product design

Synergy H1 Hybrid Multi-Mode Reader


green box Hybrid plate reader: Flexibility and performance

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Synergy H1 is an advanced plate reader
that delivers both the flexibility and performance you need for any microplate assay in your lab.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence

green box Upgradable to meet future application needs

Variable bandwidth for sensitivity and specificity

Synergy H1’s modular design allows you to start with what you need now, and add detection modes, gas control and dual reagent injectors as your laboratory’s workflows evolve.

green box Variable bandwidth for sensitivity and specificity

Advanced microscopy: Unlimited possibilities

Synergy H1 offers quad monochromator optics with variable bandwidth. The bandwidth can be set between 9 nm and 50 nm, in 1 nm increments. Large bandwidths (1) provide increased sensitivity and lower limits of detection. Small bandwidths (2) provide increased specificity when multiple signals are present, reducing crosstalk and enhancing assay performance.

green box Automated z-focus: best performance with all plate types Micro-volume analysis with Take3 plate
(1) Without automated z-focus available, performance at low volumes is affected. (2) With automated z-focus, reading height is precisely adjusted for best performance in all plate types and all volumes.

green box Extended dynamic range

Micro-volume analysis with Take3 plate
Synergy H1 offers an extended dynamic range, which allows detection of signals across a 7 log measurement range. Other systems can measure only small portions of the dynamic range of Synergy H1 using preset gains – this can cause reduced sensitivity on the low end or saturated signals on the high end of the assay signal range.

green box Environmental controls for cell-based assays

Micro-volume analysis with Take3 plate
Temperature control to 45 °C, CO2/O2 control and condensation control and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays.

green box Dual syringe injectors with specialized tips

Micro-volume analysis with Take3 plate
1) The robust precise dual syringe design eliminates the need for regular tubing replacement required by some peristaltic injector designs. Synergy H1 offers two tip types: (2) the straight tips enable vigorous mixing for rapid inject/read assays, and (3) the angled tip option won’t disturb cell layers for applications such as calcium kinetics.

green box  Micro-volume analysis with Take3 plate 

Micro-volume analysis with Take3 plate
Enable micro-volume analysis with the Synergy H1, using the Take3 Plate. Measure up to 16 or 48
samples in one run and save a lot of time, compared to single-sample devices. Gen5 has customizable
protocols for ssDNA, dsDNA, RNA and protein quantification in 2 µL.