product design

Congratulations to our 2021 winners

1st

2nd

3rd

Franziska-Schlenker
Franziska Schlenker 
Hahn Schickard-Gesellschaft für angewandte Forschung e.V.

Description: Section of fluorescence image overlay of a four-color digital droplet PCR assay for the quantification of point mutations. FAM (GFP channel, green) labeled probe for wild type sequence detection and HEX (RFP channel, blue), Atto Rho 101 (Texas Red channel, magenta) as well as Cy5 (Cy5 channel, red) labeled probes for mutation sequence detection. The overall image was composed from 72 individual images that were captured with a magnification of 2.5x in a fixed z-position.
Image captured with:
Lionheart LX

Human pluripotent stem cell-derived sympathetic neurons. Image was taken at 10x, and stained for peripherin (green), tyrosine hydroxylase (red) and DAPI (blue).
Hsueh Fu Wu
University of Georgia

Description: Human pluripotent stem cell-derived sympathetic neurons. Image was taken at 10x, and stained for peripherin (green), tyrosine hydroxylase (red) and DAPI (blue).
Image captured with:
Lionheart FX 

Human gastric epithelium stained with an Alexa fluor 488-conjugated anti-cytokeratin antibody (clone AE1/AE3) from eBioscience and nuclei co-staining using DAPI. The image was taken using a Cytation 5 (manual mode) at a 20x magnification. Karina Cereceda
Fundación Arturo López Pérez

Description: Human gastric epithelium stained with an AlexaFluor 488-conjugated anti-cytokeratin antibody (clone AE1/AE3) from eBioscience and nuclei co-staining using DAPI. The image was taken at a 20x magnification.
Image captured with:
Cytation 5


Honorable Mentions

Overlay image of bright-field and fluorescent staining for DAPI (blue stain) taken as a different way to look at cells.
Alia Mallah, University of Texas at San Antonio

Description: Bovine chondrocytes at day 21 of culture. Overlay image of bright-field and fluorescent staining for DAPI (blue stain) taken as a different way to look at cells. Image taken at 40X magnification.
Image captured with: Cytation 5

 

Human lung cancer cells attached on a coverslip. Image was taken at a 60x magnification. The cells were starved for 3 hours and then treated with Bafilomycin A overnight to inhibit autophagy. Then coverslips were fixed in 3.7% formaldehyde, stained with antibodies, counterstained with DAPI and mounted on slides using fluoromount. Bafilomycin A blocks the fusion of autophagosomes (GFP channel, anti-LC3) with the lysosomes (Texas Red channel, anti-LAMP2) which accumulate inside the cells.
Cristina Andreani, University of Cincinnati

Description: Human lung cancer cells attached on a coverslip. Image was taken at a 60x magnification. The cells were starved for 3 hours and then treated with Bafilomycin A overnight to inhibit autophagy. Then coverslips were fixed in 3.7% formaldehyde, stained with antibodies, counterstained with DAPI and mounted on slides using fluoromount. Bafilomycin A blocks the fusion of autophagosomes (GFP channel, anti-LC3) with the lysosomes (Texas Red channel, anti-LAMP2) which accumulate inside the cells. 
Image captured with: Lionheart FX

 

 Neuroblastoma cancer cells (in green) being targeted and killed by primary human natural killer cell (in red) following treatment with a pro-immunomodulatory agent.  Live cell imaging was performed using a Cytation 5 at 10x using two channel fluorescence and brightfield imaging. 
Catherine Mills, Medical University of South Carolina

Description: Neuroblastoma cancer cells (in green) being targeted and killed by primary human natural killer cell (in red) following treatment with a pro-immunomodulatory agent.  Live cell imaging was performed using a Cytation 5 at 10x using two channel fluorescence and brightfield imaging. 
Image captured with: Cytation 5

 

Cortical neuron differentiated from human pluripotent stem cell stained with Tuj1 (yellow), O-GlcNAcylation (red), mitochondria (green), and DAPI. Image was taken at a 10x magnification with automatic image processing.
Chia-Wei Huang, University of Georgia

Description: Cortical neuron differentiated from human pluripotent stem cell stained with Tuj1 (yellow), O-GlcNAcylation (red), mitochondria (green), and DAPI. Image was taken at a 10x magnification with automatic image processing.
Image captured with:
Lionheart FX

 

A 96-well fibrin/mesothelial cell assay with nuclear stain (Hoechst) and fibrin stain (647); moreover, images were taken at 4X magnification using Cytation 5.
Christian De Vera, UT Health East Texas

Description:  A 96-well fibrin/mesothelial cell assay with nuclear stain (Hoechst) and fibrin stain (647); moreover, images were taken at 4X.
Image captured with: Cytation 5

 

Immunolocalization of KCC4 (red) and H-ATPase (green) in kidney sections of mice. We can observe the cellular conformation of renal tubules and cell nuclei are stained with DAPI (Blue). Image was taken at a 20x magnification.
Lilia Guadalupe Noriega Lopez, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran

DescriptionImmunolocalization of KCC4 (red) and H-ATPase (green) in kidney sections of mice. We can observe the cellular conformation of renal tubules and cell nuclei are stained with DAPI (Blue). Image was taken at a 20x magnification.
Image captured with:
Cytation 1

 

Organotypic Peritoneal Tissue Model, Manual Mode, 10x, DAPI, Calretinin Alexa-Fluor 488, Vimentin Alexa-Fluor 594, Cell Tracker Deep Red, High-Grade Serous Ovarian Cancer cells adhering to and disrupting a confluent mesothelial monolayer.
Sabrina Ritch, McGill University

Description: Organotypic Peritoneal Tissue Model, Manual Mode, 10x, DAPI, Calretinin Alexa-Fluor 488, Vimentin Alexa-Fluor 594, Cell Tracker Deep Red, High-Grade Serous Ovarian Cancer cells adhering to and disrupting a confluent mesothelial monolayer. 
Image captured with:
Cytation 3

 

Phase Separated Fluorescein Tagged Hypophosphorylated SRSF1, 60x, GFP/brightfield overlay
Talia Fargason, University of Alabama at Birmingham

Description: Phase Separated Fluorescein Tagged Hypophosphorylated SRSF1, 60x, GFP/brightfield overlay.
Image captured with: Cytation 5

 

Autofluorescence of Mexicanum Ambystoma embryos (4X magnification, manual mode, multiplex of GFP, CY5 and DAPI channels): Mexicanum Ambystoma, also known as axolotls, are important animal models for studying tissue regeneration. Here shown is the autofluorescence image of a stage 41 axolotl embryo taken on Cytation 7 using different filters.
Yixin Xu, University of Kentucky

Description: Autofluorescence of Mexicanum Ambystoma embryos (4X magnification, manual mode, multiplex of GFP, CY5 and DAPI channels): Mexicanum Ambystoma, also known as axolotls, are important animal models for studying tissue regeneration. Here shown is the autofluorescence image of a stage 41 axolotl embryo taken on Cytation 7 using different filters.
Image captured with: Cytation 7

 


 

 

 

 

 

 

 

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