BioTek Synergy Neo2 multimode microplate reader features patented Hybrid Technology, with independent optical paths that ensure excellent performance in all detection modes. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF, and up to 4 PMTs provide ultrafast measurements. Advanced environmental controls, including CO2/O2 control, incubation to 70 °C, and variable shaking support live cell assays. BioStack Neo delivers walk-away automation, high throughput, barcode-labeled filter cubes to streamline workflows and limit errors. Powerful Gen5 software provides complete reader control, powerful data analysis, and LIMS integration.
- BioTek’s fastest, highest-performing multimode reader
- Hybrid plate reader: Flexibility and performance
- Variable bandwidth for sensitivity and specificity
- Dual PMT = Fast Reading Speed
- Two lasers: For TRF, TR-FRET and Alpha
- Process plates 30% faster with BioStack Neo
- Environmental controls for cell-based assays
- Microvolume analysis with Take3 plate
applications
Synergy Neo2 is well suited for many applications requiring a variety of detection modes. Here we list some of the most important applications for this fast, high performance microplate reader.

hTNFα analyte titrations

Nucleic acid quantification using PicoGreen®

Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate
These are just a few examples of the many important applications for Synergy Neo2. Visit our Applications page to see more.
BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!
Automate Your Workflows
Expand your workflow automation by integrating the Synergy Neo2 with BioTek’s range of robotics, including the BenchCel Microplate Handler, BioStack Microplate Stacker and BioSpa Automated Incubator. See this Tech Note describing benchtop ELISA automation with the BenchCel, EL406 and Synergy Neo2.
Features
BioTek’s fastest, highest-performing multimode reader
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Hybrid plate reader: Flexibility and performance
Variable bandwidth for sensitivity and specificity
Dual PMT = Fast Reading Speed
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Two PMTs: Synergy Neo2 takes two measurements simultaneously. Other systems have to perform FP, FRET and TR-FRET measurements one at a time, which considerably lengthens the time to results. |
Two lasers: For TRF, TR-FRET and Alpha
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Alpha and TRF assays benefit from the increased sensitivity and fast reading speeds enabled by a laser-based system. |
Process plates 30% faster with BioStack Neo
Environmental controls for cell-based assays
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Temperature control to 70 °C, CO2 / O2 control, and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays. |
Microvolume analysis with Take3 plate
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Synergy Neo2 acts as a microvolume analysis system, when the Take3 or Take3 Trio plates are used. With only 2 µL each, run 16 or 48 samples at a time, saving considerable time compared to single-sample devices. Pre-defined ssDNA, dsDNA, RNA and protein protocols provide rapid quantification results. |
Customer Spotlight

23-January-19
Most organisms have circadian clocks, which regulate 24- hour rhythms in a wide variety of activities and behaviors. In humans, the circadian clock regulates sleep onset and influences how much sleep you need. Not surprisingly, changes to these clock factors influence sleep, but also to a wide variety of disorders such as metabolic syndrome, cardiovascular disease, and even cancer. Dr. John Hogenesch, Ohio Eminent Scholar; Professor of Pediatrics in the Divisions of Human Genetics and Immunobiology and the Perinatal Institute; and Deputy Director, Center for Chronobiology at the Cincinnati Children's Hospital Medical Center, in Cincinnati, Ohio, is one of the world’s foremost experts in the molecular mechanisms of circadian rhythms in mammals. His research centers around understanding how biological clocks work and leveraging them to improve human health.