Host Receptor Expression

Host protein receptor expression is the ultimate factor that determines if a host cell is permissible to viral entry and infection. In viral research, it is essential to determine which cell types, either primary or immortalized, are permissible to viral entry. While this can be achieved by performing infection challenges in multiple cell lines, it is more efficient to first determine if cells express the virus-specific receptor. Cells in culture can be stained for receptor expression using immunolabeling techniques. Using either the Agilent BioTek Lionheart FX automated microscope or The Agilent BioTek Cytation cell imaging multimode reader, samples can be imaged and receptor expression is determined by fluorescent intensity. Processing is done using cellular analysis tools in the Gen5 microplate reader and imager software.

Receptor Expression Application

Capture

Step 1. Capture: Samples are imaged using automated fluorescent microscopy and processed to remove background according to the Gen5 imaging protocol.

 

 

Analyze

Step 2. Analyze: Total individual cells and the cells expressing the labeled receptor are identified based on Gen5 cellular analysis parameters.

 

Report

Step 3. Report: The total cell count, positive cell count, and percent positive cells are automatically calculated and reported for each sample, along with scatterplots for data visualization.

Increase assay throughput and performance

 

Liquid handling automation with the Agilent BioTek MultiFlo FX multimode dispenser or other liquid handler can be used to automate the tedious fixing and staining steps required for immunolabeling.

BioSpa

Figure 1. The MultiFlo FX multimode dispenser.

 

Agilent BioTek BioStack compatible assay incorporates the Agilent BioTek BioStack microplate stacker enables processing and analysis of up to 50 plates.

 

BioStack

Figure 2. BioStack microplate stacker

 

 

 

 

 

 

 

Application Note

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For Research Use Only. Not for use in diagnostic procedures.
RA44218.413125

 

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