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Increasingly, life science assays are run on live cells. Some of these assays require control over CO₂ and O₂ concentrations to modulate the environment for pH buffering or to create a hypoxic condition. These assays are traditionally run in flasks in a low-throughput manner, but there is a burgeoning interest in automation of live cell-based assays in microplates for higher throughput and efficiency, therefore there is a need for cell-friendly microplate instrumentation.

The Gas Controller module for Synergy™ H1 allows full control over CO₂ concentrations to regulate the environment for microplate based live cell assays. The Gas Controller and other important cell-friendly features of the Synergy H1, like variable shaking parameters, 4-Zone™ temperature control and a robust mechanical system make them ideal microplate readers for cell-based assays.

Synergy H1 with Gas Controller

Features

• Individual or simultaneous control of CO₂ and O₂ levels
• CO₂ and O₂ Control Resolution ± 0.1 %
• Compact Design
• Infrared CO₂ Sensor
• Zirconium Oxide O₂ Sensor Simple
• Intuitive control panel
• Easy to read gas level indicators and alarm LEDs
• Alarm buzzer on/off

Models

1210008     Gas Controller for CO₂ and O₂ control. For use with Synergy H1 Hybrid Microplate Reader.

1210007     Gas Controller for CO₂ control only. For use with Synergy H1 Hybrid Microplate Reader.

1210011     Calibration Kit for CO₂ Controller.

01503         Calibrated CO₂ Gas Tank

Specifications

Specifications are subject to change

For use with for Synergy™ H1 with silver case. See 1210013 for newer Synergy H1.

The Gas Controller module allows full control over CO₂ and O₂ concentrations to regulate the environment for microplate based live cell assays.

Features

• Individual or simultaneous control of CO₂ and O₂ levels
• CO₂ and O₂ Control Resolution ± 0.1 %
• Compact Design
• Infrared CO₂ Sensor
• Zirconium Oxide O₂ Sensor Simple
• Intuitive control panel
• Easy to read gas level indicators and alarm LEDs
• Alarm buzzer on/off

Models

1210008     Gas Controller for CO₂ and O₂ control. For use with Synergy H1 Hybrid Microplate Reader.

1210007     Gas Controller for CO₂ control only. For use with Synergy H1 Hybrid Microplate Reader.

1210011     Calibration Kit for CO₂ Controller.

01503         Calibrated CO₂ Gas Tank

Specifications

Specifications are subject to change

Increasingly, life science assays are run on live cells. Some of these assays require control over CO₂ and O₂ concentrations to modulate the environment for pH buffering or to create a hypoxic condition. These assays are traditionally run in flasks in a low-throughput manner, but there is a burgeoning interest in automation of live cell-based assays in microplates for higher throughput and efficiency, therefore there is a need for cell-friendly microplate instrumentation.

The Gas Controller allows full control over CO₂ and O₂ concentrations to regulate the environment for microplate based live cell assays.

Increasingly, life science assays are run on live cells. Some of these assays require control over CO₂ and O₂ concentrations to modulate the environment for pH buffering or to create a hypoxic condition. These assays are traditionally run in flasks in a low-throughput manner, but there is a burgeoning interest in automation of live cell-based assays in microplates for higher throughput and efficiency, therefore there is a need for cell-friendly microplate instrumentation.

The Gas Controller allows full control over CO₂ concentrations to regulate the environment for microplate based live cell assays.

Dual Reagent Injector Module includes two syringes, two reagent bottles, holders and inlet tubing. Feedthrough assembly options are required, order separately.

• For use with Lionheart FX, order either aligned tips PN 1450533 for rapid kinetics, or offset tips PN 1450532
• For use with Cytation 5/1, Synergy Neo2 and Synergy H1, order accessory PN 8040038, which includes straight injector tips. Angled tips PN 1320514 can be ordered and exchanged for the straight tips.

The Peltier Cooling Module promotes a rapid interior cool down after incubated processes, to allow efficient switching between multiple applications without unwanted temperature influences. For temperature sensitive assays typically run at ambient, or room temperature, the Cooling Module maintains environmental stability within Cytation, allowing < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation.

Micro-volume quantification is fast and easy with the Take3™ Micro-Volume Plate, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen5 make quantification fast and easy.
 

Features

Low volume 2 µL nucleic acid and protein quantification

It takes only 2 µL of undiluted sample to get rapid results with the Take3 plate. Choose from the 16-sample Take3 or the 48-sample Take3 Trio for higher throughput. Take3 plates are compatible with most of BioTek’s multi-mode readers, imaging multi-mode readers and microplate spectrophotometers.

Pre-programmed protocols in Gen5 Software

Gen5’s pre-programmed dsDNA, ssDNA, RNA and protein (BSA, IgG, lysozyme) measurement protocols make getting direct quantitative results quick and easy. Add sample IDs and define blanks if desired. Endpoint results and selectable spectral scans provide quick visual results and are output in convenient .xls, or .xml formats.

Absorbance and fluorescence detection

The Take3 plates aren’t limited to absorbance detection – they are useful for micro-volume fluorescence quantitation as well – with only 2 µL sample volumes. Compatible with many of BioTek’s multi-mode readers and imaging multi-mode readers.

Easy, wipe-off maintenance

Residue and dust can wreak havoc on micro-volume measurements, so it’s important to keep the sample surfaces super clean. Take3 plates are so easy to clean – simply wipe samples off after measurement with a laboratory wipe.
 

