Synergy H1 Hybrid Multi-Mode Reader
Technical Details
General
Detection modes |
UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence |
Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
Microplate types | 6- to 384-well plates |
Other labware supported | Take3 Micro-Volume Plates |
Temperature control | 4-Zone incubation to 70 °C ("M2" configurations) or to 45 °C(all other configurations) |
Shaking | Linear, orbital, double orbital |
Software |
Gen5 Microplate Reader and Imager Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
Automation |
BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
CO2 and O2 control (option) |
Range: 0 - 20% (CO2); 1 - 19% (O2) Control Resolution: +0.1% (CO2 and O2) Stability: +0.2% at 5% CO2; +0.2% at 1% O2 Models for both CO2 and O2 or CO2 only are available |
Absorbance
Light source | Xenon flash |
Detector | photodiode |
Wavelength selection | monochromator |
Wavelength range | 230 - 999 nm, 1 nm increments |
Monochromator bandwidth | 4 nm (230-285 nm), 8 nm (>285 nm) |
Dynamic range | 0 - 4.0 OD |
Pathlength correction | yes |
Monochromator wavelength accuracy | ±2 nm |
Monochromator wavelength repeatability | ±0.2 nm |
OD accuracy | <1% at 3.0 OD |
Reading speed (kinetic) |
96 wells: 11 seconds 384 wells: 22 seconds |
Fluorescence Intensity
Light source | Xenon flash |
Detector |
PMT for monochromator system PMT for filter system |
Wavelength selection |
Quad monochromators (top/bottom) Filters (top) |
Wavelength range | Monochromators: 250 - 700 nm (900 nm option) |
Monochromator bandwidth |
Fixed, 16 nm Variable; from 9 to 50 nm, in 1 nm increments ("M2" configurations) |
Dynamic range | 7 decades |
Sensitivity |
Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate) Quad Monochromator: Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
Reading speed (kinetic) |
96 wells: 11 seconds 384 wells: 22 seconds |
Luminescence
Sensitivity |
Monos: 20 amol ATP Filters: 10 amol ATP |
Fluorescence Polarization
Light source | Xenon flash |
Detector | PMT |
Wavelength selection | Filters |
Wavelength range | 280 – 700 nm (850 nm option) |
Sensitivity | 2 mP standard deviation at 1 nm fluorescein |
Time-Resolved Fluorescence
Light source | Xenon flash |
Detector | PMT |
Wavelength selection | Filters (top) |
Sensitivity | <40 fM |
Reagent Dispensers
Supported detection modes | All modes |
Number | 2 syringe pumps |
Supported labware | 6- to 384-well microplates |
Dead volume | 1.1 mL with back flush |
Dispense volume | 5 - 1000 µL in 1 µL increment |
Dispense accuracy | ±1 µL or 2% |
Dispense precision | <2% at 50-200 µL |
Physical Characteristics
Power | 130 Watts maximum consumption. |
Dimensions |
14.75"W 18.25"D 13"H (37.5 x 46.4 x 33 cm) |
Weight | <55 lbs (24.95 kg) |
Regulatory
Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |