product design

Cytation Hybrid Multi-Mode Reader

Specifications

General

Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha (Cytation 5)
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates (absorbance only Cytation 1)
Filters: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C (Cytation 1)
4-Zone incubation to 65 °C (Cytation 5) with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2% at 1% O2
Models for both CO2 and O2 or CO2 only are available

Absorbance

Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments (Cytation 5)
200 - 999 nm, 1 nm increments (Cytation 1)
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm) (Cytation 5)
2.4 nm (Cytation 1)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds

Fluorescence Intensity

Light source Xenon flash
Detector PMT for monochromator system (Cytation 5)
PMT
Wavelength selection Quad monochromators (top/bottom) (Cytation 5)
Filters / dichroic mirrors
Wavelength range Monochromators: 250 - 700 nm (850 nm option) (Cytation 5)
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth Variable; from 9 to 50 nm, in 1 nm increments (Cytation 5)
Dynamic range 7 decades
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator (Cytation 5):
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds

Luminescence

Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity Monos: 20 amol ATP (flash) (Cytation 5)
Filters: 10 amol ATP (flash), 100 amol (glow)

Fluorescence Polarization

Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 280 - 700 nm (850 nm option)
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein

Time-Resolved Fluorescence

Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (secondary mode) (Cytation 5)
Filters (top)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Filters: Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate) (Cytation 5)

Alpha Detection

Light source 100 mW 680 nm laser (Cytation 5)
Detector PMT (Cytation 5)
Wavelength selection Filters (top) (Cytation 5)
Sensitivity 100 amol LCK peptide (384-well low volume plate) (Cytation 5)

Reagent Dispensers

Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri dishes
Dead volume 1.1 mL, 100 µL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL

Physical Characteristics

Power 150 Watts to 250 Watts, configuration dependent
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 65 lbs to 80 lb (29 kg to 36.3 kg), configuration dependent

Regulatory

Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

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