Synergy HTX Multi-Mode Reader
Technical Details
General
| Detection modes |
UV-Vis absorbance Fluorescence intensity Luminescence Time-resolved fluorescence (secondary mode) Alpha |
| Read methods | End point, kinetic, spectral scanning and well area scanning |
| Microplate types | 6- to 384-well plates |
| Other labware supported |
PCR plates, Petri and cell culture dishes Take3 Micro-Volume Plates |
| Temperature control |
4-Zone™ incubation to 50 °C with Condensation Control™ +0.2 °C at 37 °C |
| Shaking | Linear, orbital |
| Software |
Gen5™ Microplate Reader and Imager Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible |
Absorbance
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 200 - 999 nm, 1 nm increments |
| Monochromator bandwidth | 2.4 nm |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy |
<1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) |
96 wells: 14 seconds 384 wells: 26 seconds |
Fluorescence Intensity
| Light source |
Tungsten halogen Xenon flash (option) |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 300 - 700 nm (200 - 850 nm option) |
| Dynamic range | >6 decades |
| Sensitivity | Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate) |
| Reading speed (kinetic) |
96 wells: 31 seconds 384 wells: 80 seconds |
Luminescence
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity |
10 amol ATP (flash) - Lum and Abs / Lum configurations 30 amol ATP (flash) - Multi-mode configurations |
Time-Resolved Fluorescence
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Monochromator |
Alpha Detection
| Light source | Tungsten halogen |
| Detector | PMT |
| Wavelength selection | Filters |
| Sensitivity | 300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate |
| Read speed | 2 minute (96-well plate) |
Reagent Dispensers
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5-1000 µL in 1 µL increments |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 - 200 µL |
Physical Characteristics
| Connectivity | 1 USB, 1 RS232 for external PC control |
| Power | 100-240 VAC, 50/60 Hz |
| Dimensions |
16"W x 15"D x 10"H (40.6 x 38 x 25.4 cm) |
| Weight | 40 lbs (18 kg) |
Regulatory
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |