Scratch Wound Assay

The wound healing or scratch assay is one of the most highly used in vitro methods to monitor and quantify collective cell migration. The Agilent BioTek AutoScratch wound making tool creates uniform and precise wounds in either 24- or 96-well plates. Cell migration into the wound area over time is monitored within the Agilent BioTek Lionheart FX automated live cell imager or Agilent BioTek Cytation cell imaging multimode reader. The Agilent BioTek Gen5 microplate reader and imager software analysis delivers detailed and reproducible cell migration results.


AutoScratch:  Automated Wound Creation for Scratch Wound Healing Assays

Automated wound creation decreases variability within and between wounds, improving repeatability of cell migration data. Combined with kinetic image capture and analysis, the Agilent BioTek Scratch Assay Starter Kit delivers a complete automated solution for conducting robust kinetic scratch wound assays.


Figure 1. The AutoScratch wound making tool can automatically create repeatable scratch wounds.

Figure 2. Clean: Walk-away procedure cleans and sterilizes pins before and after wound creation.

Scratch Assay Starter Kit
Wound: Repeatable wounds created automatically in 24- or 96-well plates with the push of a button.


Scratch Wound Assay Procedure

Cell Migration Assay: 3D Bioprinting Visual Abstract
Step 1. Capture: Kinetic high contrast brightfield (HCBF) images are captured based on desired interval and duration.


Label-Free Tumor Invasion
Step 2. Analyze: Images are processed and analyzed to measure cell migration in real-time.


Label-Free Tumor Invasion
Step 3. Report: Kinetic profiles of wound closure rates reveal detailed information on cell migration across treatment conditions.



Increase assay throughput and performance


Agilent BioTek BioSpa compatible assay performs live cell analysis on up to 8 microplates. The BioSpa live cell imaging system enables multiplate kinetic analysis of the scratch wound assay.

Liquid handling automation is achieved with the Agilent BioTek MultiFlo FX multimode dispenser.











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For Research Use Only. Not for use in diagnostic procedures.