled by a laser-based system.

RED BOX  Process plates 30% faster with BioStack Neo

Process plates 30% faster with BioStack Neo
Synergy Neo2's dedicated stacker is the fastest "plate server" in the world. Its dual plate carrier enables it to process plates about 30% faster than other stackers on the market. This leads to significant compounding time savings. For example, over the course of a year at 50 plates a day, the total time savings is nearly 40 hours.

RED BOX  Environmental controls for cell-based assays

Environmental controls for cell-based assays
Temperature control to 70 °C, CO2 / O2  control,  and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays.

RED BOX  Microvolume analysis with Take3 plate

Micro-volume analysis with Take3 plate
Synergy Neo2 acts as a microvolume analysis system, when the Take3 or Take3 Trio plates are used. With only 2 µL each, run 16 or 48 samples at a time, saving considerable time compared to single-sample devices. Pre-defined ssDNA, dsDNA, RNA and protein protocols provide rapid quantification results.

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Customer Spotlight

Synergy Neo2Detecting the Rhythms of Life with Synergy Readers


Most organisms have circadian clocks, which regulate 24- hour rhythms in a wide variety of activities and behaviors. In humans, the circadian clock regulates sleep onset and influences how much sleep you need. Not surprisingly, changes to these clock factors influence sleep, but also to a wide variety of disorders such as metabolic syndrome, cardiovascular disease, and even cancer. Dr. John Hogenesch, Ohio Eminent Scholar; Professor of Pediatrics in the Divisions of Human Genetics and Immunobiology and the Perinatal Institute; and Deputy Director, Center for Chronobiology at the Cincinnati Children's Hospital Medical Center, in Cincinnati, Ohio, is one of the world’s foremost experts in the molecular mechanisms of circadian rhythms in mammals. His research centers around understanding how biological clocks work and leveraging them to improve human health.



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