Synergy H1 Hybrid Multi-Mode Reader
Technical Details
General
| Detection modes |
UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | 6- to 384-well plates |
| Other labware supported |
Petri and cell culture dishes Take3 Micro-Volume Plates |
| Temperature control |
4-Zone™ incubation to 45 °C with Condensation Control™ +0.2 °C at 37 °C |
| Shaking | Linear, orbital, double orbital |
| Software |
Gen5™ Data Analysis Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation |
BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) |
Range: 0 - 20% (CO2); 1 - 19% (O2) Control Resolution: +0.1% (CO2 and O2) Stability: +0.2% at 5% CO2; +0.2% at 1% O2 Models for both CO2 and O2 or CO2 only are available |
Absorbance
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increments |
| Monochromator bandwidth | 4 nm (230-285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | ±2 nm |
| Monochromator wavelength repeatability | ±0.2 nm |
| OD accuracy |
<1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) |
96 wells: 11 seconds 384 wells: 22 seconds |
Fluorescence Intensity
| Light source | Xenon flash |
| Detector |
PMT for monochromator system PMT for filter system |
| Wavelength selection |
Quad monochromators (top/bottom) Filters (top) |
| Wavelength range |
Monochromators: 250 - 700 nm (850 nm option) Filters: 200 - 700 nm (850 nm option) |
| Monochromator bandwidth | Fixed, 16 nm |
| Dynamic range | 7 decades |
| Sensitivity |
Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate) Quad Monochromator: Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
| Reading speed (kinetic) |
96 wells: 11 seconds 384 wells: 22 seconds |
Luminescence
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity |
Monos: 20 amol ATP (flash) Filters: 10 amol ATP (flash), 100 amol (glow) |
Fluorescence Polarization
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 - 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nm fluorescein |
Time-Resolved Fluorescence
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection |
Quad monochromators (secondary mode) Filters (top) |
| Wavelength range | Filters: 200 - 700 nm (850 nm option) |
| Sensitivity |
Filters: Europium 40 fM (4 amol/well, 384-well plate) Monos: Europium 1200 fM (120 amol/well, 384-well plate) |
Reagent Dispensers
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dispense accuracy | ±1 µL or 2% |
| Dispense precision | <2% at 50-200 µL |
Physical Characteristics
| Power | 130 Watts maximum consumption. |
| Dimensions |
15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm) |
| Weight | 50 lbs (22.5 kg) |
Regulatory
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |