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Synergy H1 Hybrid Multi-Mode Reader

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Synergy™ H1 is a configurable multi-mode microplate reader, with monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers applications versatility and excellent performance in a modular platform to expand as your laboratory’s needs change. Synergy H1 now offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection; the fluorescence bandwidth can be set between 9 nm and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.

What's New

Filter CubePurchase a Synergy or Cytation Reader for use with Agilent MitoXpress or pH-Xtra Assays and receive a free filter cube!


Download our Application Note to discover how combining MitoXpress and pH-Xtra reagents with BioTek plate readers provide sensitive detection methods, including advanced lifetime (µs) time-resolved fluorescence (TRF), for getting the most meaningful data out of your cell metabolism assays.



With quadruple variable bandwidth monochromators and optional gas control and reagent injection, Synergy H1 can be used for a wide range of applications in fluorescence, luminescence, UV-Vis and more.
H1 yeast
Effect of increasing levels of ethanol on yeast growth
HTRF® ratios for AMD3011 and SDF1-alpha
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate

These are just a few examples of the many important applications for Synergy H1. Visit our Applications page to see more.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!


green box Cost effective Hybrid™ multi-mode reader

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Synergy H1 is a versatile plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab. Synergy H1 is synonymous with performance and value.

green box Variable bandwidth monochromators

Variable bandwidth for sensitivity and specificity

Synergy H1 offers quad monochromator optics with variable bandwidth. The bandwidth can be set between 9 nm and 50 nm, in 1 nm increments. Large bandwidths (1) provide increased sensitivity and lower limits of detection. Small bandwidths (2) provide increased specificity when multiple signals are present, reducing crosstalk and enhancing assay performance.

green box Modular and upgradable

Advanced microscopy: Unlimited possibilities

With both monochromator- and filter-based modules, environmental controls and reagent injectors, Synergy H1 is an affordable, upgradable solution to address many critical applications today and well into the future.

green box Micro-volume quantification  Micro-volume analysis with Take3 plate
Enable micro-volume analysis with the Synergy H1, using the Take3 Plate. Measure up to 16 or 48 samples in one run and save a lot of time, compared to single-sample devices. Gen5 has customizable protocols for ssDNA, dsDNA, RNA and protein quantification in 2 µL.

green box Live cell assay friendly environment

Micro-volume analysis with Take3 plate
Temperature control to 45 °C, CO2/O2 control, condensation control and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays.

Product Reviews

Customer Spotlight

SwanseaBiocontrol of Invertebrate Pests Using Natural Agents: Investigating Host-Pathogen Interactions with the BioTek Synergy H1


Biocontrol agents provide a safer alternative to chemical pesticides and insecticides for the agricultural and forestry industries, as well as human health. In this video, Dr Bethany Greenfield, Post-Doctoral Research Assistant in the Biocontrol and Natural Products (BANP) Group at Swansea University, describes her research into the use of Metarhizium anisopliae for the control of mosquitoes. Hear how the BioTek Synergy H1 enables the group to efficiently perform a range of analyses, from protein quantification to measuring insect stress response.




Transcreener - TR FRET Validated Transcreener - FI Validated Transcreener  - Far Red FP DLReady HTRF
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