product design

Congratulations to our 2020 winners

1st

2nd

3rd

Marcus Griffiths CML277 seminal
Marcus Griffiths
Noble Research Institute LLC

Description: Maize seminal root anatomy cross-section with a lateral root emerging, 4x magnification, BioTek Lionheart FX automated 96-well plate mode, 2-week old maize seminal root embedded in 5% agar and cut at    200 µM, amplitude 2, blade angle 20 degrees using a Vibratome 150, then stained briefly in Fluorescent brightener 28 for 10 seconds highlighting the phloem and rinsed before imaging.
Image captured with:
Lionheart FX

Fireball of astrocytes- Rat astrocytes labeled with glial fibrillary acidic protein. Image captured with 20X objective as Z stack and projected by maximum fluorescence intensity.
Karthik Krishnamurthy
Thomas Jefferson University

Description: Fireball of astrocytes- Rat astrocytes labeled with glial fibrillary acidic protein. Image captured with 20x objective as z-stack and projected by maximum fluorescence intensity.
Image captured with: Cytation 5

Neural crest cells and sensory neurons differentiated from human pluripotent stem cells stained for SOX10 (magenta), TUJ1 (green), and DAPI (blue). Image was taken using a Lionheart FX (manual mode) at a 4X magnification. Kenyi Saito-Diaz
University of Georgia

Description: Neural crest cells and sensory neurons differentiated from human pluripotent stem cells stained for SOX10 (magenta), TUJ1 (green), and DAPI (blue). Image was taken using a Lionheart FX (manual mode) at a 4x magnification.
Image captured with:
Lionheart FX


Honorable Mentions

Neural crest "ridges" derived from iPS at 4x. Green: SOX10, Red: AP2a. Blue: DAPI.
Hsueh-Fu Wu, University of Georgia

Description: Neural crest "ridges" derived from iPS at 4x. Green: SOX10, Red: AP2a. Blue: DAPI.
Image captured with: Lionheart FX

 

Neural crest cells and sensory neurons differentiated from human pluripotent stem cells stained fro SOX10 (green), TUJ1 (red), DAPI (blue). Image was taken using a Lionheart FX (manual mode) at a 4X magnification.
Kenyi Saito-Diaz, University of Georgia

Description: Neural crest cells and sensory neurons differentiated from human pluripotent stem cells stained for SOX10 (green), TUJ1 (red), DAPI (blue). Image was taken using a Lionheart FX (manual mode) at a 4x magnification.
Image captured with: Lionheart FX

 

Vascularized cardiovascular organoids. Imaged with 4x objective with 8x10 stitching, three channels: GFP, RFP, and DAPI.
Huaxiao Adam Yang, Stanford University School of Medicine

Description: Vascularized cardiovascular organoids. Imaged with 4x objective with 8x10 stitching, three channels: GFP, RFP, and DAPI. An array of micropatterned vascularized cardiovascular organoids directly derived from human pluripotent stem cells.
Image captured with: Cytation 5

 

1.25x zoom, brightfield and phase contrast, Bacillus polymyxa biofilm.
Sarah Herschede, Tulane University

Description: 1.25x zoom, brightfield and phase contrast, Bacillus polymyxa biofilm.
Image captured with: Cytation 5

 

Listeria infection Raw 264.7(fixed slide sample), and different phases of cell cycle can be found in this picture
Miao Xiaofeng, School of life science and technology, Jinan University

Description:  Listeria infection Raw 264.7 (fixed slide sample), and different phases of cell cycle can be found in this picture.
Image captured with:
Lionheart FX

 

Human iPSC-derived neural progenitor cells undergoing differentiation into neurons
Angel Santiago-Lopez, Georgia Tech & Emory University

DescriptionHuman iPSC-derived neural progenitor cells undergoing differentiation into neurons. Immunocytochemistry for Beta-Tubulin III (green); Nestin (red); DAPI (blue); magnification 4x.
Image captured with:
Cytation 5

 

Angiogenic sprouts in Mimetas OrganoPlate, Cytation 5, 4X, fluorescent imaging (DAPI/RFP) with z-stacking every 25 um
Kristin Bircsak, Mimetas

