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Synergy HTX Multimode Reader

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The BioTek Synergy HTX is a compact, economical multimode reader for 6- to 384-well microplates and Take3 microvolume plates. The unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence, and AlphaScreen/AlphaLISA workflows. With incubation and shaking, plus a dual reagent injector module, the system will meet all your assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 software for data collection, analysis, exporting, and reporting.



With UV-Vis absorbance and filter-based fluorescence and luminescence, Synergy HTX combines versatility and performance for many key endpoint and kinetic applications.
Electron structures of reactive oxygen curve (AUC) species, detectable using Synergy HTX
Electron structures of reactive oxygen curve (ROS) species, detectable using Synergy HTX
Area under the calculated by Gen5 for an ORAC assay
Gen5 calculated area under the curve (AUC) for an ORAC assay.
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate

These are just a few examples of the many important applications for Synergy HTX. Visit our Applications page to see more.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!


Great flexibility at a low price

Affordability doesn’t mean compromising on performance with the Synergy HTX. Its hardware design and Gen5-driven functionality address a wide range of multi-mode applications without costly and often unused options.


Monochromator and filter optics

Synergy HTX provides great sensitivity with its filter-based fluorescence optics, and the monochromator-based UV-Vis absorbance optics offers 200 nm – 999 nm measurements without requiring filters. The combination of independent optics allows multi-mode measurements without compromise.
Patented Technology


Shaking for assay optimization

Linear and orbital shaking
Assays that require shaking, such as bacterial and yeast growth studies, will benefit from the robust, programmable shaking options in Synergy HTX. Select linear or orbital shaking, with programmable speed and duration.

Excellent Alpha assay performance

The sensitive fluorescence optics in Synergy HTX provide great performance for many applications including important AlphaScreen and AlphaLISA assays.
AlphaScreen® and AlphaLISA®

Fast inject/read assays

Dual reagent injectors

The available dual reagent injector module with angled tips, enabels fast inject/read operations, which is critical for enzyme kinetics, Dual-Luciferase Reporter and many other assays. Synergy HTX is easily upgradable to add the dual reagent injector.

Product Reviews

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Farias-UdlFronteraTime to move forward with confidence in results


Dr. Jorge Farías studies cellular and molecular mechanisms in fish spermatozoa as part of his role as the Director of Chemical Engineering at the Universidad de La Frontera in Temuco, Chile. Spermatogenesis, which is the development of sperm, is a delicate balance between cell proliferation, differentiation and apoptosis, and disruptions can have profound implications.





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