AlphaScreen is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. This platform provides a nonradioactive, homogeneous assay that has low background and high signal to background ratios. The assay can be easily automated and miniaturized to many well formats. The assay incorporates two sets of beads, donor and acceptor beads, to generate a signal. The donor bead is illuminated at 680 nm. If the acceptor bead is in close proximity to the donor bead, energy is transferred to the acceptor bead, and light is produced in the 520-620 nm range. If an acceptor bead is not close to the donor bead, no energy is transferred, and the donor bead falls back to a ground state.
The Agilent BioTek Synergy Neo2 and Agilent BioTek Cytation 5 have an 100 mW laser excitation source, ideal for providing the excitation energy needed for fast and sensitive Alpha assays. The Agilent BioTek Synergy HTX offers a lower cost option for Alpha assays using a tungsten halogen light source.
Figure 1. Assay principal for AlphaScreen SureFire signal transducers and activators of transcription (STAT)3 assay.
Alpha Detection is available with:
Agilent BioTek Synergy Neo2 multimode reader (laser Alpha)
Agilent BioTek Cytation 5 cell imaging multimode reader (laser Alpha)
Agilent BioTek Synergy HTX multimode reader