Flash luminescence is the result of a chemical or biochemical reaction, triggered by a rapid injection of a catalyst into the microplate well immediately before detection. Flash reactions typically require a rapid dispense and measure sequence on a well-by-well basis. The injectors must be in close proximity to the well that is measured. A typical luminescence optical system consists of a light-tight reading chamber and a PMT detector. Some plate readers offer filter wheel or tunable wavelength monochromator optical systems for selecting specific luminescent wavelengths.
The ability to select multiple wavelengths allows for detection of assays that contain multiple luminescent reporters, the development of new luminescence assays, as well as a means to optimize signal to noise.
Common applications include luciferase -based gene expression assays, as well as cell viability, cytotoxicity, and biorhythm assays based on the luminescent detection of ATP.
Flash Luminescence is available in:
Agilent BioTek Synergy Neo2 Multi-Mode Reader
Agilent BioTek Cytation 7 Cell Imaging Multi-Mode Reader
Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
Agilent BioTek Cytation 1 Cell Imaging Multi-Mode Reader
Agilent BioTek Synergy H1 Multi-Mode Reader
Agilent BioTek Synergy HTX Multi-Mode Reader
For Research Use Only. Not for use in diagnostic procedures.