product design

Cytation Hybrid Multimode Reader

Watch Video    |    See Product Reviews

The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. Detection modes are available in UV-Vis absorbance, fluorescence, and luminescence detection. Fluorescence microscopy to 60x can be added to expand the range of applications in a single instrument. Gen5 software is included with the system and is designed for uncomplicated data collection and processing for the most complex assays.


Cytation Cell Imaging Multimode Readers are uniquely designed to support applications across automated digital microscopy and conventional multi-mode detection. Here we highlight a few key application areas.
Standard curves compared
Standard curves compared; Cytation 5 and Synergy HTX AlphaLISA assay
Fluorescence polarization
Fluorescence polarization: Human serum albumin and tryptophan
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate

These are just a few examples of the many important applications for Cytation. Visit our Applications page to see more.


BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!



   Modular, upgradable design

 Digital microscopy and multi-mode detection

Although your lab may only be working with multimode workflows now, assay demands may change in the future. Cytation’s modularity offers the ability to add automated digital microscopy to the multi-mode system to adapt as your research requirements change.


Patented Hybrid optics


Cytation 5 features a patented, hybrid optical design, with filter-based fluorescence optics for high transmission and high sensitivity, and monochromator optics for convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization.

hybrid optics

   Variable bandwidth monochromators

Variable bandwidth monochromators optimize fluorophore detection

Cytation multimode configurations can include quad monochromators with variable bandwidths, selectable from 9 nm to 50 nm, in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.


   100 mW Alpha Laser


Laser-based excitation in Cytation 5 offers the power required to get great sensitivity and fast reading speeds, which are critical for Alpha assays.

 Label-free cell counting for cell proliferation studies

   Peltier Cooling for environmental stability

Label-free cell counting

The Peltier Cooling Module facilitates interior cool down after incubated processes, to allow efficient switching between multiple applications without unwanted temperature influences. For temperature sensitive assays typically run at ambient, or room temperature, the Cooling Module maintains environmental stability within Cytation, allowing < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation.

Product Reviews


Transcreener - TR FRET Validated Transcreener - FI Validated Transcreener  - Far Red FP DLReady HTRF