Read 2 µL microspots, BioCells or standard cuvettes

Along with 16 microspots, the Take3 plate can accept a standard 1 cm cuvette or 2 patented BioCells for rapid 1 cm measurements in a BioTek microplate spectrophotometer. The Take3 Trio offers 48 microspots and 2 BioCell positions.

Specifications

See also Take3 Trio.

Micro-volume quantification is fast and easy with the Take3™ Trio Micro-Volume plate, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen5™ make quantification fast and easy.

For use with Synergy™ LX under Gen5 control.

Features

Low volume 2 µL nucleic acid and protein quantification

It takes only 2 µL of undiluted sample to get rapid results with the Take3 plate. Choose from the 16-sample Take3 or the 48-sample Take3 Trio for higher throughput. Take3 plates are compatible with most of BioTek’s multi-mode readers, imaging multi-mode readers and microplate spectrophotometers.

Pre-programmed protocols in Gen5 Software

Gen5’s pre-programmed dsDNA, ssDNA, RNA and protein (BSA, IgG, lysozyme) measurement protocols make getting direct quantitative results quick and easy. Add sample IDs and define blanks if desired. Endpoint results and selectable spectral scans provide quick visual results and are output in convenient .xls, or .xml formats.

Absorbance and fluorescence detection

The Take3 plates aren’t limited to absorbance detection – they are useful for micro-volume fluorescence quantitation as well – with only 2 µL sample volumes. Compatible with many of BioTek’s multi-mode readers and imaging multi-mode readers.

Easy, wipe-off maintenance

Residue and dust can wreak havoc on micro-volume measurements, so it’s important to keep the sample surfaces super clean. Take3 plates are so easy to clean – simply wipe samples off after measurement with a laboratory wipe.
 

Read 2 µL microspots, BioCells or standard cuvettes

Along with 48 microspots, the Take3 Trio plate can accept 2 patented BioCells for rapid 1 cm measurements in a BioTek microplate spectrophotometer.

Specifications

See also Take3.

The Fluorescence Test Plate is designed to replace fluorescent liquid testing often required to check instrument performance to specifications. The plate simplifies the process for conducting fluorescence intensity, fluorescence polarization, and time-resolved fluorescence qualification tests on your BioTek instrument. The test plate is solid and therefore immune to the pipetting errors and evaporation issues, and it’s convenient size and multi-parameter functionality makes it ideal for performance test and qualification of BioTek fluorescence capable instruments. The fluorescein standard is NIST traceable.

The Fluorescence Test Plate checks for:

• Carrier ‘flatness’
• Fluorescence intensity linearity (excitation 485 nm, emission 528 nm)
• Limit of Detection for:
  • Fluorescence intensity - green (excitation 485 nm, emission 528 nm)
  • Fluorescence intensity - blue (excitation 360 nm, emission 460 nm)
  • Fluorescence polarization (excitation 485 nm, emission 528 nm)
  • Time-resolved fluorescence (excitation 360 nm, emission 620 nm)

Each Fluorescence Test Plate comes with:

• Certificate of Calibration
• Flash drive with user manual and test protocols
• Dust blower and cleaning swabs

 
A test plate recertification program is available.

BioTek recognizes the importance of verifying the performance of analytical instrumentation. Many regulatory agencies and laws such as CLIA 88 and Good Laboratory Practices (GLP) require microplate readers to be checked periodically to ensure they continue to meet factory specifications. BioTek's Test Plates make such testing easy, fast and accurate. By providing a convenient method of monitoring the performance of your reader over time, the Absorbance Test Plate can be an invaluable aid in diagnosing system performance of BioTek’s Absorbance Microplate Readers, as well as a documentation tool in your research or clinical laboratory.

All BioTek automated absorbance readers can be quickly and simply tested for critical performance specifications using two complementary methods uniquely developed by BioTek Instruments:

• BioTek's Absorbance Test Plate uses neutral density filters and precision machined alignment holes to confirm performance specs in the visible range (400 – 800 nm). It also includes a glass filter to test the wavelength accuracy of monochromator-based absorbance systems.
• BioTek's on-board Reader Diagnostic Self-Test verifies the reader's optical and electronic performance.

BioTek's Absorbance Test Plate helps meet regulatory instrumentation validation requirements by testing four critical specifications:

• Alignment
• Accuracy
• Reproducibility
• Linearity

Each Test Plate consists of the following:

• NIST (NBS)-traceable neutral density glass filters for checking accuracy, reproducibility, and linearity.
• Glass filter for monochromator accuracy.
• Blanking verification holes.
• Alignment verification holes
• Calibration data at 8 wavelengths is supplied for full range testing.

The Absorbance Test Plate includes a data sheet for these commonly used wavelengths: 405, 450, 490, 550, 620, 630, 690, and 750 nm. Custom wavelength data is available for an additional charge (Contact Customer Care for more information).

BioTek's Absorbance Test Plate complies with the IVD (In Vitro Diagnostics) Directive.

NIST-traceable luminescence test plate, for use with all Cytation and Synergy (except Synergy HT) readers. Requires updated User's Manual or corresponding Product Qualification Package (sold separately) to operate. The Luminescence Test Plate helps automate key testing as part of your instrument validation program.

Features:
NIST-traceability certificate included, guarantees a controlled light output from the test plate
Simple design, easy to use: just turn the plate on, and read the ultra-stable, low light level LEDs
Includes spare battery
Instructions for use are included in the instrument Operator’s Manuals (available for download on BioTek's Customer Resource Center). For a complete set of instructions, pre-programmed test protocols and logbooks, complete qualification procedures are also available from BioTek.

Please visit Customer Resource Center to obtain operator's manual you need.