Fluorescent Microneutralization Assay

The microneutralization assay is a highly sensitive and specific assay for detecting virus-specific neutralizing antibodies and their corresponding titers that is amenable to high-density microplates. Immunofluorescence staining of a viral protein, or the use of viruses expressing a fluorescent reporter construct, provides a robust assay format for measuring the neutralizing effects of test antibodies. Using the Lionheart™ FX Automated Microscope or Cytation™ Cell Imaging Multi-Mode Reader, microplates containing target cells exposed to viral treatment and increasing concentrations of test neutralizing antibodies are imaged. Processed images are then analyzed to determine the number and fluorescence intensity of viral foci using automated Gen5™ image analysis tools.

 

Microneutralization Assay Procedure

 

Capture

Capture
Samples are imaged using automated fluorescence microscopy and then processed to reduce background signal.

 

 

Analyze

Analyze
Gen5 cellular analysis tools identify viral foci (yellow masks) using size and signal threshold criteria to detect small changes between samples.

 

Report

Report
Number and intensity of viral foci are automatically calculated and reported for each sample.

 

Increase assay throughput and performance

 

Liquid handling automation

BioSpa

The BioTek MultiFlo™ FX or other Liquid Handler can be used to automate tedious fixing and staining steps

 

BioStack™ Compatible Assay:
Process up to 50 microplates

BioStack

Incorporating the BioStack Microplate Stacker enables processing and analysis of up to 50 plates

 

BioSpa™ Compatible Assay: Live-cell analysis on up to 8 microplates

BioSpa

The BioSpa Live Cell Analysis System
enables multi-plate kinetic analysis of the
label-free TCID50 when integrated with
Cytation Live Cell Imager

 

 

 

 

 

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