product design

Cytation C10 Confocal Imaging Reader

Technical Details

General

Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer), Take3 Micro-volume plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control
Shaking Linear, orbital, double-orbital
Software Gen5 Microplate Reader and Imager Software included
Gen5 Secure for 21 CFR Part 11 compliance (option)
Gen5 Image+ and Image Prime software available for full image analysis (option)
Automation BioSpa 8, BioStack and 3rd party automation compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2), with optional Gas Controller
Models for both CO2 and O2 or CO2 only are available

Imaging - Confocal Microscope

Imaging modes Fluorescence
Image processing Z-projection, digital phase contrast, stitching
Camera Hamamatsu Orca sCMOS, 16-bit grayscale camera or
Sony CMOS 16-bit grayscale camera
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available 20x, 40x, 60x
Image filter cube capacity 4 user-replaceable fluorescence cubes
Imaging filter cubes available CFP, CY5, DAPI, GFP, RFP, TRITC, brightfield
Laser 6-line
Automated functions Autofocus, user-trained autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software Control
Joystick controller (option)
Imaging methods Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Field of view Hamamatsu sCMOS: 0.65 mm + 5% at 20x magnification
Sony CMOS: 0.70 mm + 5% at 20x magnification
Image collection rate Laser autofocus, 0 ms delay, 96 wells: 8 mins, 9 secs
Software autofocus, 0 ms delay, 96 wells: 12 mins, 1 sec

Fluorescence Intensity

Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (top/bottom)
Wavelength range 250 – 700 nm (900 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades
Reading speed (kinetic) 96 wells, sweep mode: 10 seconds
384 wells, sweep mode: 20 second

Luminescence

Wavelength range 300 - 700 nm
Dynamic range >6 decades

Absorbance

Light source Xenon flash
Detector Photodiode
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm
OD accuracy <1% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 10 seconds
384 wells: 20 seconds

Reagent Injectors (option)

Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL, with back flush
Dispense volume 5 – 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates
Dispense accuracy +1 µL or 2%
Dispense precision < 2% at 50 - 200 µL

Physical Characteristics

Power Instrument: External 250W (minimum), 24VDC power supply, compatible with 100-240 VAC @50-60Hz.
Optional 6-channel laser light source: External 250W power supply, compatible with 100- 240VAC @ 50-60Hz.
Optional Hamamatsu scientific camera: External 75W power supply, compatible with 100- 240VAC @ 50-60Hz.
Dimensions 27" W x 18.5" H x 20" D
(68.6 cm x 45.72 cm x 50.8 cm)
Weight 122 lbs (53.3 Kg)

Regulatory

Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Imaging - Widefield Microscope

Imaging mode Fluorescence, phase contrast, color brightfield, user-selectable brightfield/high contrast brightfield
Imaging method Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Image processing Z-projection, digital phase contrast, stitching
Camera Hamamatsu Orca sCMOS, 16-bit grayscale camera or
Sony CMOS 16-bit grayscale camera
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff), 4x,10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes, plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software Control
Joystick controller (option)
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features
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