BioTek: Filtered Luminescence Detection

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  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
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  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
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  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
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  BioTek Announces New Cytation C10 Confocal Imaging Reader
  February 2, 2021 - BioTek Instruments, a part of Agilent, is excited to unveil the Cytation C10, an advanced, automated Confocal Imaging Reader. This versatile and multi-functional automated system is ideal for research laboratories and core facilities looking to increase productivity while reaping high-quality qualitative and quantitative data to support cell-based research. Read more
  
  BioTek Receives Patent for Automated Media Exchange (AMX) Module for the MultiFlo FX Multi-Mode Dispenser
  January 28, 2021 - BioTek Instruments, an Agilent Technologies, Inc., company, announces that it was granted a patent by the United States Patent and Trademark Office for the Automated Media Exchange (AMX) module for the MultiFlo FX Multi-Mode Dispenser. In cell biology workflows, the automated MultiFlo FX with AMX module enables hands-free, gentle, and highly consistent media exchanges and washing in 96- and 384-well microplate formats without disturbing sensitive cells. This promotes viability and long-term differentiation in 3-dimensional (3D) cell culture models as well as effective maintenance of loosely adherent 2-dimensional (2D) cell cultures. Read more
  
  Synergy H1’s Continuously Variable Bandwidth Monochromators Optimize Assay Performance
  July 2, 2020 - BioTek Instruments announces the availability of variable bandwidth monochromators on their modular and versatile Synergy™ H1 Hybrid Multi-Mode Reader. This allows researchers to achieve even greater levels of assay sensitivity and specificity compared to fixed bandwidth systems. Read more
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Home Products Technologies Multi-Mode Detection Technologies Filtered Luminescence

Some luminescence methods call for filtered luminescence. In BioTek’s multi-mode readers, filters can be used to modulate the luminescence emanating from the sample.

Appropriate wavelength filters are positioned between the sample and PMT detector, as shown in this diagram:

diagram

Filtered luminescence is used for assays including: BRET, BRET2, NanoBRET, NanoBIT and ChromaGlo.

Filtered Luminescence is available in:

link

April 10 - May 21, 2021

April 26 - 30, 2021

April 27 - 30, 2021

Filtered Luminescence

Filtered Luminescence is available in: