Synergy™ Neo2 Multi-Mode Microplate Reader is designed for the screening laboratory, with speed and ultra-high performance. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF and up to 4 PMTs provide ultra-fast measurements with excellent results. For walk-away automation and high throughput, BioStack Neo is the fastest on the market, and the barcode-labeled filter cubes help streamline workflows and limit errors. Synergy Neo2 features BioTek’s patented Hybrid Technology™, with its independent optical paths that ensure uncompromised performance in all detection modes. Advanced environmental controls, including CO2/O2 control, incubation to 65 °C and variable shaking support live cell assays; cell-based detection is optimized with direct bottom illumination. Powerful Gen5 Software offers complete reader control, powerful data analysis, and automation and LIMS integration.
- BioTek’s fastest, highest-performing multi-mode reader
- Hybrid plate reader: Flexibility and performance
- Variable bandwidth for sensitivity and specificity
- Dual PMT = Fast Reading Speed
- Two lasers: For TRF, TR-FRET and Alpha
- Process plates 30% faster with BioStack Neo
- Environmental controls for cell-based assays
- Micro-volume analysis with Take3 plate
Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly. Click here to request your free sample. |
What's New
Special Promo
Purchase a Synergy Neo2 Multi-Mode Microplate Reader and receive a BioStack Neo Microplate Stacker at 40% off.
A Seamless Automated Workflow for Plate Reader Assay Sample Prep
The Agilent Bravo Liquid Handling system works hand-in-hand with BioTek Synergy Multi-Mode Plate Readers to deliver automated sample prep for standardizing and optimizing plate reader assays.
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Cell Metabolism: MitoXpress and pH-Xtra Assays
Download our Application Note to discover how combining MitoXpress and pH-Xtra reagents with BioTek plate readers provide sensitive detection methods, including advanced lifetime (µs) time-resolved fluorescence (TRF), for getting the most meaningful data out of your cell metabolism assays.
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applications
Synergy Neo2 is well suited for many applications requiring a variety of detection modes. Here we list some of the most important applications for this fast, high performance microplate reader.

hTNFα analyte titrations

Nucleic acid quantification using PicoGreen®

Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate
These are just a few examples of the many important applications for Synergy Neo2. Visit our Applications page to see more.
BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!
Automate Your Workflows
Expand your workflow automation by integrating the Synergy Neo2 with BioTek’s range of robotics, including the BenchCel Microplate Handler, BioStack™ Microplate Stacker and BioSpa™ Automated Incubator. See this Tech Note describing benchtop ELISA automation with the BenchCel, EL406 and Synergy Neo2.
Features
BioTek’s fastest, highest-performing multi-mode reader
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Hybrid plate reader: Flexibility and performance
Variable bandwidth for sensitivity and specificity
Dual PMT = Fast Reading Speed
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Two PMTs: Synergy Neo2 takes two measurements simultaneously. Other systems have to perform FP, FRET and TR-FRET measurements one at a time, which considerably lengthens the time to results. |
Two lasers: For TRF, TR-FRET and Alpha
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Alpha and TRF assays benefit from the increased sensitivity and fast reading speeds enabled by a laser-based system. |
Process plates 30% faster with BioStack Neo
Environmental controls for cell-based assays
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Temperature control to 65 °C, CO2 / O2 control, and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays. |
Micro-volume analysis with Take3 plate
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Synergy Neo2 acts as a micro-volume analysis system, when the Take3 or Take3 Trio plates are used. With only 2 µL each, run 16 or 48 samples at a time, saving considerable time compared to single-sample devices. Pre-defined ssDNA, dsDNA, RNA and protein protocols provide rapid quantification results. |
Customer Spotlight

23-January-19
Most organisms have circadian clocks, which regulate 24- hour rhythms in a wide variety of activities and behaviors. In humans, the circadian clock regulates sleep onset and influences how much sleep you need. Not surprisingly, changes to these clock factors influence sleep, but also to a wide variety of disorders such as metabolic syndrome, cardiovascular disease, and even cancer. Dr. John Hogenesch, Ohio Eminent Scholar; Professor of Pediatrics in the Divisions of Human Genetics and Immunobiology and the Perinatal Institute; and Deputy Director, Center for Chronobiology at the Cincinnati Children's Hospital Medical Center, in Cincinnati, Ohio, is one of the world’s foremost experts in the molecular mechanisms of circadian rhythms in mammals. His research centers around understanding how biological clocks work and leveraging them to improve human health.