Luminescence is the result of a chemical or biochemical reaction. Luminescence detection is simpler optically than fluorescence detection because luminescence does not require a light source for excitation or optics for selecting discrete excitation wavelengths. A typical luminescence optical system consists of a light-tight reading chamber and a PMT detector. Some plate readers offer filter wheel or tunable wavelength monochromator optical systems for selecting specific luminescent wavelengths.
Figure 1. Sample in the well before the chemical or biochemical reaction.
Figure 2. Reaction causes the sample to emit light that can be measured by a PMT detector.
The ability to select multiple wavelengths allows for detection of assays that contain multiple luminescent reporters, the development of new luminescence assays, as well as a means to optimize signal-to-noise.
Common applications include luciferase -based gene expression assays, as well as cell viability, cytotoxicity, and biorhythm assays based on the luminescent detection of ATP.
Glow Luminescence is available in:
Agilent BioTek Synergy Neo2 Multi-Mode Reader
Agilent BioTek Cytation 7 Cell Imaging Multi-Mode Reader
Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
Agilent BioTek Cytation 1 Cell Imaging Multi-Mode Reader
Agilent BioTek H1 Multi-Mode Reader
Agilent BioTek Synergy HTX Multi-Mode Reader
Agilent BioTek Synergy LX Multi-Mode Reader