Description: Angiogenic sprouts in Mimetas OrganoPlate, Cytation 5, 4x, fluorescent imaging (DAPI/GFP) with z-stacking every 25 µm, Human umbilical vein endothelial cells (HUVECs) formed a 3D tubule structure in the perfusion channel of the 3lane OrganoPlate. Cultures were fixed, stained with DAPI (blue, nuclei) and phallodin (magenta, actin), imaged along the z-axis of the culture, and focus max projections were made using the Gen5 software.
Image captured with:
Cytation 5

 

Fatty acid infiltrates in skeletal muscle
Erik Alejandro Torre Anaya, Instituto Nacional de Ciencias Medicas y Nutrician Salvador Zubiran

Description: Fatty acid infiltrates in skeletal muscle, 10x magnification, BODIPY staining (green) for fatty acids and DAPI (blue) for nuclei, we can see muscle fibers from mouse psoas, stained with BODIPY stain Fluorescent under a green UV filter at 493/503, which perfuses the fatty acids infiltrated in the muscle, while under a blue and UV filter at 358/461 we can observe the DAPI staining for the nuclei of the muscle fibers.
Image captured with: Cytation 5

 

Human iPSC-derived neuronal clusters stained with MAP2 (Alexa Fluor 555, magenta) and neurofilament (Alexa Fluor 647, red) (10X objective).
Nadia Suarez-Bosch, Illawarra Health and Medical Research Institute, University of Wollongong

Description: Human iPSC-derived neuronal clusters stained with MAP2 (Alexa Fluor 555, magenta) and neurofilament (Alexa Fluor 647, red) (10x objective).
Image captured with:
Lionheart FX

 

Rat spinal cord astrocytes transduced with mitochondria RFP (in red) and lysosome GFP (red). Images were acquired using 60X objective. Image shows Z stack projection of maximum fluorescence intensity.
Karthik Krishnamurthy, Thomas Jefferson University

DescriptionRat spinal cord astrocytes transduced with mitochondria RFP (in red) and lysosome GFP (green). Images were acquired using 60x objective. Image shows z-stack projection of maximum fluorescence intensity.
Image captured with:
Cytation 5

 

Cultured motor neurons from rat spinal cord grown for 2 weeks invitro labeled with the axonal marker neurofilament by immunocytochemistry.
Karthik Krishnamurthy, Thomas Jefferson University

Description: Cultured motor neurons from rat spinal cord grown for 2 weeks in vitro labeled with the axonal marker neurofilament by immunocytochemistry. Fluorescently labeled axons are imaged using a 20xobjective are z-stack images are maximum intensity projected.
Image captured with:
Cytation 5

 

Cytokeratin 7 (Alexa Fluor®594), and Somatostatin (Alexa Fluor® 488), with DAPI stained nuclei, of porcine islet, imaging at 20x.
Kora Grooms, Athens Encapsulation Inc.

Description: Cytokeratin 7 (Alexa Fluor®594), and Somatostatin (Alexa Fluor® 488), with DAPI stained nuclei, of porcine islet, imaging at 20x.
Image captured with:
Lionheart FX

 

Simpson Golabi Behmel Syndrome (SGBS) adipocyte cells stained with BODIPY and Hoechst 33342.
Robert Tempest, Sheffield Hallam University

Description:  Simpson Golabi Behmel Syndrome (SGBS) adipocyte cells stained with BODIPY and Hoechst 33342. Captured using 20x lens, colour brightfield. Cells were differentiated from pre-adipocytes over 14 days and stained to confirm lipid accumulation.
Image captured with: Cytation 5

 

12 month old rat spinal cord labeled for astrocyte inflammatory marker glial fibrillary acidic protein (in red).
Shashirekha Markandaiah, Thomas Jefferson University

Description:12 month old rat spinal cord labeled for astrocyte inflammatory marker glial fibrillary acidic protein (in red). Cell nucleus labeled with DAPI (in blue). Fluoresence image acquired using 20x objective.
Image captured with:
Cytation 5

 


 

 

 


 

 

 

 

 

 

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